Experiment: NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt serB and trpC are separated by 5 nucleotides trpC and trpD are separated by 97 nucleotides trpD and pabA overlap by 4 nucleotides pabA and OKGIIK_12295 are separated by 260 nucleotides
OKGIIK_12275: serB - Haloacid dehalogenase, at 2,637,954 to 2,638,628
serB
OKGIIK_12280: trpC - indole-3-glycerol phosphate synthase TrpC, at 2,638,634 to 2,639,428
trpC
OKGIIK_12285: trpD - anthranilate phosphoribosyltransferase, at 2,639,526 to 2,640,584
trpD
OKGIIK_12290: pabA - anthranilate/aminodeoxychorismate synthase component II, at 2,640,581 to 2,641,177
pabA
OKGIIK_12295: OKGIIK_12295 - TIGR02679 family protein, at 2,641,438 to 2,642,742
_12295
Position (kb)
2639
2640
2641 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2638.543 kb on + strand, within serB at 2638.543 kb on + strand, within serB at 2638.543 kb on + strand, within serB at 2638.543 kb on + strand, within serB at 2638.543 kb on + strand, within serB at 2638.543 kb on + strand, within serB at 2638.543 kb on + strand, within serB at 2638.543 kb on + strand, within serB at 2638.544 kb on - strand, within serB at 2638.544 kb on - strand, within serB at 2638.544 kb on - strand, within serB at 2638.544 kb on - strand, within serB at 2638.580 kb on - strand at 2638.734 kb on + strand, within trpC at 2638.734 kb on + strand, within trpC at 2638.734 kb on + strand, within trpC at 2638.735 kb on - strand, within trpC at 2638.735 kb on - strand, within trpC at 2638.788 kb on + strand, within trpC at 2638.789 kb on - strand, within trpC at 2638.789 kb on - strand, within trpC at 2638.789 kb on - strand, within trpC at 2638.789 kb on - strand, within trpC at 2639.181 kb on + strand, within trpC at 2639.181 kb on + strand, within trpC at 2639.181 kb on + strand, within trpC at 2639.182 kb on - strand, within trpC at 2639.182 kb on - strand, within trpC at 2639.182 kb on - strand, within trpC at 2639.182 kb on - strand, within trpC at 2639.182 kb on - strand, within trpC at 2639.182 kb on - strand, within trpC at 2639.447 kb on + strand at 2639.448 kb on - strand at 2639.448 kb on - strand at 2639.448 kb on - strand at 2639.448 kb on - strand at 2639.448 kb on - strand at 2639.448 kb on - strand at 2639.448 kb on - strand at 2639.448 kb on - strand at 2639.448 kb on - strand at 2639.448 kb on - strand at 2639.476 kb on + strand at 2639.477 kb on - strand at 2639.527 kb on + strand at 2639.527 kb on + strand at 2639.527 kb on + strand at 2639.527 kb on + strand at 2639.527 kb on + strand at 2639.527 kb on + strand at 2639.527 kb on + strand at 2639.527 kb on + strand at 2639.527 kb on + strand at 2639.527 kb on + strand at 2639.527 kb on + strand at 2639.528 kb on - strand at 2639.528 kb on - strand at 2639.528 kb on - strand at 2639.528 kb on - strand at 2639.528 kb on - strand at 2639.656 kb on + strand, within trpD at 2639.656 kb on + strand, within trpD at 2639.657 kb on - strand, within trpD at 2639.657 kb on - strand, within trpD at 2639.657 kb on - strand, within trpD at 2639.698 kb on - strand, within trpD at 2639.698 kb on - strand, within trpD at 2639.815 kb on + strand, within trpD at 2639.815 kb on + strand, within trpD at 2639.815 kb on + strand, within trpD at 2639.815 kb on + strand, within trpD at 2639.816 kb on - strand, within trpD at 2639.816 kb on - strand, within trpD at 2639.816 kb on - strand, within trpD at 2640.798 kb on + strand, within pabA at 2640.799 kb on - strand, within pabA at 2640.799 kb on - strand, within pabA at 2641.036 kb on + strand, within pabA at 2641.036 kb on + strand, within pabA at 2641.036 kb on + strand, within pabA at 2641.036 kb on + strand, within pabA at 2641.036 kb on + strand, within pabA at 2641.036 kb on + strand, within pabA at 2641.036 kb on + strand, within pabA at 2641.036 kb on + strand, within pabA at 2641.036 kb on + strand, within pabA at 2641.036 kb on + strand, within pabA at 2641.036 kb on + strand, within pabA at 2641.036 kb on + strand, within pabA at 2641.037 kb on - strand, within pabA at 2641.037 kb on - strand, within pabA at 2641.037 kb on - strand, within pabA at 2641.037 kb on - strand, within pabA at 2641.037 kb on - strand, within pabA at 2641.037 kb on - strand, within pabA at 2641.037 kb on - strand, within pabA at 2641.037 kb on - strand, within pabA at 2641.037 kb on - strand, within pabA at 2641.037 kb on - strand, within pabA at 2641.037 kb on - strand, within pabA at 2641.037 kb on - strand, within pabA at 2641.037 kb on - strand, within pabA at 2641.037 kb on - strand, within pabA at 2641.037 kb on - strand, within pabA at 2641.125 kb on + strand at 2641.125 kb on + strand at 2641.125 kb on + strand at 2641.126 kb on - strand at 2641.126 kb on - strand at 2641.140 kb on + strand at 2641.140 kb on + strand at 2641.140 kb on + strand at 2641.140 kb on + strand at 2641.140 kb on + strand at 2641.140 kb on + strand at 2641.140 kb on + strand at 2641.140 kb on + strand at 2641.140 kb on + strand at 2641.141 kb on - strand at 2641.141 kb on - strand at 2641.141 kb on - strand at 2641.141 kb on - strand at 2641.141 kb on - strand at 2641.141 kb on - strand at 2641.141 kb on - strand at 2641.141 kb on - strand at 2641.141 kb on - strand at 2641.155 kb on + strand at 2641.155 kb on + strand at 2641.155 kb on + strand at 2641.155 kb on + strand at 2641.155 kb on + strand at 2641.155 kb on + strand at 2641.155 kb on + strand at 2641.155 kb on + strand at 2641.156 kb on - strand at 2641.156 kb on - strand at 2641.156 kb on - strand at 2641.156 kb on - strand at 2641.156 kb on - strand at 2641.156 kb on - strand at 2641.156 kb on - strand at 2641.156 kb on - strand at 2641.156 kb on - strand at 2641.156 kb on - strand at 2641.156 kb on - strand at 2641.156 kb on - strand at 2641.156 kb on - strand at 2641.156 kb on - strand at 2641.156 kb on - strand at 2641.156 kb on - strand at 2641.156 kb on - strand at 2641.156 kb on - strand at 2641.156 kb on - strand at 2641.156 kb on - strand at 2641.177 kb on + strand at 2641.177 kb on + strand at 2641.177 kb on + strand at 2641.177 kb on + strand at 2641.177 kb on + strand at 2641.177 kb on + strand at 2641.177 kb on + strand at 2641.177 kb on + strand at 2641.178 kb on - strand at 2641.178 kb on - strand at 2641.178 kb on - strand at 2641.178 kb on - strand at 2641.178 kb on - strand at 2641.178 kb on - strand at 2641.178 kb on - strand at 2641.178 kb on - strand at 2641.178 kb on - strand at 2641.186 kb on + strand at 2641.186 kb on + strand at 2641.186 kb on + strand at 2641.186 kb on + strand at 2641.186 kb on + strand at 2641.186 kb on + strand at 2641.186 kb on + strand at 2641.187 kb on - strand at 2641.187 kb on - strand at 2641.187 kb on - strand at 2641.187 kb on - strand at 2641.187 kb on - strand at 2641.187 kb on - strand at 2641.187 kb on - strand at 2641.187 kb on - strand at 2641.187 kb on - strand at 2641.187 kb on - strand at 2641.204 kb on - strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.289 kb on + strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.290 kb on - strand at 2641.302 kb on - strand at 2641.302 kb on - strand at 2641.309 kb on - strand at 2641.380 kb on + strand at 2641.380 kb on + strand at 2641.380 kb on + strand at 2641.380 kb on + strand at 2641.380 kb on + strand at 2641.381 kb on - strand at 2641.381 kb on - strand at 2641.381 kb on - strand at 2641.381 kb on - strand at 2641.511 kb on + strand at 2641.511 kb on + strand at 2641.511 kb on + strand at 2641.511 kb on + strand at 2641.511 kb on + strand at 2641.512 kb on - strand at 2641.512 kb on - strand at 2641.512 kb on - strand at 2641.512 kb on - strand at 2641.548 kb on + strand at 2641.548 kb on + strand at 2641.548 kb on + strand at 2641.548 kb on + strand at 2641.548 kb on + strand at 2641.549 kb on - strand at 2641.549 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM remove 2,638,543 + serB OKGIIK_12275 0.87 -0.6 2,638,543 + serB OKGIIK_12275 0.87 -0.4 2,638,543 + serB OKGIIK_12275 0.87 +1.7 2,638,543 + serB OKGIIK_12275 0.87 +0.9 2,638,543 + serB OKGIIK_12275 0.87 -0.1 2,638,543 + serB OKGIIK_12275 0.87 +0.6 2,638,543 + serB OKGIIK_12275 0.87 +2.4 2,638,543 + serB OKGIIK_12275 0.87 -0.6 2,638,544 - serB OKGIIK_12275 0.87 -2.4 2,638,544 - serB OKGIIK_12275 0.87 -1.0 2,638,544 - serB OKGIIK_12275 0.87 +1.3 2,638,544 - serB OKGIIK_12275 0.87 +1.3 2,638,580 - -1.0 2,638,734 + trpC OKGIIK_12280 0.13 -1.3 2,638,734 + trpC OKGIIK_12280 0.13 -3.2 2,638,734 + trpC OKGIIK_12280 0.13 -2.0 2,638,735 - trpC OKGIIK_12280 0.13 -2.0 2,638,735 - trpC OKGIIK_12280 0.13 -3.4 2,638,788 + trpC OKGIIK_12280 0.19 -1.0 2,638,789 - trpC OKGIIK_12280 0.19 -2.1 2,638,789 - trpC OKGIIK_12280 0.19 -1.0 2,638,789 - trpC OKGIIK_12280 0.19 -2.0 2,638,789 - trpC OKGIIK_12280 0.19 -2.6 2,639,181 + trpC OKGIIK_12280 0.69 -2.7 2,639,181 + trpC OKGIIK_12280 0.69 -0.6 2,639,181 + trpC OKGIIK_12280 0.69 -0.6 2,639,182 - trpC OKGIIK_12280 0.69 -1.8 2,639,182 - trpC OKGIIK_12280 0.69 +1.0 2,639,182 - trpC OKGIIK_12280 0.69 +0.0 2,639,182 - trpC OKGIIK_12280 0.69 -2.0 2,639,182 - trpC OKGIIK_12280 0.69 -0.6 2,639,182 - trpC OKGIIK_12280 0.69 +0.0 2,639,447 + -1.2 2,639,448 - -0.2 2,639,448 - +2.4 2,639,448 - +0.2 2,639,448 - -0.4 2,639,448 - +0.6 2,639,448 - -1.5 2,639,448 - -0.5 2,639,448 - +0.0 2,639,448 - +0.8 2,639,448 - +0.0 2,639,476 + +1.0 2,639,477 - +1.4 2,639,527 + -0.6 2,639,527 + +0.4 2,639,527 + -0.2 2,639,527 + -0.2 2,639,527 + -1.6 2,639,527 + -0.4 2,639,527 + +0.5 2,639,527 + +0.0 2,639,527 + +0.8 2,639,527 + -1.6 2,639,527 + +0.2 2,639,528 - -0.6 2,639,528 - -0.2 2,639,528 - -1.0 2,639,528 - -0.6 2,639,528 - +1.1 2,639,656 + trpD OKGIIK_12285 0.12 -3.6 2,639,656 + trpD OKGIIK_12285 0.12 -1.3 2,639,657 - trpD OKGIIK_12285 0.12 -1.2 2,639,657 - trpD OKGIIK_12285 0.12 -1.3 2,639,657 - trpD OKGIIK_12285 0.12 -2.7 2,639,698 - trpD OKGIIK_12285 0.16 +1.0 2,639,698 - trpD OKGIIK_12285 0.16 -1.0 2,639,815 + trpD OKGIIK_12285 0.27 -2.0 2,639,815 + trpD OKGIIK_12285 0.27 -1.8 2,639,815 + trpD OKGIIK_12285 0.27 +1.0 2,639,815 + trpD OKGIIK_12285 0.27 -2.3 2,639,816 - trpD OKGIIK_12285 0.27 -2.2 2,639,816 - trpD OKGIIK_12285 0.27 -2.7 2,639,816 - trpD OKGIIK_12285 0.27 -2.9 2,640,798 + pabA OKGIIK_12290 0.36 -0.6 2,640,799 - pabA OKGIIK_12290 0.37 -2.8 2,640,799 - pabA OKGIIK_12290 0.37 -2.0 2,641,036 + pabA OKGIIK_12290 0.76 -3.0 2,641,036 + pabA OKGIIK_12290 0.76 -2.3 2,641,036 + pabA OKGIIK_12290 0.76 -0.6 2,641,036 + pabA OKGIIK_12290 0.76 -3.7 2,641,036 + pabA OKGIIK_12290 0.76 -0.6 2,641,036 + pabA OKGIIK_12290 0.76 -2.2 2,641,036 + pabA OKGIIK_12290 0.76 +1.0 2,641,036 + pabA OKGIIK_12290 0.76 -1.3 2,641,036 + pabA OKGIIK_12290 0.76 -3.0 2,641,036 + pabA OKGIIK_12290 0.76 +0.0 2,641,036 + pabA OKGIIK_12290 0.76 +0.0 2,641,036 + pabA OKGIIK_12290 0.76 +0.0 2,641,037 - pabA OKGIIK_12290 0.76 -2.0 2,641,037 - pabA OKGIIK_12290 0.76 -2.3 2,641,037 - pabA OKGIIK_12290 0.76 -1.3 2,641,037 - pabA OKGIIK_12290 0.76 -0.6 2,641,037 - pabA OKGIIK_12290 0.76 -0.3 2,641,037 - pabA OKGIIK_12290 0.76 -1.0 2,641,037 - pabA OKGIIK_12290 0.76 -2.6 2,641,037 - pabA OKGIIK_12290 0.76 -0.6 2,641,037 - pabA OKGIIK_12290 0.76 +0.0 2,641,037 - pabA OKGIIK_12290 0.76 -1.8 2,641,037 - pabA OKGIIK_12290 0.76 -2.4 2,641,037 - pabA OKGIIK_12290 0.76 -1.2 2,641,037 - pabA OKGIIK_12290 0.76 -0.3 2,641,037 - pabA OKGIIK_12290 0.76 -0.6 2,641,037 - pabA OKGIIK_12290 0.76 -2.2 2,641,125 + -2.3 2,641,125 + -1.8 2,641,125 + -1.8 2,641,126 - -0.6 2,641,126 - +1.0 2,641,140 + -2.0 2,641,140 + -0.3 2,641,140 + -2.6 2,641,140 + -3.2 2,641,140 + -1.0 2,641,140 + -1.0 2,641,140 + -2.7 2,641,140 + -1.6 2,641,140 + -1.3 2,641,141 - -1.6 2,641,141 - -1.8 2,641,141 - -2.9 2,641,141 - -2.0 2,641,141 - -2.6 2,641,141 - +2.0 2,641,141 - -1.0 2,641,141 - -2.2 2,641,141 - -2.8 2,641,155 + -2.9 2,641,155 + -0.6 2,641,155 + -3.0 2,641,155 + -2.9 2,641,155 + +1.0 2,641,155 + -1.0 2,641,155 + -3.2 2,641,155 + -1.6 2,641,156 - -1.8 2,641,156 - -2.2 2,641,156 - -1.0 2,641,156 - -1.3 2,641,156 - -4.2 2,641,156 - -0.6 2,641,156 - +1.8 2,641,156 - -1.5 2,641,156 - +0.0 2,641,156 - -1.0 2,641,156 - -1.6 2,641,156 - -2.0 2,641,156 - -2.2 2,641,156 - -2.3 2,641,156 - -3.1 2,641,156 - -1.3 2,641,156 - -1.0 2,641,156 - -1.6 2,641,156 - -2.8 2,641,156 - -3.0 2,641,177 + -0.4 2,641,177 + -0.6 2,641,177 + -1.6 2,641,177 + -2.2 2,641,177 + -0.2 2,641,177 + +0.0 2,641,177 + -0.6 2,641,177 + -2.3 2,641,178 - -0.3 2,641,178 - -2.0 2,641,178 - +0.5 2,641,178 - -0.2 2,641,178 - -0.6 2,641,178 - +0.4 2,641,178 - -0.6 2,641,178 - +1.3 2,641,178 - -0.1 2,641,186 + -1.3 2,641,186 + -0.8 2,641,186 + -1.0 2,641,186 + -1.3 2,641,186 + -2.0 2,641,186 + -2.6 2,641,186 + -0.8 2,641,187 - +0.1 2,641,187 - -0.8 2,641,187 - -1.6 2,641,187 - +0.7 2,641,187 - -1.6 2,641,187 - -1.2 2,641,187 - +0.1 2,641,187 - -0.4 2,641,187 - +0.7 2,641,187 - +0.3 2,641,204 - +0.7 2,641,289 + +0.7 2,641,289 + -2.6 2,641,289 + -1.7 2,641,289 + -1.8 2,641,289 + +0.7 2,641,289 + -0.6 2,641,289 + -2.2 2,641,289 + -0.6 2,641,289 + -1.0 2,641,289 + +1.3 2,641,289 + -1.0 2,641,289 + -0.7 2,641,289 + -0.6 2,641,289 + -2.3 2,641,289 + -2.4 2,641,289 + -1.1 2,641,289 + -0.6 2,641,289 + -0.6 2,641,289 + +1.4 2,641,289 + +0.0 2,641,289 + -1.8 2,641,289 + -0.4 2,641,289 + -0.1 2,641,289 + -0.7 2,641,289 + -1.8 2,641,290 - +0.6 2,641,290 - -1.6 2,641,290 - +0.8 2,641,290 - -0.2 2,641,290 - +1.3 2,641,290 - +0.0 2,641,290 - +2.0 2,641,290 - -1.6 2,641,290 - +2.6 2,641,290 - +1.5 2,641,290 - +0.0 2,641,290 - +0.6 2,641,290 - +0.8 2,641,290 - -0.6 2,641,290 - -1.3 2,641,290 - -1.2 2,641,290 - -1.3 2,641,290 - -0.2 2,641,290 - -0.4 2,641,290 - -0.3 2,641,290 - +1.3 2,641,290 - -1.0 2,641,290 - -2.0 2,641,290 - +0.2 2,641,290 - -0.8 2,641,290 - +1.3 2,641,290 - -0.3 2,641,290 - -0.6 2,641,290 - +1.6 2,641,290 - -1.0 2,641,302 - +0.1 2,641,302 - +0.0 2,641,309 - +0.3 2,641,380 + -1.0 2,641,380 + -1.3 2,641,380 + -3.1 2,641,380 + -0.6 2,641,380 + +0.5 2,641,381 - +1.0 2,641,381 - +2.6 2,641,381 - -0.6 2,641,381 - +0.0 2,641,511 + +1.0 2,641,511 + -0.1 2,641,511 + -1.3 2,641,511 + +0.6 2,641,511 + +0.2 2,641,512 - +2.0 2,641,512 - +2.0 2,641,512 - +2.4 2,641,512 - -1.0 2,641,548 + +1.0 2,641,548 + -1.0 2,641,548 + +0.7 2,641,548 + +0.7 2,641,548 + -1.8 2,641,549 - -0.3 2,641,549 - +0.0
Or see this region's nucleotide sequence