Experiment: NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lysR and greA are separated by 292 nucleotides greA and carB overlap by 4 nucleotides carB and OKGIIK_07300 are separated by 1 nucleotides
OKGIIK_07285: lysR - LysR family transcriptional regulator, at 1,610,856 to 1,611,767
lysR
OKGIIK_07290: greA - transcription elongation factor GreA, at 1,612,060 to 1,612,536
greA
OKGIIK_07295: carB - carbamoyl-phosphate synthase large subunit, at 1,612,533 to 1,615,760
carB
OKGIIK_07300: OKGIIK_07300 - carbamoyl phosphate synthase small subunit, at 1,615,762 to 1,616,889
_07300
Position (kb)
1612
1613
1614
1615
1616 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1611.639 kb on + strand, within lysR at 1611.639 kb on + strand, within lysR at 1611.640 kb on - strand, within lysR at 1611.640 kb on - strand, within lysR at 1611.785 kb on + strand at 1612.067 kb on + strand at 1612.067 kb on + strand at 1612.067 kb on + strand at 1612.067 kb on + strand at 1612.067 kb on + strand at 1612.067 kb on + strand at 1612.067 kb on + strand at 1612.067 kb on + strand at 1612.067 kb on + strand at 1612.067 kb on + strand at 1612.067 kb on + strand at 1612.068 kb on - strand at 1612.068 kb on - strand at 1612.068 kb on - strand at 1612.068 kb on - strand at 1612.068 kb on - strand at 1612.068 kb on - strand at 1612.088 kb on + strand at 1612.088 kb on + strand at 1612.088 kb on + strand at 1612.088 kb on + strand at 1612.088 kb on + strand at 1612.088 kb on + strand at 1612.089 kb on - strand at 1612.089 kb on - strand at 1612.089 kb on - strand at 1612.089 kb on - strand at 1612.089 kb on - strand at 1612.223 kb on + strand, within greA at 1612.223 kb on + strand, within greA at 1612.223 kb on + strand, within greA at 1612.223 kb on + strand, within greA at 1612.223 kb on + strand, within greA at 1612.223 kb on + strand, within greA at 1612.224 kb on - strand, within greA at 1612.224 kb on - strand, within greA at 1612.224 kb on - strand, within greA at 1612.224 kb on - strand, within greA at 1612.224 kb on - strand, within greA at 1612.224 kb on - strand, within greA at 1612.224 kb on - strand, within greA at 1612.224 kb on - strand, within greA at 1612.224 kb on - strand, within greA at 1612.224 kb on - strand, within greA at 1612.224 kb on - strand, within greA at 1612.224 kb on - strand, within greA at 1612.224 kb on - strand, within greA at 1612.224 kb on - strand, within greA at 1612.394 kb on + strand, within greA at 1612.394 kb on + strand, within greA at 1612.394 kb on + strand, within greA at 1612.394 kb on + strand, within greA at 1612.394 kb on + strand, within greA at 1612.394 kb on + strand, within greA at 1612.395 kb on - strand, within greA at 1612.395 kb on - strand, within greA at 1612.639 kb on + strand at 1612.640 kb on - strand at 1612.722 kb on - strand at 1612.723 kb on + strand at 1612.724 kb on - strand at 1612.819 kb on + strand at 1613.098 kb on + strand, within carB at 1613.098 kb on + strand, within carB at 1613.362 kb on + strand, within carB at 1613.463 kb on + strand, within carB at 1613.464 kb on - strand, within carB at 1613.464 kb on - strand, within carB at 1613.552 kb on - strand, within carB at 1613.575 kb on + strand, within carB at 1613.608 kb on + strand, within carB at 1613.608 kb on + strand, within carB at 1613.609 kb on - strand, within carB at 1613.609 kb on - strand, within carB at 1613.609 kb on - strand, within carB at 1613.836 kb on + strand, within carB at 1613.911 kb on + strand, within carB at 1613.912 kb on - strand, within carB at 1613.933 kb on - strand, within carB at 1614.118 kb on + strand, within carB at 1614.118 kb on + strand, within carB at 1614.127 kb on + strand, within carB at 1614.128 kb on - strand, within carB at 1614.133 kb on + strand, within carB at 1614.134 kb on - strand, within carB at 1614.181 kb on + strand, within carB at 1614.181 kb on + strand, within carB at 1614.400 kb on + strand, within carB at 1614.400 kb on + strand, within carB at 1614.401 kb on - strand, within carB at 1614.401 kb on - strand, within carB at 1614.401 kb on - strand, within carB at 1614.401 kb on - strand, within carB at 1614.401 kb on - strand, within carB at 1614.701 kb on - strand, within carB at 1614.701 kb on - strand, within carB at 1614.701 kb on - strand, within carB at 1614.701 kb on - strand, within carB at 1614.979 kb on + strand, within carB at 1615.213 kb on + strand, within carB at 1615.429 kb on + strand, within carB at 1615.567 kb on + strand at 1615.567 kb on + strand at 1615.568 kb on - strand at 1615.764 kb on - strand at 1615.764 kb on - strand at 1615.764 kb on - strand at 1615.764 kb on - strand at 1615.805 kb on + strand at 1615.806 kb on - strand at 1615.806 kb on - strand at 1615.806 kb on - strand at 1615.806 kb on - strand at 1615.806 kb on - strand at 1615.806 kb on - strand at 1615.806 kb on - strand at 1615.911 kb on + strand, within OKGIIK_07300 at 1615.911 kb on + strand, within OKGIIK_07300 at 1615.912 kb on - strand, within OKGIIK_07300 at 1616.151 kb on - strand, within OKGIIK_07300 at 1616.309 kb on + strand, within OKGIIK_07300 at 1616.366 kb on + strand, within OKGIIK_07300 at 1616.367 kb on - strand, within OKGIIK_07300 at 1616.720 kb on + strand, within OKGIIK_07300 at 1616.720 kb on + strand, within OKGIIK_07300 at 1616.747 kb on + strand, within OKGIIK_07300 at 1616.747 kb on + strand, within OKGIIK_07300
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM remove 1,611,639 + lysR OKGIIK_07285 0.86 +0.0 1,611,639 + lysR OKGIIK_07285 0.86 -0.9 1,611,640 - lysR OKGIIK_07285 0.86 -1.4 1,611,640 - lysR OKGIIK_07285 0.86 +0.3 1,611,785 + +1.9 1,612,067 + +1.1 1,612,067 + -2.3 1,612,067 + -1.4 1,612,067 + -1.3 1,612,067 + +0.6 1,612,067 + +0.7 1,612,067 + -1.1 1,612,067 + -1.1 1,612,067 + +3.1 1,612,067 + +0.9 1,612,067 + -0.7 1,612,068 - -0.3 1,612,068 - +0.3 1,612,068 - +0.7 1,612,068 - -1.6 1,612,068 - -0.9 1,612,068 - +0.1 1,612,088 + -0.4 1,612,088 + +0.5 1,612,088 + +0.6 1,612,088 + -0.1 1,612,088 + +0.5 1,612,088 + -2.1 1,612,089 - +0.9 1,612,089 - +0.9 1,612,089 - +1.1 1,612,089 - -1.4 1,612,089 - +1.2 1,612,223 + greA OKGIIK_07290 0.34 -0.4 1,612,223 + greA OKGIIK_07290 0.34 +0.6 1,612,223 + greA OKGIIK_07290 0.34 +0.1 1,612,223 + greA OKGIIK_07290 0.34 +0.9 1,612,223 + greA OKGIIK_07290 0.34 -0.9 1,612,223 + greA OKGIIK_07290 0.34 +0.7 1,612,224 - greA OKGIIK_07290 0.34 +1.2 1,612,224 - greA OKGIIK_07290 0.34 +0.8 1,612,224 - greA OKGIIK_07290 0.34 -0.4 1,612,224 - greA OKGIIK_07290 0.34 +1.3 1,612,224 - greA OKGIIK_07290 0.34 -0.7 1,612,224 - greA OKGIIK_07290 0.34 +0.9 1,612,224 - greA OKGIIK_07290 0.34 -1.0 1,612,224 - greA OKGIIK_07290 0.34 +2.1 1,612,224 - greA OKGIIK_07290 0.34 +1.0 1,612,224 - greA OKGIIK_07290 0.34 -0.7 1,612,224 - greA OKGIIK_07290 0.34 +2.5 1,612,224 - greA OKGIIK_07290 0.34 -0.1 1,612,224 - greA OKGIIK_07290 0.34 +0.6 1,612,224 - greA OKGIIK_07290 0.34 -0.9 1,612,394 + greA OKGIIK_07290 0.70 +2.6 1,612,394 + greA OKGIIK_07290 0.70 +0.0 1,612,394 + greA OKGIIK_07290 0.70 +0.2 1,612,394 + greA OKGIIK_07290 0.70 +0.7 1,612,394 + greA OKGIIK_07290 0.70 +1.6 1,612,394 + greA OKGIIK_07290 0.70 +0.9 1,612,395 - greA OKGIIK_07290 0.70 +2.7 1,612,395 - greA OKGIIK_07290 0.70 -0.3 1,612,639 + -1.7 1,612,640 - -0.1 1,612,722 - -0.7 1,612,723 + -2.1 1,612,724 - -1.7 1,612,819 + -0.1 1,613,098 + carB OKGIIK_07295 0.18 -0.7 1,613,098 + carB OKGIIK_07295 0.18 -1.7 1,613,362 + carB OKGIIK_07295 0.26 -1.7 1,613,463 + carB OKGIIK_07295 0.29 -0.1 1,613,464 - carB OKGIIK_07295 0.29 -1.7 1,613,464 - carB OKGIIK_07295 0.29 -0.1 1,613,552 - carB OKGIIK_07295 0.32 -1.1 1,613,575 + carB OKGIIK_07295 0.32 -3.3 1,613,608 + carB OKGIIK_07295 0.33 -0.7 1,613,608 + carB OKGIIK_07295 0.33 -2.3 1,613,609 - carB OKGIIK_07295 0.33 +0.9 1,613,609 - carB OKGIIK_07295 0.33 -1.4 1,613,609 - carB OKGIIK_07295 0.33 -1.1 1,613,836 + carB OKGIIK_07295 0.40 -2.1 1,613,911 + carB OKGIIK_07295 0.43 +0.9 1,613,912 - carB OKGIIK_07295 0.43 -1.4 1,613,933 - carB OKGIIK_07295 0.43 -1.9 1,614,118 + carB OKGIIK_07295 0.49 +0.4 1,614,118 + carB OKGIIK_07295 0.49 -1.1 1,614,127 + carB OKGIIK_07295 0.49 -0.1 1,614,128 - carB OKGIIK_07295 0.49 -2.3 1,614,133 + carB OKGIIK_07295 0.50 -1.9 1,614,134 - carB OKGIIK_07295 0.50 -2.1 1,614,181 + carB OKGIIK_07295 0.51 -1.7 1,614,181 + carB OKGIIK_07295 0.51 -0.7 1,614,400 + carB OKGIIK_07295 0.58 -0.7 1,614,400 + carB OKGIIK_07295 0.58 -1.1 1,614,401 - carB OKGIIK_07295 0.58 -1.1 1,614,401 - carB OKGIIK_07295 0.58 -3.6 1,614,401 - carB OKGIIK_07295 0.58 +0.1 1,614,401 - carB OKGIIK_07295 0.58 -0.1 1,614,401 - carB OKGIIK_07295 0.58 -0.7 1,614,701 - carB OKGIIK_07295 0.67 +0.9 1,614,701 - carB OKGIIK_07295 0.67 -1.4 1,614,701 - carB OKGIIK_07295 0.67 -1.9 1,614,701 - carB OKGIIK_07295 0.67 -1.1 1,614,979 + carB OKGIIK_07295 0.76 +0.9 1,615,213 + carB OKGIIK_07295 0.83 -1.4 1,615,429 + carB OKGIIK_07295 0.90 -1.4 1,615,567 + -1.7 1,615,567 + -1.4 1,615,568 - -1.9 1,615,764 - -1.4 1,615,764 - -0.7 1,615,764 - -1.9 1,615,764 - -0.4 1,615,805 + +0.9 1,615,806 - -0.1 1,615,806 - -4.4 1,615,806 - -0.3 1,615,806 - -0.1 1,615,806 - -1.7 1,615,806 - -0.8 1,615,806 - -1.1 1,615,911 + OKGIIK_07300 0.13 -2.3 1,615,911 + OKGIIK_07300 0.13 -0.7 1,615,912 - OKGIIK_07300 0.13 +2.2 1,616,151 - OKGIIK_07300 0.34 -1.7 1,616,309 + OKGIIK_07300 0.48 -1.7 1,616,366 + OKGIIK_07300 0.54 -1.7 1,616,367 - OKGIIK_07300 0.54 -0.7 1,616,720 + OKGIIK_07300 0.85 -0.4 1,616,720 + OKGIIK_07300 0.85 -1.4 1,616,747 + OKGIIK_07300 0.87 -1.4 1,616,747 + OKGIIK_07300 0.87 -1.4
Or see this region's nucleotide sequence