Experiment: NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt minE and minD are separated by 5 nucleotides minD and minC are separated by 37 nucleotides minC and OKGIIK_03315 overlap by 4 nucleotides OKGIIK_03315 and OKGIIK_03320 overlap by 4 nucleotides
OKGIIK_03300: minE - cell division topological specificity factor MinE, at 742,633 to 742,929
minE
OKGIIK_03305: minD - septum site-determining protein MinD, at 742,935 to 743,750
minD
OKGIIK_03310: minC - septum site-determining protein MinC, at 743,788 to 744,564
minC
OKGIIK_03315: OKGIIK_03315 - hypothetical protein, at 744,561 to 744,824
_03315
OKGIIK_03320: OKGIIK_03320 - GNAT family N-acetyltransferase, at 744,821 to 745,441
_03320
Position (kb) 743
744
745 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 743.032 kb on + strand, within minD at 743.033 kb on - strand, within minD at 743.200 kb on + strand, within minD at 743.348 kb on - strand, within minD at 743.348 kb on - strand, within minD at 743.402 kb on - strand, within minD at 743.736 kb on - strand at 743.781 kb on + strand at 743.781 kb on + strand at 743.781 kb on + strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.790 kb on - strand at 743.790 kb on - strand at 743.985 kb on - strand, within minC at 744.065 kb on + strand, within minC at 744.066 kb on - strand, within minC at 744.066 kb on - strand, within minC at 744.066 kb on - strand, within minC at 744.224 kb on + strand, within minC at 744.565 kb on - strand at 744.565 kb on - strand at 744.625 kb on + strand, within OKGIIK_03315 at 744.625 kb on + strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.634 kb on - strand, within OKGIIK_03315 at 744.634 kb on - strand, within OKGIIK_03315 at 744.634 kb on - strand, within OKGIIK_03315 at 744.634 kb on - strand, within OKGIIK_03315 at 744.634 kb on - strand, within OKGIIK_03315 at 744.760 kb on - strand, within OKGIIK_03315 at 744.760 kb on - strand, within OKGIIK_03315 at 744.760 kb on - strand, within OKGIIK_03315 at 744.794 kb on - strand, within OKGIIK_03315 at 744.794 kb on - strand, within OKGIIK_03315 at 744.794 kb on - strand, within OKGIIK_03315 at 744.803 kb on - strand at 744.949 kb on - strand, within OKGIIK_03320 at 744.949 kb on - strand, within OKGIIK_03320 at 744.949 kb on - strand, within OKGIIK_03320 at 745.029 kb on + strand, within OKGIIK_03320 at 745.029 kb on + strand, within OKGIIK_03320 at 745.030 kb on - strand, within OKGIIK_03320 at 745.030 kb on - strand, within OKGIIK_03320 at 745.030 kb on - strand, within OKGIIK_03320 at 745.030 kb on - strand, within OKGIIK_03320 at 745.114 kb on - strand, within OKGIIK_03320 at 745.114 kb on - strand, within OKGIIK_03320 at 745.132 kb on - strand, within OKGIIK_03320 at 745.207 kb on - strand, within OKGIIK_03320 at 745.228 kb on - strand, within OKGIIK_03320 at 745.311 kb on + strand, within OKGIIK_03320 at 745.326 kb on + strand, within OKGIIK_03320 at 745.327 kb on - strand, within OKGIIK_03320 at 745.327 kb on - strand, within OKGIIK_03320 at 745.332 kb on + strand, within OKGIIK_03320 at 745.333 kb on - strand, within OKGIIK_03320 at 745.395 kb on - strand at 745.395 kb on - strand at 745.504 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.548 kb on + strand at 745.548 kb on + strand at 745.548 kb on + strand at 745.548 kb on + strand at 745.548 kb on + strand at 745.549 kb on - strand at 745.549 kb on - strand at 745.560 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM remove 743,032 + minD OKGIIK_03305 0.12 +3.2 743,033 - minD OKGIIK_03305 0.12 -1.7 743,200 + minD OKGIIK_03305 0.32 +0.1 743,348 - minD OKGIIK_03305 0.51 -0.7 743,348 - minD OKGIIK_03305 0.51 -2.3 743,402 - minD OKGIIK_03305 0.57 -2.1 743,736 - -1.4 743,781 + -0.7 743,781 + -2.4 743,781 + +0.5 743,782 - +1.9 743,782 - -0.5 743,782 - +0.2 743,782 - -0.5 743,782 - -1.1 743,782 - -0.1 743,782 - +0.1 743,782 - +0.3 743,782 - +1.5 743,782 - +1.5 743,782 - -1.2 743,782 - -0.5 743,782 - +0.9 743,782 - +0.1 743,782 - -0.6 743,782 - +1.7 743,782 - +0.1 743,782 - -0.7 743,790 - +0.7 743,790 - -1.1 743,985 - minC OKGIIK_03310 0.25 -0.9 744,065 + minC OKGIIK_03310 0.36 -2.3 744,066 - minC OKGIIK_03310 0.36 -1.5 744,066 - minC OKGIIK_03310 0.36 -0.7 744,066 - minC OKGIIK_03310 0.36 -0.1 744,224 + minC OKGIIK_03310 0.56 +1.5 744,565 - -2.6 744,565 - +1.6 744,625 + OKGIIK_03315 0.24 +1.5 744,625 + OKGIIK_03315 0.24 +0.4 744,626 - OKGIIK_03315 0.25 +1.1 744,626 - OKGIIK_03315 0.25 -1.0 744,626 - OKGIIK_03315 0.25 +1.0 744,626 - OKGIIK_03315 0.25 +2.7 744,626 - OKGIIK_03315 0.25 +0.1 744,626 - OKGIIK_03315 0.25 -0.7 744,626 - OKGIIK_03315 0.25 +1.6 744,626 - OKGIIK_03315 0.25 +0.9 744,626 - OKGIIK_03315 0.25 -1.9 744,626 - OKGIIK_03315 0.25 +0.7 744,626 - OKGIIK_03315 0.25 +1.9 744,626 - OKGIIK_03315 0.25 -0.6 744,626 - OKGIIK_03315 0.25 +0.9 744,626 - OKGIIK_03315 0.25 -1.1 744,626 - OKGIIK_03315 0.25 -0.3 744,626 - OKGIIK_03315 0.25 +0.5 744,626 - OKGIIK_03315 0.25 -1.5 744,626 - OKGIIK_03315 0.25 -2.5 744,626 - OKGIIK_03315 0.25 -0.6 744,626 - OKGIIK_03315 0.25 -2.3 744,626 - OKGIIK_03315 0.25 -0.5 744,626 - OKGIIK_03315 0.25 -1.7 744,626 - OKGIIK_03315 0.25 -0.7 744,626 - OKGIIK_03315 0.25 +0.5 744,626 - OKGIIK_03315 0.25 -0.3 744,626 - OKGIIK_03315 0.25 +1.9 744,626 - OKGIIK_03315 0.25 -0.6 744,626 - OKGIIK_03315 0.25 -2.3 744,634 - OKGIIK_03315 0.28 +0.9 744,634 - OKGIIK_03315 0.28 -3.2 744,634 - OKGIIK_03315 0.28 +0.1 744,634 - OKGIIK_03315 0.28 -0.1 744,634 - OKGIIK_03315 0.28 -1.1 744,760 - OKGIIK_03315 0.75 -0.4 744,760 - OKGIIK_03315 0.75 +0.4 744,760 - OKGIIK_03315 0.75 +0.3 744,794 - OKGIIK_03315 0.88 -0.5 744,794 - OKGIIK_03315 0.88 +0.1 744,794 - OKGIIK_03315 0.88 +1.9 744,803 - +0.9 744,949 - OKGIIK_03320 0.21 -1.5 744,949 - OKGIIK_03320 0.21 +0.6 744,949 - OKGIIK_03320 0.21 -1.1 745,029 + OKGIIK_03320 0.33 -0.1 745,029 + OKGIIK_03320 0.33 -0.6 745,030 - OKGIIK_03320 0.34 -1.5 745,030 - OKGIIK_03320 0.34 +1.2 745,030 - OKGIIK_03320 0.34 +0.3 745,030 - OKGIIK_03320 0.34 +1.9 745,114 - OKGIIK_03320 0.47 -0.7 745,114 - OKGIIK_03320 0.47 -2.1 745,132 - OKGIIK_03320 0.50 +1.4 745,207 - OKGIIK_03320 0.62 -1.7 745,228 - OKGIIK_03320 0.66 -1.1 745,311 + OKGIIK_03320 0.79 +1.9 745,326 + OKGIIK_03320 0.81 -0.1 745,327 - OKGIIK_03320 0.81 -0.5 745,327 - OKGIIK_03320 0.81 -1.9 745,332 + OKGIIK_03320 0.82 +0.9 745,333 - OKGIIK_03320 0.82 +0.5 745,395 - +0.1 745,395 - -2.1 745,504 + -1.1 745,527 + -0.1 745,527 + +0.5 745,527 + +0.1 745,527 + +0.9 745,527 + +0.9 745,527 + +0.9 745,527 + +0.6 745,527 + -0.1 745,527 + -0.4 745,527 + -0.2 745,527 + +0.9 745,527 + -0.2 745,527 + -0.1 745,527 + +1.1 745,527 + -1.7 745,527 + +0.2 745,527 + -1.5 745,527 + +1.6 745,527 + -0.4 745,527 + -0.1 745,527 + -1.5 745,527 + -0.1 745,527 + -1.1 745,527 + +1.9 745,528 - +1.2 745,528 - +0.9 745,528 - -0.1 745,528 - +2.1 745,528 - -0.1 745,528 - +1.9 745,528 - +0.6 745,528 - +1.3 745,528 - +2.5 745,528 - -1.1 745,528 - -0.1 745,528 - +0.6 745,528 - +0.9 745,528 - +0.1 745,528 - +0.5 745,528 - +0.3 745,528 - +2.5 745,548 + -1.7 745,548 + -0.1 745,548 + +0.5 745,548 + -1.6 745,548 + -1.1 745,549 - +0.9 745,549 - +2.2 745,560 - +1.9
Or see this region's nucleotide sequence