Experiment: NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_01770 and OKGIIK_01775 are separated by 205 nucleotides OKGIIK_01775 and OKGIIK_01780 are separated by 65 nucleotides OKGIIK_01780 and OKGIIK_01785 are separated by 106 nucleotides
OKGIIK_01770: OKGIIK_01770 - Transcriptional regulator ArgP, at 409,908 to 410,813
_01770
OKGIIK_01775: OKGIIK_01775 - L-lysine exporter family protein LysE/ArgO, at 411,019 to 411,636
_01775
OKGIIK_01780: OKGIIK_01780 - NAD+ synthase, at 411,702 to 413,387
_01780
OKGIIK_01785: OKGIIK_01785 - Outer membrane protein assembly factor BamD, at 413,494 to 414,360
_01785
Position (kb)
411
412
413
414 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 410.932 kb on + strand at 410.951 kb on + strand at 410.951 kb on + strand at 410.951 kb on + strand at 410.951 kb on + strand at 410.951 kb on + strand at 410.952 kb on - strand at 410.952 kb on - strand at 410.957 kb on + strand at 410.958 kb on - strand at 410.958 kb on - strand at 410.961 kb on + strand at 410.962 kb on - strand at 410.962 kb on - strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.025 kb on - strand at 411.025 kb on - strand at 411.025 kb on - strand at 411.025 kb on - strand at 411.025 kb on - strand at 411.025 kb on - strand at 411.025 kb on - strand at 411.034 kb on + strand at 411.034 kb on + strand at 411.034 kb on + strand at 411.034 kb on + strand at 411.035 kb on - strand at 411.035 kb on - strand at 411.035 kb on - strand at 411.035 kb on - strand at 411.076 kb on - strand at 411.076 kb on - strand at 411.076 kb on - strand at 411.241 kb on + strand, within OKGIIK_01775 at 411.241 kb on + strand, within OKGIIK_01775 at 411.242 kb on - strand, within OKGIIK_01775 at 411.242 kb on - strand, within OKGIIK_01775 at 411.242 kb on - strand, within OKGIIK_01775 at 411.242 kb on - strand, within OKGIIK_01775 at 411.242 kb on - strand, within OKGIIK_01775 at 411.269 kb on - strand, within OKGIIK_01775 at 411.269 kb on - strand, within OKGIIK_01775 at 411.395 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.439 kb on + strand, within OKGIIK_01775 at 411.439 kb on + strand, within OKGIIK_01775 at 411.439 kb on + strand, within OKGIIK_01775 at 411.440 kb on - strand, within OKGIIK_01775 at 411.440 kb on - strand, within OKGIIK_01775 at 411.514 kb on + strand, within OKGIIK_01775 at 411.514 kb on + strand, within OKGIIK_01775 at 411.514 kb on + strand, within OKGIIK_01775 at 411.514 kb on + strand, within OKGIIK_01775 at 411.514 kb on + strand, within OKGIIK_01775 at 411.515 kb on - strand, within OKGIIK_01775 at 411.515 kb on - strand, within OKGIIK_01775 at 411.515 kb on - strand, within OKGIIK_01775 at 411.515 kb on - strand, within OKGIIK_01775 at 411.515 kb on - strand, within OKGIIK_01775 at 411.665 kb on - strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.670 kb on - strand at 411.670 kb on - strand at 411.670 kb on - strand at 411.670 kb on - strand at 411.677 kb on + strand at 411.677 kb on + strand at 411.678 kb on - strand at 411.707 kb on + strand at 411.713 kb on - strand at 411.713 kb on - strand at 411.850 kb on - strand at 412.047 kb on + strand, within OKGIIK_01780 at 412.297 kb on - strand, within OKGIIK_01780 at 412.297 kb on - strand, within OKGIIK_01780 at 412.332 kb on + strand, within OKGIIK_01780 at 412.332 kb on + strand, within OKGIIK_01780 at 412.333 kb on - strand, within OKGIIK_01780 at 412.494 kb on + strand, within OKGIIK_01780 at 412.494 kb on + strand, within OKGIIK_01780 at 412.494 kb on + strand, within OKGIIK_01780 at 412.495 kb on - strand, within OKGIIK_01780 at 412.495 kb on - strand, within OKGIIK_01780 at 412.495 kb on - strand, within OKGIIK_01780 at 412.524 kb on + strand, within OKGIIK_01780 at 412.524 kb on + strand, within OKGIIK_01780 at 412.524 kb on + strand, within OKGIIK_01780 at 412.524 kb on + strand, within OKGIIK_01780 at 412.524 kb on + strand, within OKGIIK_01780 at 412.525 kb on - strand, within OKGIIK_01780 at 412.525 kb on - strand, within OKGIIK_01780 at 412.525 kb on - strand, within OKGIIK_01780 at 412.525 kb on - strand, within OKGIIK_01780 at 412.656 kb on + strand, within OKGIIK_01780 at 412.657 kb on - strand, within OKGIIK_01780 at 412.657 kb on - strand, within OKGIIK_01780 at 412.716 kb on + strand, within OKGIIK_01780 at 412.912 kb on - strand, within OKGIIK_01780 at 412.923 kb on + strand, within OKGIIK_01780 at 412.945 kb on - strand, within OKGIIK_01780 at 412.945 kb on - strand, within OKGIIK_01780 at 412.965 kb on + strand, within OKGIIK_01780 at 412.966 kb on - strand, within OKGIIK_01780 at 412.966 kb on - strand, within OKGIIK_01780 at 412.966 kb on - strand, within OKGIIK_01780 at 412.966 kb on - strand, within OKGIIK_01780 at 413.169 kb on + strand, within OKGIIK_01780 at 413.369 kb on + strand at 413.369 kb on + strand at 413.369 kb on + strand at 413.370 kb on - strand at 413.370 kb on - strand at 413.370 kb on - strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined_nocarbon with D-Glucose and L-Methionine 1 mM remove 410,932 + +0.3 410,951 + -0.4 410,951 + +0.7 410,951 + -1.7 410,951 + +1.5 410,951 + +0.4 410,952 - +0.2 410,952 - -2.2 410,957 + +1.0 410,958 - -0.3 410,958 - +1.7 410,961 + -1.0 410,962 - -1.5 410,962 - -0.0 411,024 + +0.1 411,024 + +0.5 411,024 + +0.3 411,024 + -0.2 411,024 + -1.4 411,024 + +0.5 411,024 + +0.2 411,024 + +0.2 411,024 + +0.3 411,024 + -0.8 411,024 + +1.6 411,025 - -2.8 411,025 - -1.1 411,025 - -0.0 411,025 - +0.8 411,025 - -2.2 411,025 - +1.0 411,025 - -0.0 411,034 + -1.8 411,034 + -1.2 411,034 + -0.2 411,034 + -0.1 411,035 - +0.4 411,035 - +1.0 411,035 - -0.4 411,035 - +0.4 411,076 - -2.0 411,076 - -0.2 411,076 - -1.0 411,241 + OKGIIK_01775 0.36 -0.0 411,241 + OKGIIK_01775 0.36 -0.0 411,242 - OKGIIK_01775 0.36 -1.0 411,242 - OKGIIK_01775 0.36 +0.6 411,242 - OKGIIK_01775 0.36 -0.2 411,242 - OKGIIK_01775 0.36 -0.3 411,242 - OKGIIK_01775 0.36 +0.4 411,269 - OKGIIK_01775 0.40 -0.5 411,269 - OKGIIK_01775 0.40 -1.0 411,395 + OKGIIK_01775 0.61 -1.4 411,397 + OKGIIK_01775 0.61 -1.0 411,397 + OKGIIK_01775 0.61 +2.0 411,397 + OKGIIK_01775 0.61 +0.7 411,397 + OKGIIK_01775 0.61 -1.6 411,397 + OKGIIK_01775 0.61 -1.4 411,397 + OKGIIK_01775 0.61 +1.8 411,397 + OKGIIK_01775 0.61 -1.8 411,397 + OKGIIK_01775 0.61 +0.4 411,397 + OKGIIK_01775 0.61 +0.4 411,397 + OKGIIK_01775 0.61 -1.4 411,397 + OKGIIK_01775 0.61 +2.6 411,398 - OKGIIK_01775 0.61 +0.2 411,398 - OKGIIK_01775 0.61 -0.0 411,398 - OKGIIK_01775 0.61 +1.0 411,398 - OKGIIK_01775 0.61 -1.8 411,398 - OKGIIK_01775 0.61 +2.4 411,398 - OKGIIK_01775 0.61 -0.6 411,398 - OKGIIK_01775 0.61 +2.0 411,398 - OKGIIK_01775 0.61 +0.3 411,398 - OKGIIK_01775 0.61 -0.0 411,398 - OKGIIK_01775 0.61 +1.2 411,439 + OKGIIK_01775 0.68 -0.8 411,439 + OKGIIK_01775 0.68 +2.6 411,439 + OKGIIK_01775 0.68 -0.6 411,440 - OKGIIK_01775 0.68 -2.0 411,440 - OKGIIK_01775 0.68 -0.0 411,514 + OKGIIK_01775 0.80 +1.0 411,514 + OKGIIK_01775 0.80 -0.2 411,514 + OKGIIK_01775 0.80 -0.2 411,514 + OKGIIK_01775 0.80 -0.8 411,514 + OKGIIK_01775 0.80 -2.0 411,515 - OKGIIK_01775 0.80 +1.2 411,515 - OKGIIK_01775 0.80 -0.6 411,515 - OKGIIK_01775 0.80 -0.6 411,515 - OKGIIK_01775 0.80 +0.4 411,515 - OKGIIK_01775 0.80 -2.0 411,665 - -0.4 411,669 + +0.2 411,669 + -1.0 411,669 + -0.6 411,669 + -1.3 411,669 + -0.0 411,669 + +1.0 411,669 + -0.2 411,669 + -0.0 411,669 + -0.6 411,669 + -2.0 411,669 + +0.0 411,669 + +1.0 411,669 + -0.5 411,669 + -2.4 411,669 + -0.8 411,669 + -0.3 411,669 + -0.3 411,670 - -0.3 411,670 - -0.6 411,670 - -2.7 411,670 - -2.4 411,677 + -1.7 411,677 + -0.6 411,678 - +0.4 411,707 + -0.3 411,713 - +1.0 411,713 - +0.7 411,850 - -2.4 412,047 + OKGIIK_01780 0.20 -2.0 412,297 - OKGIIK_01780 0.35 -2.0 412,297 - OKGIIK_01780 0.35 -0.6 412,332 + OKGIIK_01780 0.37 -2.4 412,332 + OKGIIK_01780 0.37 -2.0 412,333 - OKGIIK_01780 0.37 -2.5 412,494 + OKGIIK_01780 0.47 -2.0 412,494 + OKGIIK_01780 0.47 -2.5 412,494 + OKGIIK_01780 0.47 -1.0 412,495 - OKGIIK_01780 0.47 -2.2 412,495 - OKGIIK_01780 0.47 -1.0 412,495 - OKGIIK_01780 0.47 -1.0 412,524 + OKGIIK_01780 0.49 -1.7 412,524 + OKGIIK_01780 0.49 -0.4 412,524 + OKGIIK_01780 0.49 -1.0 412,524 + OKGIIK_01780 0.49 -1.0 412,524 + OKGIIK_01780 0.49 -2.6 412,525 - OKGIIK_01780 0.49 -1.8 412,525 - OKGIIK_01780 0.49 -2.2 412,525 - OKGIIK_01780 0.49 -3.8 412,525 - OKGIIK_01780 0.49 -2.0 412,656 + OKGIIK_01780 0.57 -2.5 412,657 - OKGIIK_01780 0.57 -0.0 412,657 - OKGIIK_01780 0.57 -2.5 412,716 + OKGIIK_01780 0.60 -1.8 412,912 - OKGIIK_01780 0.72 -3.1 412,923 + OKGIIK_01780 0.72 -1.4 412,945 - OKGIIK_01780 0.74 -3.2 412,945 - OKGIIK_01780 0.74 -0.6 412,965 + OKGIIK_01780 0.75 -1.8 412,966 - OKGIIK_01780 0.75 +1.0 412,966 - OKGIIK_01780 0.75 -0.8 412,966 - OKGIIK_01780 0.75 -1.4 412,966 - OKGIIK_01780 0.75 -1.5 413,169 + OKGIIK_01780 0.87 -2.0 413,369 + -0.3 413,369 + +1.9 413,369 + +1.2 413,370 - -2.0 413,370 - -2.6 413,370 - -2.0 413,444 + +0.7 413,444 + -1.4 413,444 + +0.7 413,444 + +0.6 413,444 + -0.2 413,444 + +0.3 413,444 + -2.0 413,444 + -1.6 413,444 + -0.4 413,444 + -0.6 413,444 + -1.0 413,444 + -0.6 413,444 + +1.6 413,444 + +1.2 413,444 + +1.3 413,445 - +0.6 413,445 - +1.4 413,445 - -1.6 413,445 - -0.3 413,445 - +0.2 413,445 - -0.7 413,445 - +0.4 413,445 - +2.3 413,445 - -0.5 413,445 - +0.6 413,445 - -0.0 413,445 - +1.2 413,445 - +0.6 413,445 - +1.0 413,445 - -0.6 413,445 - +0.5 413,445 - -1.6 413,445 - -0.6 413,445 - +1.6
Or see this region's nucleotide sequence