Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yjgN and argA are separated by 192 nucleotides argA and rraB are separated by 45 nucleotides rraB and argF are separated by 161 nucleotides
MCAODC_02325: yjgN - Inner membrane protein YjgN, at 477,794 to 478,990
yjgN
MCAODC_02330: argA - GNAT family N-acetyltransferase, at 479,183 to 479,686
argA
MCAODC_02335: rraB - ribonuclease E inhibitor RraB, at 479,732 to 480,148
rraB
MCAODC_02340: argF - ornithine carbamoyltransferase, at 480,310 to 481,314
argF
Position (kb) 479
480
481 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 479.028 kb on + strand at 479.079 kb on - strand at 479.125 kb on + strand at 479.183 kb on - strand at 479.279 kb on + strand, within argA at 479.279 kb on + strand, within argA at 479.279 kb on + strand, within argA at 479.280 kb on - strand, within argA at 479.296 kb on + strand, within argA at 479.345 kb on + strand, within argA at 479.345 kb on + strand, within argA at 479.345 kb on + strand, within argA at 479.346 kb on - strand, within argA at 479.346 kb on - strand, within argA at 479.346 kb on - strand, within argA at 479.346 kb on - strand, within argA at 479.346 kb on - strand, within argA at 479.366 kb on + strand, within argA at 479.366 kb on + strand, within argA at 479.366 kb on + strand, within argA at 479.381 kb on + strand, within argA at 479.421 kb on + strand, within argA at 479.422 kb on - strand, within argA at 479.476 kb on + strand, within argA at 479.511 kb on + strand, within argA at 479.608 kb on - strand, within argA at 479.608 kb on - strand, within argA at 479.620 kb on + strand, within argA at 479.620 kb on + strand, within argA at 479.674 kb on - strand at 479.729 kb on + strand at 479.729 kb on + strand at 479.730 kb on - strand at 479.730 kb on - strand at 479.808 kb on + strand, within rraB at 479.808 kb on + strand, within rraB at 479.809 kb on - strand, within rraB at 479.809 kb on - strand, within rraB at 479.809 kb on - strand, within rraB at 479.809 kb on - strand, within rraB at 480.055 kb on - strand, within rraB at 480.162 kb on + strand at 480.162 kb on + strand at 480.162 kb on + strand at 480.163 kb on - strand at 480.163 kb on - strand at 480.163 kb on - strand at 480.182 kb on + strand at 480.183 kb on - strand at 480.309 kb on + strand at 480.309 kb on + strand at 480.310 kb on - strand at 480.310 kb on - strand at 480.310 kb on - strand at 480.310 kb on - strand at 480.310 kb on - strand at 480.310 kb on - strand at 480.310 kb on - strand at 480.408 kb on + strand at 480.444 kb on + strand, within argF at 480.445 kb on - strand, within argF at 480.511 kb on + strand, within argF at 480.511 kb on + strand, within argF at 480.512 kb on - strand, within argF at 480.531 kb on + strand, within argF at 480.535 kb on + strand, within argF at 480.535 kb on + strand, within argF at 480.535 kb on + strand, within argF at 480.536 kb on - strand, within argF at 480.536 kb on - strand, within argF at 480.536 kb on - strand, within argF at 480.567 kb on + strand, within argF at 480.567 kb on + strand, within argF at 480.610 kb on + strand, within argF at 480.610 kb on + strand, within argF at 480.610 kb on + strand, within argF at 480.610 kb on + strand, within argF at 480.611 kb on - strand, within argF at 480.611 kb on - strand, within argF at 480.611 kb on - strand, within argF at 480.667 kb on + strand, within argF at 480.668 kb on - strand, within argF at 480.668 kb on - strand, within argF at 480.791 kb on - strand, within argF at 480.791 kb on - strand, within argF at 480.791 kb on - strand, within argF at 480.808 kb on - strand, within argF at 480.808 kb on - strand, within argF at 480.808 kb on - strand, within argF at 480.948 kb on + strand, within argF at 480.965 kb on + strand, within argF at 480.966 kb on - strand, within argF at 480.966 kb on - strand, within argF at 480.966 kb on - strand, within argF at 480.993 kb on + strand, within argF at 480.993 kb on + strand, within argF at 480.993 kb on + strand, within argF at 480.994 kb on - strand, within argF at 480.994 kb on - strand, within argF at 480.998 kb on - strand, within argF at 480.998 kb on - strand, within argF at 481.000 kb on - strand, within argF at 481.007 kb on + strand, within argF at 481.007 kb on + strand, within argF at 481.007 kb on + strand, within argF at 481.007 kb on + strand, within argF at 481.008 kb on - strand, within argF at 481.008 kb on - strand, within argF at 481.008 kb on - strand, within argF at 481.059 kb on - strand, within argF at 481.059 kb on - strand, within argF
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 479,028 + +0.2 479,079 - +1.1 479,125 + -0.6 479,183 - +0.4 479,279 + argA MCAODC_02330 0.19 +0.3 479,279 + argA MCAODC_02330 0.19 -0.4 479,279 + argA MCAODC_02330 0.19 +2.1 479,280 - argA MCAODC_02330 0.19 +0.1 479,296 + argA MCAODC_02330 0.22 -1.1 479,345 + argA MCAODC_02330 0.32 -0.1 479,345 + argA MCAODC_02330 0.32 -2.2 479,345 + argA MCAODC_02330 0.32 +0.1 479,346 - argA MCAODC_02330 0.32 +0.8 479,346 - argA MCAODC_02330 0.32 -1.0 479,346 - argA MCAODC_02330 0.32 +1.9 479,346 - argA MCAODC_02330 0.32 +0.6 479,346 - argA MCAODC_02330 0.32 -1.0 479,366 + argA MCAODC_02330 0.36 -1.2 479,366 + argA MCAODC_02330 0.36 +0.5 479,366 + argA MCAODC_02330 0.36 -0.8 479,381 + argA MCAODC_02330 0.39 -0.1 479,421 + argA MCAODC_02330 0.47 -0.8 479,422 - argA MCAODC_02330 0.47 +0.8 479,476 + argA MCAODC_02330 0.58 -0.6 479,511 + argA MCAODC_02330 0.65 +0.3 479,608 - argA MCAODC_02330 0.84 +0.6 479,608 - argA MCAODC_02330 0.84 -0.3 479,620 + argA MCAODC_02330 0.87 -0.2 479,620 + argA MCAODC_02330 0.87 -0.7 479,674 - +1.2 479,729 + -0.2 479,729 + -0.7 479,730 - +0.4 479,730 - +1.5 479,808 + rraB MCAODC_02335 0.18 +2.2 479,808 + rraB MCAODC_02335 0.18 -1.6 479,809 - rraB MCAODC_02335 0.18 -0.5 479,809 - rraB MCAODC_02335 0.18 -1.1 479,809 - rraB MCAODC_02335 0.18 -1.2 479,809 - rraB MCAODC_02335 0.18 -0.8 480,055 - rraB MCAODC_02335 0.77 -0.7 480,162 + -0.6 480,162 + +0.7 480,162 + -0.7 480,163 - -0.6 480,163 - -0.7 480,163 - +0.1 480,182 + +1.0 480,183 - +0.3 480,309 + -0.8 480,309 + +0.0 480,310 - +0.9 480,310 - -0.7 480,310 - +0.2 480,310 - -0.9 480,310 - +1.3 480,310 - -0.1 480,310 - -0.3 480,408 + -0.0 480,444 + argF MCAODC_02340 0.13 -1.8 480,445 - argF MCAODC_02340 0.13 -0.4 480,511 + argF MCAODC_02340 0.20 +3.3 480,511 + argF MCAODC_02340 0.20 -1.0 480,512 - argF MCAODC_02340 0.20 -0.6 480,531 + argF MCAODC_02340 0.22 -0.3 480,535 + argF MCAODC_02340 0.22 -1.5 480,535 + argF MCAODC_02340 0.22 -1.0 480,535 + argF MCAODC_02340 0.22 -0.2 480,536 - argF MCAODC_02340 0.22 -2.0 480,536 - argF MCAODC_02340 0.22 -1.1 480,536 - argF MCAODC_02340 0.22 +0.8 480,567 + argF MCAODC_02340 0.26 -1.5 480,567 + argF MCAODC_02340 0.26 -0.9 480,610 + argF MCAODC_02340 0.30 -1.0 480,610 + argF MCAODC_02340 0.30 -1.6 480,610 + argF MCAODC_02340 0.30 -0.3 480,610 + argF MCAODC_02340 0.30 +0.7 480,611 - argF MCAODC_02340 0.30 -0.6 480,611 - argF MCAODC_02340 0.30 -0.8 480,611 - argF MCAODC_02340 0.30 +0.0 480,667 + argF MCAODC_02340 0.36 +0.1 480,668 - argF MCAODC_02340 0.36 -0.8 480,668 - argF MCAODC_02340 0.36 +0.1 480,791 - argF MCAODC_02340 0.48 -1.8 480,791 - argF MCAODC_02340 0.48 -1.9 480,791 - argF MCAODC_02340 0.48 +0.2 480,808 - argF MCAODC_02340 0.50 +0.7 480,808 - argF MCAODC_02340 0.50 -1.2 480,808 - argF MCAODC_02340 0.50 +0.2 480,948 + argF MCAODC_02340 0.63 -0.1 480,965 + argF MCAODC_02340 0.65 -1.6 480,966 - argF MCAODC_02340 0.65 -2.3 480,966 - argF MCAODC_02340 0.65 -0.5 480,966 - argF MCAODC_02340 0.65 -1.1 480,993 + argF MCAODC_02340 0.68 -3.5 480,993 + argF MCAODC_02340 0.68 -1.0 480,993 + argF MCAODC_02340 0.68 +0.7 480,994 - argF MCAODC_02340 0.68 -0.6 480,994 - argF MCAODC_02340 0.68 -0.1 480,998 - argF MCAODC_02340 0.68 +0.5 480,998 - argF MCAODC_02340 0.68 -0.7 481,000 - argF MCAODC_02340 0.69 +0.1 481,007 + argF MCAODC_02340 0.69 -0.9 481,007 + argF MCAODC_02340 0.69 +0.1 481,007 + argF MCAODC_02340 0.69 -2.1 481,007 + argF MCAODC_02340 0.69 -0.1 481,008 - argF MCAODC_02340 0.69 +0.6 481,008 - argF MCAODC_02340 0.69 -2.0 481,008 - argF MCAODC_02340 0.69 -1.2 481,059 - argF MCAODC_02340 0.75 -1.1 481,059 - argF MCAODC_02340 0.75 -2.4
Or see this region's nucleotide sequence