Experiment: Survival; Treatment=20250415-M6-SZ1-UF; TreatmentDuration=24hrs; Outgrowth=R2A_Kan10_Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABCV34_RS04325 and ABCV34_RS04330 overlap by 17 nucleotides ABCV34_RS04330 and ABCV34_RS04335 are separated by 68 nucleotides
ABCV34_RS04325: ABCV34_RS04325 - YihY family inner membrane protein, at 944,409 to 945,716
_RS04325
ABCV34_RS04330: ABCV34_RS04330 - CBS domain-containing protein, at 945,700 to 946,167
_RS04330
ABCV34_RS04335: ABCV34_RS04335 - tRNA dihydrouridine(20/20a) synthase DusA, at 946,236 to 947,240
_RS04335
Position (kb) 945
946
947 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 944.844 kb on + strand, within ABCV34_RS04325 at 944.845 kb on - strand, within ABCV34_RS04325 at 944.845 kb on - strand, within ABCV34_RS04325 at 944.845 kb on - strand, within ABCV34_RS04325 at 944.845 kb on - strand, within ABCV34_RS04325 at 944.845 kb on - strand, within ABCV34_RS04325 at 944.845 kb on - strand, within ABCV34_RS04325 at 944.928 kb on + strand, within ABCV34_RS04325 at 944.928 kb on + strand, within ABCV34_RS04325 at 944.928 kb on + strand, within ABCV34_RS04325 at 944.928 kb on + strand, within ABCV34_RS04325 at 944.929 kb on - strand, within ABCV34_RS04325 at 944.929 kb on - strand, within ABCV34_RS04325 at 944.929 kb on - strand, within ABCV34_RS04325 at 944.929 kb on - strand, within ABCV34_RS04325 at 944.929 kb on - strand, within ABCV34_RS04325 at 944.929 kb on - strand, within ABCV34_RS04325 at 944.929 kb on - strand, within ABCV34_RS04325 at 944.941 kb on + strand, within ABCV34_RS04325 at 944.942 kb on - strand, within ABCV34_RS04325 at 945.114 kb on + strand, within ABCV34_RS04325 at 945.114 kb on + strand, within ABCV34_RS04325 at 945.114 kb on + strand, within ABCV34_RS04325 at 945.115 kb on - strand, within ABCV34_RS04325 at 945.115 kb on - strand, within ABCV34_RS04325 at 945.135 kb on + strand, within ABCV34_RS04325 at 945.135 kb on + strand, within ABCV34_RS04325 at 945.135 kb on + strand, within ABCV34_RS04325 at 945.135 kb on + strand, within ABCV34_RS04325 at 945.136 kb on - strand, within ABCV34_RS04325 at 945.136 kb on - strand, within ABCV34_RS04325 at 945.136 kb on - strand, within ABCV34_RS04325 at 945.136 kb on - strand, within ABCV34_RS04325 at 945.137 kb on + strand, within ABCV34_RS04325 at 945.137 kb on + strand, within ABCV34_RS04325 at 945.137 kb on + strand, within ABCV34_RS04325 at 945.138 kb on - strand, within ABCV34_RS04325 at 945.138 kb on - strand, within ABCV34_RS04325 at 945.147 kb on + strand, within ABCV34_RS04325 at 945.147 kb on + strand, within ABCV34_RS04325 at 945.148 kb on - strand, within ABCV34_RS04325 at 945.189 kb on + strand, within ABCV34_RS04325 at 945.189 kb on + strand, within ABCV34_RS04325 at 945.189 kb on + strand, within ABCV34_RS04325 at 945.189 kb on + strand, within ABCV34_RS04325 at 945.189 kb on + strand, within ABCV34_RS04325 at 945.189 kb on + strand, within ABCV34_RS04325 at 945.189 kb on + strand, within ABCV34_RS04325 at 945.189 kb on + strand, within ABCV34_RS04325 at 945.190 kb on - strand, within ABCV34_RS04325 at 945.190 kb on - strand, within ABCV34_RS04325 at 945.190 kb on - strand, within ABCV34_RS04325 at 945.344 kb on - strand, within ABCV34_RS04325 at 945.438 kb on + strand, within ABCV34_RS04325 at 945.439 kb on - strand, within ABCV34_RS04325 at 945.439 kb on - strand, within ABCV34_RS04325 at 945.586 kb on + strand at 945.660 kb on + strand at 945.660 kb on + strand at 945.661 kb on - strand at 945.661 kb on - strand at 945.661 kb on - strand at 945.745 kb on + strand at 945.746 kb on - strand at 945.746 kb on - strand at 945.746 kb on - strand at 945.746 kb on - strand at 945.763 kb on - strand, within ABCV34_RS04330 at 945.907 kb on + strand, within ABCV34_RS04330 at 945.907 kb on + strand, within ABCV34_RS04330 at 945.907 kb on + strand, within ABCV34_RS04330 at 945.907 kb on + strand, within ABCV34_RS04330 at 945.908 kb on - strand, within ABCV34_RS04330 at 945.908 kb on - strand, within ABCV34_RS04330 at 946.006 kb on + strand, within ABCV34_RS04330 at 946.006 kb on + strand, within ABCV34_RS04330 at 946.006 kb on + strand, within ABCV34_RS04330 at 946.006 kb on + strand, within ABCV34_RS04330 at 946.006 kb on + strand, within ABCV34_RS04330 at 946.006 kb on + strand, within ABCV34_RS04330 at 946.006 kb on + strand, within ABCV34_RS04330 at 946.006 kb on + strand, within ABCV34_RS04330 at 946.006 kb on + strand, within ABCV34_RS04330 at 946.006 kb on + strand, within ABCV34_RS04330 at 946.007 kb on - strand, within ABCV34_RS04330 at 946.007 kb on - strand, within ABCV34_RS04330 at 946.007 kb on - strand, within ABCV34_RS04330 at 946.007 kb on - strand, within ABCV34_RS04330 at 946.007 kb on - strand, within ABCV34_RS04330 at 946.007 kb on - strand, within ABCV34_RS04330 at 946.007 kb on - strand, within ABCV34_RS04330 at 946.054 kb on + strand, within ABCV34_RS04330 at 946.054 kb on + strand, within ABCV34_RS04330 at 946.054 kb on + strand, within ABCV34_RS04330 at 946.054 kb on + strand, within ABCV34_RS04330 at 946.054 kb on + strand, within ABCV34_RS04330 at 946.055 kb on - strand, within ABCV34_RS04330 at 946.055 kb on - strand, within ABCV34_RS04330 at 946.055 kb on - strand, within ABCV34_RS04330 at 946.055 kb on - strand, within ABCV34_RS04330 at 946.055 kb on - strand, within ABCV34_RS04330 at 946.102 kb on - strand, within ABCV34_RS04330 at 946.102 kb on - strand, within ABCV34_RS04330 at 946.114 kb on + strand, within ABCV34_RS04330 at 946.114 kb on + strand, within ABCV34_RS04330 at 946.114 kb on + strand, within ABCV34_RS04330 at 946.114 kb on + strand, within ABCV34_RS04330 at 946.114 kb on + strand, within ABCV34_RS04330 at 946.114 kb on + strand, within ABCV34_RS04330 at 946.114 kb on + strand, within ABCV34_RS04330 at 946.114 kb on + strand, within ABCV34_RS04330 at 946.114 kb on + strand, within ABCV34_RS04330 at 946.114 kb on + strand, within ABCV34_RS04330 at 946.114 kb on + strand, within ABCV34_RS04330 at 946.115 kb on - strand, within ABCV34_RS04330 at 946.115 kb on - strand, within ABCV34_RS04330 at 946.115 kb on - strand, within ABCV34_RS04330 at 946.115 kb on - strand, within ABCV34_RS04330 at 946.115 kb on - strand, within ABCV34_RS04330 at 946.115 kb on - strand, within ABCV34_RS04330 at 946.115 kb on - strand, within ABCV34_RS04330 at 946.115 kb on - strand, within ABCV34_RS04330 at 946.115 kb on - strand, within ABCV34_RS04330 at 946.735 kb on - strand, within ABCV34_RS04335 at 946.753 kb on + strand, within ABCV34_RS04335 at 946.753 kb on + strand, within ABCV34_RS04335 at 946.753 kb on + strand, within ABCV34_RS04335 at 946.753 kb on + strand, within ABCV34_RS04335 at 946.753 kb on + strand, within ABCV34_RS04335 at 946.754 kb on - strand, within ABCV34_RS04335 at 946.754 kb on - strand, within ABCV34_RS04335 at 946.754 kb on - strand, within ABCV34_RS04335 at 946.754 kb on - strand, within ABCV34_RS04335 at 946.787 kb on - strand, within ABCV34_RS04335 at 946.787 kb on - strand, within ABCV34_RS04335 at 946.798 kb on + strand, within ABCV34_RS04335 at 946.799 kb on - strand, within ABCV34_RS04335 at 947.116 kb on + strand, within ABCV34_RS04335 at 947.116 kb on + strand, within ABCV34_RS04335 at 947.116 kb on + strand, within ABCV34_RS04335 at 947.116 kb on + strand, within ABCV34_RS04335 at 947.116 kb on + strand, within ABCV34_RS04335 at 947.152 kb on + strand at 947.152 kb on + strand at 947.153 kb on - strand at 947.153 kb on - strand at 947.153 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=20250415-M6-SZ1-UF; TreatmentDuration=24hrs; Outgrowth=R2A_Kan10_Lactate remove 944,844 + ABCV34_RS04325 0.33 -1.7 944,845 - ABCV34_RS04325 0.33 -0.2 944,845 - ABCV34_RS04325 0.33 -0.6 944,845 - ABCV34_RS04325 0.33 -1.8 944,845 - ABCV34_RS04325 0.33 -0.7 944,845 - ABCV34_RS04325 0.33 -0.9 944,845 - ABCV34_RS04325 0.33 -1.0 944,928 + ABCV34_RS04325 0.40 +0.2 944,928 + ABCV34_RS04325 0.40 -2.4 944,928 + ABCV34_RS04325 0.40 +0.7 944,928 + ABCV34_RS04325 0.40 -1.9 944,929 - ABCV34_RS04325 0.40 -0.1 944,929 - ABCV34_RS04325 0.40 -1.1 944,929 - ABCV34_RS04325 0.40 +2.0 944,929 - ABCV34_RS04325 0.40 +0.1 944,929 - ABCV34_RS04325 0.40 -2.8 944,929 - ABCV34_RS04325 0.40 +0.8 944,929 - ABCV34_RS04325 0.40 -1.2 944,941 + ABCV34_RS04325 0.41 -1.2 944,942 - ABCV34_RS04325 0.41 -2.5 945,114 + ABCV34_RS04325 0.54 +0.2 945,114 + ABCV34_RS04325 0.54 -1.7 945,114 + ABCV34_RS04325 0.54 +1.1 945,115 - ABCV34_RS04325 0.54 +0.2 945,115 - ABCV34_RS04325 0.54 -1.4 945,135 + ABCV34_RS04325 0.56 -0.3 945,135 + ABCV34_RS04325 0.56 -2.7 945,135 + ABCV34_RS04325 0.56 -3.9 945,135 + ABCV34_RS04325 0.56 -2.5 945,136 - ABCV34_RS04325 0.56 -2.4 945,136 - ABCV34_RS04325 0.56 -3.2 945,136 - ABCV34_RS04325 0.56 -1.2 945,136 - ABCV34_RS04325 0.56 -0.9 945,137 + ABCV34_RS04325 0.56 +0.8 945,137 + ABCV34_RS04325 0.56 -1.2 945,137 + ABCV34_RS04325 0.56 -0.5 945,138 - ABCV34_RS04325 0.56 -2.1 945,138 - ABCV34_RS04325 0.56 -1.4 945,147 + ABCV34_RS04325 0.56 +0.3 945,147 + ABCV34_RS04325 0.56 -0.1 945,148 - ABCV34_RS04325 0.56 +0.4 945,189 + ABCV34_RS04325 0.60 -4.1 945,189 + ABCV34_RS04325 0.60 +1.0 945,189 + ABCV34_RS04325 0.60 -0.1 945,189 + ABCV34_RS04325 0.60 +0.1 945,189 + ABCV34_RS04325 0.60 -1.6 945,189 + ABCV34_RS04325 0.60 +0.5 945,189 + ABCV34_RS04325 0.60 -0.9 945,189 + ABCV34_RS04325 0.60 +1.0 945,190 - ABCV34_RS04325 0.60 +2.6 945,190 - ABCV34_RS04325 0.60 -2.9 945,190 - ABCV34_RS04325 0.60 -0.7 945,344 - ABCV34_RS04325 0.71 +0.3 945,438 + ABCV34_RS04325 0.79 -2.9 945,439 - ABCV34_RS04325 0.79 -2.5 945,439 - ABCV34_RS04325 0.79 +0.0 945,586 + -1.3 945,660 + -0.1 945,660 + -1.1 945,661 - +0.5 945,661 - -1.7 945,661 - -1.4 945,745 + -1.0 945,746 - -1.4 945,746 - -0.4 945,746 - -0.6 945,746 - -3.2 945,763 - ABCV34_RS04330 0.13 -1.1 945,907 + ABCV34_RS04330 0.44 -3.0 945,907 + ABCV34_RS04330 0.44 -0.9 945,907 + ABCV34_RS04330 0.44 +1.1 945,907 + ABCV34_RS04330 0.44 +0.6 945,908 - ABCV34_RS04330 0.44 +0.2 945,908 - ABCV34_RS04330 0.44 +0.1 946,006 + ABCV34_RS04330 0.65 -2.5 946,006 + ABCV34_RS04330 0.65 +0.1 946,006 + ABCV34_RS04330 0.65 +0.9 946,006 + ABCV34_RS04330 0.65 +1.1 946,006 + ABCV34_RS04330 0.65 +0.4 946,006 + ABCV34_RS04330 0.65 -0.3 946,006 + ABCV34_RS04330 0.65 +0.5 946,006 + ABCV34_RS04330 0.65 -1.6 946,006 + ABCV34_RS04330 0.65 -0.7 946,006 + ABCV34_RS04330 0.65 -0.9 946,007 - ABCV34_RS04330 0.66 +0.2 946,007 - ABCV34_RS04330 0.66 +0.2 946,007 - ABCV34_RS04330 0.66 +0.8 946,007 - ABCV34_RS04330 0.66 -1.7 946,007 - ABCV34_RS04330 0.66 -0.7 946,007 - ABCV34_RS04330 0.66 +0.4 946,007 - ABCV34_RS04330 0.66 -1.1 946,054 + ABCV34_RS04330 0.76 +0.0 946,054 + ABCV34_RS04330 0.76 -0.8 946,054 + ABCV34_RS04330 0.76 +1.1 946,054 + ABCV34_RS04330 0.76 -0.6 946,054 + ABCV34_RS04330 0.76 +0.8 946,055 - ABCV34_RS04330 0.76 -0.1 946,055 - ABCV34_RS04330 0.76 -0.1 946,055 - ABCV34_RS04330 0.76 -0.5 946,055 - ABCV34_RS04330 0.76 -0.6 946,055 - ABCV34_RS04330 0.76 +2.5 946,102 - ABCV34_RS04330 0.86 -1.1 946,102 - ABCV34_RS04330 0.86 -1.2 946,114 + ABCV34_RS04330 0.88 -1.7 946,114 + ABCV34_RS04330 0.88 -1.5 946,114 + ABCV34_RS04330 0.88 -0.7 946,114 + ABCV34_RS04330 0.88 +0.7 946,114 + ABCV34_RS04330 0.88 +0.7 946,114 + ABCV34_RS04330 0.88 -0.2 946,114 + ABCV34_RS04330 0.88 -1.1 946,114 + ABCV34_RS04330 0.88 +0.8 946,114 + ABCV34_RS04330 0.88 +0.3 946,114 + ABCV34_RS04330 0.88 -2.5 946,114 + ABCV34_RS04330 0.88 -0.3 946,115 - ABCV34_RS04330 0.89 -0.2 946,115 - ABCV34_RS04330 0.89 -1.6 946,115 - ABCV34_RS04330 0.89 -1.3 946,115 - ABCV34_RS04330 0.89 -1.1 946,115 - ABCV34_RS04330 0.89 -1.1 946,115 - ABCV34_RS04330 0.89 -1.5 946,115 - ABCV34_RS04330 0.89 -1.9 946,115 - ABCV34_RS04330 0.89 -0.6 946,115 - ABCV34_RS04330 0.89 -2.7 946,735 - ABCV34_RS04335 0.50 -0.9 946,753 + ABCV34_RS04335 0.51 -1.3 946,753 + ABCV34_RS04335 0.51 +0.2 946,753 + ABCV34_RS04335 0.51 -1.8 946,753 + ABCV34_RS04335 0.51 -1.2 946,753 + ABCV34_RS04335 0.51 -1.7 946,754 - ABCV34_RS04335 0.52 -1.4 946,754 - ABCV34_RS04335 0.52 -0.9 946,754 - ABCV34_RS04335 0.52 -0.8 946,754 - ABCV34_RS04335 0.52 -0.4 946,787 - ABCV34_RS04335 0.55 -3.1 946,787 - ABCV34_RS04335 0.55 -0.6 946,798 + ABCV34_RS04335 0.56 -2.5 946,799 - ABCV34_RS04335 0.56 +1.0 947,116 + ABCV34_RS04335 0.88 +2.1 947,116 + ABCV34_RS04335 0.88 -0.1 947,116 + ABCV34_RS04335 0.88 +0.7 947,116 + ABCV34_RS04335 0.88 -1.4 947,116 + ABCV34_RS04335 0.88 -0.5 947,152 + -2.9 947,152 + +0.1 947,153 - -0.5 947,153 - +0.3 947,153 - -1.3
Or see this region's nucleotide sequence