Experiment: NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nusB and ribE overlap by 1 nucleotides ribE and ldcA are separated by 220 nucleotides ldcA and ribB are separated by 177 nucleotides
OKGIIK_11405: nusB - transcription antitermination factor NusB, at 2,449,821 to 2,450,267
nusB
OKGIIK_11410: ribE - 6,7-dimethyl-8-ribityllumazine synthase, at 2,450,267 to 2,450,728
ribE
OKGIIK_11415: ldcA - muramoyltetrapeptide carboxypeptidase, at 2,450,949 to 2,451,893
ldcA
OKGIIK_11420: ribB - bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II, at 2,452,071 to 2,453,162
ribB
Position (kb)
2450
2451
2452 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2450.777 kb on - strand at 2450.875 kb on + strand at 2450.875 kb on + strand at 2450.875 kb on + strand at 2450.875 kb on + strand at 2450.876 kb on - strand at 2450.876 kb on - strand at 2450.887 kb on + strand at 2450.887 kb on + strand at 2450.887 kb on + strand at 2450.887 kb on + strand at 2450.888 kb on - strand at 2450.888 kb on - strand at 2450.888 kb on - strand at 2450.888 kb on - strand at 2450.888 kb on - strand at 2450.888 kb on - strand at 2450.888 kb on - strand at 2451.136 kb on + strand, within ldcA at 2451.137 kb on - strand, within ldcA at 2451.137 kb on - strand, within ldcA at 2451.137 kb on - strand, within ldcA at 2451.137 kb on - strand, within ldcA at 2451.137 kb on - strand, within ldcA at 2451.137 kb on - strand, within ldcA at 2451.137 kb on - strand, within ldcA at 2451.137 kb on - strand, within ldcA at 2451.229 kb on + strand, within ldcA at 2451.229 kb on + strand, within ldcA at 2451.229 kb on + strand, within ldcA at 2451.229 kb on + strand, within ldcA at 2451.230 kb on - strand, within ldcA at 2451.230 kb on - strand, within ldcA at 2451.230 kb on - strand, within ldcA at 2451.230 kb on - strand, within ldcA at 2451.230 kb on - strand, within ldcA at 2451.230 kb on - strand, within ldcA at 2451.322 kb on + strand, within ldcA at 2451.322 kb on + strand, within ldcA at 2451.322 kb on + strand, within ldcA at 2451.322 kb on + strand, within ldcA at 2451.322 kb on + strand, within ldcA at 2451.323 kb on - strand, within ldcA at 2451.323 kb on - strand, within ldcA at 2451.323 kb on - strand, within ldcA at 2451.323 kb on - strand, within ldcA at 2451.323 kb on - strand, within ldcA at 2451.625 kb on + strand, within ldcA at 2451.625 kb on + strand, within ldcA at 2451.626 kb on - strand, within ldcA at 2451.626 kb on - strand, within ldcA at 2451.626 kb on - strand, within ldcA at 2451.626 kb on - strand, within ldcA at 2451.626 kb on - strand, within ldcA at 2451.922 kb on + strand at 2451.922 kb on + strand at 2451.922 kb on + strand at 2451.922 kb on + strand at 2451.922 kb on + strand at 2451.923 kb on - strand at 2451.923 kb on - strand at 2451.923 kb on - strand at 2451.923 kb on - strand at 2451.923 kb on - strand at 2451.923 kb on - strand at 2451.923 kb on - strand at 2451.923 kb on - strand at 2451.923 kb on - strand at 2451.923 kb on - strand at 2451.923 kb on - strand at 2451.985 kb on - strand at 2452.030 kb on + strand at 2452.030 kb on + strand at 2452.030 kb on + strand at 2452.031 kb on - strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.079 kb on - strand at 2452.391 kb on + strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.457 kb on - strand, within ribB at 2452.457 kb on - strand, within ribB at 2452.457 kb on - strand, within ribB at 2452.457 kb on - strand, within ribB
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM remove 2,450,777 - -0.8 2,450,875 + -0.0 2,450,875 + -1.3 2,450,875 + -1.3 2,450,875 + -0.7 2,450,876 - -1.8 2,450,876 - -2.9 2,450,887 + -2.0 2,450,887 + +0.6 2,450,887 + +0.5 2,450,887 + -0.8 2,450,888 - -0.4 2,450,888 - -1.0 2,450,888 - -0.5 2,450,888 - -0.3 2,450,888 - +0.7 2,450,888 - +0.4 2,450,888 - -0.7 2,451,136 + ldcA OKGIIK_11415 0.20 -1.8 2,451,137 - ldcA OKGIIK_11415 0.20 +1.0 2,451,137 - ldcA OKGIIK_11415 0.20 -2.6 2,451,137 - ldcA OKGIIK_11415 0.20 -1.8 2,451,137 - ldcA OKGIIK_11415 0.20 -0.6 2,451,137 - ldcA OKGIIK_11415 0.20 -1.0 2,451,137 - ldcA OKGIIK_11415 0.20 -2.2 2,451,137 - ldcA OKGIIK_11415 0.20 -0.0 2,451,137 - ldcA OKGIIK_11415 0.20 -0.6 2,451,229 + ldcA OKGIIK_11415 0.30 -0.6 2,451,229 + ldcA OKGIIK_11415 0.30 +1.4 2,451,229 + ldcA OKGIIK_11415 0.30 -1.3 2,451,229 + ldcA OKGIIK_11415 0.30 -2.9 2,451,230 - ldcA OKGIIK_11415 0.30 -1.3 2,451,230 - ldcA OKGIIK_11415 0.30 -0.0 2,451,230 - ldcA OKGIIK_11415 0.30 -1.8 2,451,230 - ldcA OKGIIK_11415 0.30 -1.0 2,451,230 - ldcA OKGIIK_11415 0.30 -0.0 2,451,230 - ldcA OKGIIK_11415 0.30 -0.6 2,451,322 + ldcA OKGIIK_11415 0.39 -1.3 2,451,322 + ldcA OKGIIK_11415 0.39 -1.2 2,451,322 + ldcA OKGIIK_11415 0.39 -1.3 2,451,322 + ldcA OKGIIK_11415 0.39 -0.0 2,451,322 + ldcA OKGIIK_11415 0.39 -1.0 2,451,323 - ldcA OKGIIK_11415 0.40 -1.0 2,451,323 - ldcA OKGIIK_11415 0.40 -1.3 2,451,323 - ldcA OKGIIK_11415 0.40 -1.8 2,451,323 - ldcA OKGIIK_11415 0.40 +1.0 2,451,323 - ldcA OKGIIK_11415 0.40 -0.0 2,451,625 + ldcA OKGIIK_11415 0.72 -2.8 2,451,625 + ldcA OKGIIK_11415 0.72 +0.4 2,451,626 - ldcA OKGIIK_11415 0.72 -1.3 2,451,626 - ldcA OKGIIK_11415 0.72 -2.7 2,451,626 - ldcA OKGIIK_11415 0.72 -1.6 2,451,626 - ldcA OKGIIK_11415 0.72 -1.6 2,451,626 - ldcA OKGIIK_11415 0.72 -0.6 2,451,922 + +0.7 2,451,922 + +1.0 2,451,922 + -0.0 2,451,922 + +1.4 2,451,922 + -2.0 2,451,923 - +0.3 2,451,923 - +0.6 2,451,923 - -1.6 2,451,923 - -0.6 2,451,923 - +2.6 2,451,923 - +0.3 2,451,923 - -0.7 2,451,923 - -1.4 2,451,923 - -0.3 2,451,923 - +0.6 2,451,923 - -0.8 2,451,985 - -1.1 2,452,030 + -0.3 2,452,030 + +0.7 2,452,030 + +1.6 2,452,031 - -1.6 2,452,078 + +0.4 2,452,078 + -0.0 2,452,078 + -1.3 2,452,078 + -0.0 2,452,078 + -0.4 2,452,078 + +1.0 2,452,078 + +2.0 2,452,078 + +0.4 2,452,078 + -0.8 2,452,078 + +1.4 2,452,078 + +2.8 2,452,078 + -0.2 2,452,078 + -0.0 2,452,078 + -0.0 2,452,078 + -0.0 2,452,078 + -1.6 2,452,079 - -0.2 2,452,391 + ribB OKGIIK_11420 0.29 +0.4 2,452,392 - ribB OKGIIK_11420 0.29 -0.0 2,452,392 - ribB OKGIIK_11420 0.29 +0.1 2,452,392 - ribB OKGIIK_11420 0.29 -0.6 2,452,392 - ribB OKGIIK_11420 0.29 -0.6 2,452,392 - ribB OKGIIK_11420 0.29 -1.1 2,452,392 - ribB OKGIIK_11420 0.29 +0.2 2,452,392 - ribB OKGIIK_11420 0.29 -0.4 2,452,392 - ribB OKGIIK_11420 0.29 -0.5 2,452,392 - ribB OKGIIK_11420 0.29 -1.3 2,452,392 - ribB OKGIIK_11420 0.29 +1.0 2,452,392 - ribB OKGIIK_11420 0.29 -0.6 2,452,392 - ribB OKGIIK_11420 0.29 +0.4 2,452,392 - ribB OKGIIK_11420 0.29 -0.1 2,452,392 - ribB OKGIIK_11420 0.29 -0.6 2,452,392 - ribB OKGIIK_11420 0.29 -2.3 2,452,392 - ribB OKGIIK_11420 0.29 -1.2 2,452,392 - ribB OKGIIK_11420 0.29 +1.0 2,452,392 - ribB OKGIIK_11420 0.29 -1.0 2,452,392 - ribB OKGIIK_11420 0.29 -1.2 2,452,457 - ribB OKGIIK_11420 0.35 -0.6 2,452,457 - ribB OKGIIK_11420 0.35 -0.0 2,452,457 - ribB OKGIIK_11420 0.35 +2.1 2,452,457 - ribB OKGIIK_11420 0.35 -0.0
Or see this region's nucleotide sequence