Experiment: NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_04935 and yerC are separated by 289 nucleotides yerC and hisG overlap by 4 nucleotides hisG and hisD are separated by 28 nucleotides
OKGIIK_04935: OKGIIK_04935 - hypothetical protein, at 1,071,696 to 1,072,709
_04935
OKGIIK_04940: yerC - TrpR , YerC/YecD, at 1,072,999 to 1,073,334
yerC
OKGIIK_04945: hisG - ATP phosphoribosyltransferase, at 1,073,331 to 1,074,224
hisG
OKGIIK_04950: hisD - histidinol dehydrogenase, at 1,074,253 to 1,075,551
hisD
Position (kb)
1073
1074
1075 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1072.354 kb on + strand, within OKGIIK_04935 at 1072.354 kb on + strand, within OKGIIK_04935 at 1072.354 kb on + strand, within OKGIIK_04935 at 1072.355 kb on - strand, within OKGIIK_04935 at 1072.369 kb on + strand, within OKGIIK_04935 at 1072.369 kb on + strand, within OKGIIK_04935 at 1072.369 kb on + strand, within OKGIIK_04935 at 1072.369 kb on + strand, within OKGIIK_04935 at 1072.370 kb on - strand, within OKGIIK_04935 at 1072.370 kb on - strand, within OKGIIK_04935 at 1072.370 kb on - strand, within OKGIIK_04935 at 1072.370 kb on - strand, within OKGIIK_04935 at 1072.433 kb on - strand, within OKGIIK_04935 at 1072.457 kb on + strand, within OKGIIK_04935 at 1072.492 kb on + strand, within OKGIIK_04935 at 1072.492 kb on + strand, within OKGIIK_04935 at 1072.492 kb on + strand, within OKGIIK_04935 at 1072.492 kb on + strand, within OKGIIK_04935 at 1072.492 kb on + strand, within OKGIIK_04935 at 1072.492 kb on + strand, within OKGIIK_04935 at 1072.492 kb on + strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.534 kb on + strand, within OKGIIK_04935 at 1072.534 kb on + strand, within OKGIIK_04935 at 1072.534 kb on + strand, within OKGIIK_04935 at 1072.534 kb on + strand, within OKGIIK_04935 at 1072.535 kb on - strand, within OKGIIK_04935 at 1072.535 kb on - strand, within OKGIIK_04935 at 1072.535 kb on - strand, within OKGIIK_04935 at 1072.535 kb on - strand, within OKGIIK_04935 at 1072.690 kb on + strand at 1072.690 kb on + strand at 1072.690 kb on + strand at 1072.691 kb on - strand at 1072.691 kb on - strand at 1072.691 kb on - strand at 1072.691 kb on - strand at 1072.691 kb on - strand at 1072.691 kb on - strand at 1072.691 kb on - strand at 1072.691 kb on - strand at 1072.691 kb on - strand at 1072.691 kb on - strand at 1072.691 kb on - strand at 1072.691 kb on - strand at 1072.835 kb on + strand at 1072.835 kb on + strand at 1072.836 kb on - strand at 1072.912 kb on + strand at 1072.912 kb on + strand at 1072.912 kb on + strand at 1072.912 kb on + strand at 1072.912 kb on + strand at 1072.912 kb on + strand at 1072.913 kb on - strand at 1072.913 kb on - strand at 1072.913 kb on - strand at 1072.913 kb on - strand at 1072.913 kb on - strand at 1072.913 kb on - strand at 1072.962 kb on + strand at 1072.962 kb on + strand at 1072.962 kb on + strand at 1072.962 kb on + strand at 1072.962 kb on + strand at 1072.962 kb on + strand at 1072.962 kb on + strand at 1072.963 kb on - strand at 1072.963 kb on - strand at 1072.963 kb on - strand at 1072.963 kb on - strand at 1072.963 kb on - strand at 1073.159 kb on + strand, within yerC at 1073.248 kb on + strand, within yerC at 1073.248 kb on + strand, within yerC at 1073.249 kb on - strand, within yerC at 1073.249 kb on - strand, within yerC at 1073.272 kb on + strand, within yerC at 1073.272 kb on + strand, within yerC at 1073.273 kb on - strand, within yerC at 1073.671 kb on - strand, within hisG at 1073.671 kb on - strand, within hisG at 1073.671 kb on - strand, within hisG at 1073.716 kb on - strand, within hisG at 1073.716 kb on - strand, within hisG at 1073.849 kb on + strand, within hisG at 1074.415 kb on + strand, within hisD at 1074.415 kb on + strand, within hisD at 1074.415 kb on + strand, within hisD at 1074.415 kb on + strand, within hisD at 1074.415 kb on + strand, within hisD at 1074.533 kb on + strand, within hisD at 1074.533 kb on + strand, within hisD at 1074.533 kb on + strand, within hisD at 1074.534 kb on - strand, within hisD at 1074.534 kb on - strand, within hisD at 1074.534 kb on - strand, within hisD at 1074.534 kb on - strand, within hisD at 1074.534 kb on - strand, within hisD at 1074.534 kb on - strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.632 kb on - strand, within hisD at 1074.632 kb on - strand, within hisD at 1074.632 kb on - strand, within hisD at 1074.632 kb on - strand, within hisD at 1074.632 kb on - strand, within hisD at 1074.632 kb on - strand, within hisD at 1074.632 kb on - strand, within hisD at 1074.632 kb on - strand, within hisD at 1074.632 kb on - strand, within hisD at 1074.632 kb on - strand, within hisD at 1074.632 kb on - strand, within hisD at 1074.632 kb on - strand, within hisD at 1074.766 kb on + strand, within hisD at 1074.766 kb on + strand, within hisD at 1074.766 kb on + strand, within hisD at 1074.767 kb on - strand, within hisD at 1074.767 kb on - strand, within hisD at 1074.767 kb on - strand, within hisD at 1074.767 kb on - strand, within hisD at 1074.767 kb on - strand, within hisD at 1074.767 kb on - strand, within hisD at 1074.767 kb on - strand, within hisD at 1074.767 kb on - strand, within hisD at 1074.838 kb on + strand, within hisD at 1074.839 kb on - strand, within hisD at 1074.839 kb on - strand, within hisD at 1074.839 kb on - strand, within hisD at 1074.839 kb on - strand, within hisD
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM remove 1,072,354 + OKGIIK_04935 0.65 +0.3 1,072,354 + OKGIIK_04935 0.65 +0.8 1,072,354 + OKGIIK_04935 0.65 +0.4 1,072,355 - OKGIIK_04935 0.65 -0.2 1,072,369 + OKGIIK_04935 0.66 -1.5 1,072,369 + OKGIIK_04935 0.66 +0.3 1,072,369 + OKGIIK_04935 0.66 +1.8 1,072,369 + OKGIIK_04935 0.66 +0.4 1,072,370 - OKGIIK_04935 0.66 +0.8 1,072,370 - OKGIIK_04935 0.66 -0.9 1,072,370 - OKGIIK_04935 0.66 +2.1 1,072,370 - OKGIIK_04935 0.66 +1.2 1,072,433 - OKGIIK_04935 0.73 +0.7 1,072,457 + OKGIIK_04935 0.75 -0.3 1,072,492 + OKGIIK_04935 0.79 -0.2 1,072,492 + OKGIIK_04935 0.79 -0.3 1,072,492 + OKGIIK_04935 0.79 -1.0 1,072,492 + OKGIIK_04935 0.79 +0.3 1,072,492 + OKGIIK_04935 0.79 +1.3 1,072,492 + OKGIIK_04935 0.79 -0.8 1,072,492 + OKGIIK_04935 0.79 +0.2 1,072,493 - OKGIIK_04935 0.79 +0.5 1,072,493 - OKGIIK_04935 0.79 -0.8 1,072,493 - OKGIIK_04935 0.79 -1.8 1,072,493 - OKGIIK_04935 0.79 +1.4 1,072,493 - OKGIIK_04935 0.79 -0.2 1,072,493 - OKGIIK_04935 0.79 +1.4 1,072,493 - OKGIIK_04935 0.79 -0.2 1,072,493 - OKGIIK_04935 0.79 -0.1 1,072,493 - OKGIIK_04935 0.79 -0.4 1,072,493 - OKGIIK_04935 0.79 -0.6 1,072,493 - OKGIIK_04935 0.79 -2.8 1,072,493 - OKGIIK_04935 0.79 +1.1 1,072,493 - OKGIIK_04935 0.79 -1.2 1,072,493 - OKGIIK_04935 0.79 +1.8 1,072,493 - OKGIIK_04935 0.79 -0.2 1,072,493 - OKGIIK_04935 0.79 +0.3 1,072,534 + OKGIIK_04935 0.83 -0.9 1,072,534 + OKGIIK_04935 0.83 -0.0 1,072,534 + OKGIIK_04935 0.83 -0.2 1,072,534 + OKGIIK_04935 0.83 -2.3 1,072,535 - OKGIIK_04935 0.83 -2.9 1,072,535 - OKGIIK_04935 0.83 -0.5 1,072,535 - OKGIIK_04935 0.83 +0.5 1,072,535 - OKGIIK_04935 0.83 -2.0 1,072,690 + +1.6 1,072,690 + -1.0 1,072,690 + +0.4 1,072,691 - +0.3 1,072,691 - +0.8 1,072,691 - -0.8 1,072,691 - -2.0 1,072,691 - +0.8 1,072,691 - +0.4 1,072,691 - +0.8 1,072,691 - +0.3 1,072,691 - -1.7 1,072,691 - -0.1 1,072,691 - +1.0 1,072,691 - +1.3 1,072,835 + -1.8 1,072,835 + -1.2 1,072,836 - -1.7 1,072,912 + +1.6 1,072,912 + -2.0 1,072,912 + +0.3 1,072,912 + -0.2 1,072,912 + -1.8 1,072,912 + -0.4 1,072,913 - +0.3 1,072,913 - +0.5 1,072,913 - -1.5 1,072,913 - +0.1 1,072,913 - +0.7 1,072,913 - +0.3 1,072,962 + +0.7 1,072,962 + +1.1 1,072,962 + -1.5 1,072,962 + +2.1 1,072,962 + +0.5 1,072,962 + -0.8 1,072,962 + -0.4 1,072,963 - -2.0 1,072,963 - +0.1 1,072,963 - -0.8 1,072,963 - -1.8 1,072,963 - -1.2 1,073,159 + yerC OKGIIK_04940 0.48 -0.2 1,073,248 + yerC OKGIIK_04940 0.74 +1.4 1,073,248 + yerC OKGIIK_04940 0.74 +2.2 1,073,249 - yerC OKGIIK_04940 0.74 -2.3 1,073,249 - yerC OKGIIK_04940 0.74 -0.2 1,073,272 + yerC OKGIIK_04940 0.81 +4.0 1,073,272 + yerC OKGIIK_04940 0.81 +2.5 1,073,273 - yerC OKGIIK_04940 0.82 -2.9 1,073,671 - hisG OKGIIK_04945 0.38 -0.8 1,073,671 - hisG OKGIIK_04945 0.38 -1.8 1,073,671 - hisG OKGIIK_04945 0.38 -1.5 1,073,716 - hisG OKGIIK_04945 0.43 -0.8 1,073,716 - hisG OKGIIK_04945 0.43 -0.8 1,073,849 + hisG OKGIIK_04945 0.58 -0.8 1,074,415 + hisD OKGIIK_04950 0.12 -2.2 1,074,415 + hisD OKGIIK_04950 0.12 -2.6 1,074,415 + hisD OKGIIK_04950 0.12 -0.4 1,074,415 + hisD OKGIIK_04950 0.12 +0.8 1,074,415 + hisD OKGIIK_04950 0.12 -2.8 1,074,533 + hisD OKGIIK_04950 0.22 -1.8 1,074,533 + hisD OKGIIK_04950 0.22 -3.5 1,074,533 + hisD OKGIIK_04950 0.22 -1.5 1,074,534 - hisD OKGIIK_04950 0.22 -1.8 1,074,534 - hisD OKGIIK_04950 0.22 -2.3 1,074,534 - hisD OKGIIK_04950 0.22 -1.2 1,074,534 - hisD OKGIIK_04950 0.22 -3.1 1,074,534 - hisD OKGIIK_04950 0.22 -2.9 1,074,534 - hisD OKGIIK_04950 0.22 -2.5 1,074,631 + hisD OKGIIK_04950 0.29 -1.5 1,074,631 + hisD OKGIIK_04950 0.29 -4.2 1,074,631 + hisD OKGIIK_04950 0.29 -0.8 1,074,631 + hisD OKGIIK_04950 0.29 -3.0 1,074,631 + hisD OKGIIK_04950 0.29 -0.8 1,074,631 + hisD OKGIIK_04950 0.29 -2.2 1,074,631 + hisD OKGIIK_04950 0.29 +0.0 1,074,631 + hisD OKGIIK_04950 0.29 -2.6 1,074,631 + hisD OKGIIK_04950 0.29 -1.5 1,074,631 + hisD OKGIIK_04950 0.29 -1.2 1,074,631 + hisD OKGIIK_04950 0.29 -2.6 1,074,631 + hisD OKGIIK_04950 0.29 -1.2 1,074,631 + hisD OKGIIK_04950 0.29 -2.3 1,074,632 - hisD OKGIIK_04950 0.29 -1.5 1,074,632 - hisD OKGIIK_04950 0.29 -1.8 1,074,632 - hisD OKGIIK_04950 0.29 -2.8 1,074,632 - hisD OKGIIK_04950 0.29 -0.2 1,074,632 - hisD OKGIIK_04950 0.29 -1.5 1,074,632 - hisD OKGIIK_04950 0.29 -0.8 1,074,632 - hisD OKGIIK_04950 0.29 -2.0 1,074,632 - hisD OKGIIK_04950 0.29 -0.2 1,074,632 - hisD OKGIIK_04950 0.29 -3.3 1,074,632 - hisD OKGIIK_04950 0.29 -1.2 1,074,632 - hisD OKGIIK_04950 0.29 -1.5 1,074,632 - hisD OKGIIK_04950 0.29 -1.8 1,074,766 + hisD OKGIIK_04950 0.39 -2.6 1,074,766 + hisD OKGIIK_04950 0.39 -2.3 1,074,766 + hisD OKGIIK_04950 0.39 -1.5 1,074,767 - hisD OKGIIK_04950 0.40 -1.8 1,074,767 - hisD OKGIIK_04950 0.40 -3.2 1,074,767 - hisD OKGIIK_04950 0.40 -0.8 1,074,767 - hisD OKGIIK_04950 0.40 -3.1 1,074,767 - hisD OKGIIK_04950 0.40 -0.2 1,074,767 - hisD OKGIIK_04950 0.40 -3.0 1,074,767 - hisD OKGIIK_04950 0.40 +0.2 1,074,767 - hisD OKGIIK_04950 0.40 -2.2 1,074,838 + hisD OKGIIK_04950 0.45 -3.3 1,074,839 - hisD OKGIIK_04950 0.45 -2.2 1,074,839 - hisD OKGIIK_04950 0.45 -1.2 1,074,839 - hisD OKGIIK_04950 0.45 -1.5 1,074,839 - hisD OKGIIK_04950 0.45 -2.8
Or see this region's nucleotide sequence