Experiment: Survival; Treatment=20250415-U1-SZ1-UF; TreatmentDuration=24hrs; Outgrowth=R2A_Kan10_Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABCV34_RS15710 and ABCV34_RS15715 are separated by 3 nucleotides ABCV34_RS15715 and ABCV34_RS15720 are separated by 5 nucleotides ABCV34_RS15720 and ABCV34_RS15725 overlap by 8 nucleotides ABCV34_RS15725 and ABCV34_RS15730 are separated by 184 nucleotides ABCV34_RS15730 and ABCV34_RS15735 are separated by 138 nucleotides
ABCV34_RS15710: ABCV34_RS15710 - YfjI family protein, at 3,326,262 to 3,327,818
_RS15710
ABCV34_RS15715: ABCV34_RS15715 - hypothetical protein, at 3,327,822 to 3,328,361
_RS15715
ABCV34_RS15720: ABCV34_RS15720 - single-stranded DNA-binding protein, at 3,328,367 to 3,328,702
_RS15720
ABCV34_RS15725: ABCV34_RS15725 - hypothetical protein, at 3,328,695 to 3,328,985
_RS15725
ABCV34_RS15730: ABCV34_RS15730 - hypothetical protein, at 3,329,170 to 3,329,778
_RS15730
ABCV34_RS15735: ABCV34_RS15735 - hypothetical protein, at 3,329,917 to 3,330,342
_RS15735
Position (kb)
3328
3329 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3327.726 kb on + strand at 3327.727 kb on - strand at 3327.727 kb on - strand at 3327.727 kb on - strand at 3327.789 kb on + strand at 3327.789 kb on + strand at 3327.789 kb on + strand at 3327.789 kb on + strand at 3327.789 kb on + strand at 3327.789 kb on + strand at 3327.789 kb on + strand at 3327.789 kb on + strand at 3327.790 kb on - strand at 3327.816 kb on + strand at 3327.817 kb on - strand at 3327.831 kb on + strand at 3327.831 kb on + strand at 3327.831 kb on + strand at 3327.832 kb on - strand at 3327.832 kb on - strand at 3327.849 kb on - strand at 3327.868 kb on - strand at 3327.868 kb on - strand at 3327.874 kb on + strand at 3327.875 kb on - strand at 3327.932 kb on + strand, within ABCV34_RS15715 at 3327.932 kb on + strand, within ABCV34_RS15715 at 3327.933 kb on - strand, within ABCV34_RS15715 at 3327.933 kb on - strand, within ABCV34_RS15715 at 3327.933 kb on - strand, within ABCV34_RS15715 at 3327.933 kb on - strand, within ABCV34_RS15715 at 3327.933 kb on - strand, within ABCV34_RS15715 at 3327.933 kb on - strand, within ABCV34_RS15715 at 3327.933 kb on - strand, within ABCV34_RS15715 at 3327.968 kb on + strand, within ABCV34_RS15715 at 3327.968 kb on + strand, within ABCV34_RS15715 at 3327.969 kb on - strand, within ABCV34_RS15715 at 3328.031 kb on + strand, within ABCV34_RS15715 at 3328.031 kb on + strand, within ABCV34_RS15715 at 3328.032 kb on - strand, within ABCV34_RS15715 at 3328.032 kb on - strand, within ABCV34_RS15715 at 3328.032 kb on - strand, within ABCV34_RS15715 at 3328.144 kb on + strand, within ABCV34_RS15715 at 3328.144 kb on + strand, within ABCV34_RS15715 at 3328.144 kb on + strand, within ABCV34_RS15715 at 3328.145 kb on - strand, within ABCV34_RS15715 at 3328.145 kb on - strand, within ABCV34_RS15715 at 3328.175 kb on + strand, within ABCV34_RS15715 at 3328.175 kb on + strand, within ABCV34_RS15715 at 3328.175 kb on + strand, within ABCV34_RS15715 at 3328.176 kb on - strand, within ABCV34_RS15715 at 3328.192 kb on + strand, within ABCV34_RS15715 at 3328.193 kb on - strand, within ABCV34_RS15715 at 3328.193 kb on - strand, within ABCV34_RS15715 at 3328.193 kb on - strand, within ABCV34_RS15715 at 3328.230 kb on + strand, within ABCV34_RS15715 at 3328.231 kb on - strand, within ABCV34_RS15715 at 3328.231 kb on - strand, within ABCV34_RS15715 at 3328.231 kb on - strand, within ABCV34_RS15715 at 3328.231 kb on - strand, within ABCV34_RS15715 at 3328.231 kb on - strand, within ABCV34_RS15715 at 3328.234 kb on - strand, within ABCV34_RS15715 at 3328.383 kb on + strand at 3328.383 kb on + strand at 3328.383 kb on + strand at 3328.384 kb on - strand at 3328.398 kb on - strand at 3328.398 kb on - strand at 3328.398 kb on - strand at 3328.429 kb on + strand, within ABCV34_RS15720 at 3328.429 kb on + strand, within ABCV34_RS15720 at 3328.429 kb on + strand, within ABCV34_RS15720 at 3328.429 kb on + strand, within ABCV34_RS15720 at 3328.429 kb on + strand, within ABCV34_RS15720 at 3328.429 kb on + strand, within ABCV34_RS15720 at 3328.430 kb on - strand, within ABCV34_RS15720 at 3328.430 kb on - strand, within ABCV34_RS15720 at 3328.430 kb on - strand, within ABCV34_RS15720 at 3328.430 kb on - strand, within ABCV34_RS15720 at 3328.430 kb on - strand, within ABCV34_RS15720 at 3328.430 kb on - strand, within ABCV34_RS15720 at 3328.439 kb on + strand, within ABCV34_RS15720 at 3328.439 kb on + strand, within ABCV34_RS15720 at 3328.439 kb on + strand, within ABCV34_RS15720 at 3328.439 kb on + strand, within ABCV34_RS15720 at 3328.440 kb on - strand, within ABCV34_RS15720 at 3328.440 kb on - strand, within ABCV34_RS15720 at 3328.440 kb on - strand, within ABCV34_RS15720 at 3328.441 kb on + strand, within ABCV34_RS15720 at 3328.441 kb on + strand, within ABCV34_RS15720 at 3328.441 kb on + strand, within ABCV34_RS15720 at 3328.442 kb on - strand, within ABCV34_RS15720 at 3328.442 kb on - strand, within ABCV34_RS15720 at 3328.442 kb on - strand, within ABCV34_RS15720 at 3328.442 kb on - strand, within ABCV34_RS15720 at 3328.589 kb on + strand, within ABCV34_RS15720 at 3328.590 kb on - strand, within ABCV34_RS15720 at 3328.606 kb on + strand, within ABCV34_RS15720 at 3328.606 kb on + strand, within ABCV34_RS15720 at 3328.606 kb on + strand, within ABCV34_RS15720 at 3328.606 kb on + strand, within ABCV34_RS15720 at 3328.606 kb on + strand, within ABCV34_RS15720 at 3328.606 kb on + strand, within ABCV34_RS15720 at 3328.606 kb on + strand, within ABCV34_RS15720 at 3328.606 kb on + strand, within ABCV34_RS15720 at 3328.606 kb on + strand, within ABCV34_RS15720 at 3328.607 kb on - strand, within ABCV34_RS15720 at 3328.607 kb on - strand, within ABCV34_RS15720 at 3328.607 kb on - strand, within ABCV34_RS15720 at 3328.607 kb on - strand, within ABCV34_RS15720 at 3328.607 kb on - strand, within ABCV34_RS15720 at 3328.607 kb on - strand, within ABCV34_RS15720 at 3328.607 kb on - strand, within ABCV34_RS15720 at 3328.607 kb on - strand, within ABCV34_RS15720 at 3328.607 kb on - strand, within ABCV34_RS15720 at 3328.607 kb on - strand, within ABCV34_RS15720 at 3328.607 kb on - strand, within ABCV34_RS15720 at 3328.607 kb on - strand, within ABCV34_RS15720 at 3328.607 kb on - strand, within ABCV34_RS15720 at 3328.607 kb on - strand, within ABCV34_RS15720 at 3328.629 kb on + strand, within ABCV34_RS15720 at 3328.629 kb on + strand, within ABCV34_RS15720 at 3328.629 kb on + strand, within ABCV34_RS15720 at 3328.630 kb on - strand, within ABCV34_RS15720 at 3328.630 kb on - strand, within ABCV34_RS15720 at 3328.630 kb on - strand, within ABCV34_RS15720 at 3328.630 kb on - strand, within ABCV34_RS15720 at 3328.630 kb on - strand, within ABCV34_RS15720 at 3328.854 kb on + strand, within ABCV34_RS15725 at 3328.855 kb on - strand, within ABCV34_RS15725 at 3328.855 kb on - strand, within ABCV34_RS15725 at 3328.927 kb on + strand, within ABCV34_RS15725 at 3328.928 kb on - strand, within ABCV34_RS15725 at 3328.972 kb on + strand at 3328.973 kb on - strand at 3328.973 kb on - strand at 3328.996 kb on + strand at 3328.996 kb on + strand at 3328.996 kb on + strand at 3328.996 kb on + strand at 3328.997 kb on - strand at 3328.997 kb on - strand at 3328.997 kb on - strand at 3328.997 kb on - strand at 3328.997 kb on - strand at 3328.997 kb on - strand at 3329.087 kb on + strand at 3329.087 kb on + strand at 3329.128 kb on - strand at 3329.195 kb on - strand at 3329.195 kb on - strand at 3329.319 kb on + strand, within ABCV34_RS15730 at 3329.319 kb on + strand, within ABCV34_RS15730 at 3329.471 kb on + strand, within ABCV34_RS15730 at 3329.471 kb on + strand, within ABCV34_RS15730 at 3329.472 kb on - strand, within ABCV34_RS15730 at 3329.472 kb on - strand, within ABCV34_RS15730 at 3329.518 kb on + strand, within ABCV34_RS15730 at 3329.518 kb on + strand, within ABCV34_RS15730 at 3329.518 kb on + strand, within ABCV34_RS15730 at 3329.518 kb on + strand, within ABCV34_RS15730 at 3329.518 kb on + strand, within ABCV34_RS15730 at 3329.518 kb on + strand, within ABCV34_RS15730 at 3329.519 kb on - strand, within ABCV34_RS15730 at 3329.519 kb on - strand, within ABCV34_RS15730 at 3329.519 kb on - strand, within ABCV34_RS15730 at 3329.545 kb on + strand, within ABCV34_RS15730 at 3329.545 kb on + strand, within ABCV34_RS15730 at 3329.546 kb on - strand, within ABCV34_RS15730 at 3329.546 kb on - strand, within ABCV34_RS15730 at 3329.569 kb on - strand, within ABCV34_RS15730 at 3329.569 kb on - strand, within ABCV34_RS15730 at 3329.708 kb on + strand, within ABCV34_RS15730 at 3329.708 kb on + strand, within ABCV34_RS15730 at 3329.709 kb on - strand, within ABCV34_RS15730 at 3329.709 kb on - strand, within ABCV34_RS15730 at 3329.709 kb on - strand, within ABCV34_RS15730 at 3329.709 kb on - strand, within ABCV34_RS15730 at 3329.719 kb on + strand at 3329.719 kb on + strand at 3329.720 kb on - strand at 3329.745 kb on + strand at 3329.745 kb on + strand at 3329.746 kb on - strand at 3329.746 kb on - strand at 3329.746 kb on - strand at 3329.746 kb on - strand at 3329.776 kb on + strand at 3329.776 kb on + strand at 3329.776 kb on + strand at 3329.777 kb on - strand at 3329.777 kb on - strand at 3329.777 kb on - strand at 3329.815 kb on + strand at 3329.878 kb on + strand at 3329.878 kb on + strand at 3329.878 kb on + strand at 3329.878 kb on + strand at 3329.878 kb on + strand at 3329.878 kb on + strand at 3329.879 kb on - strand at 3329.879 kb on - strand at 3329.880 kb on + strand at 3329.880 kb on + strand at 3329.880 kb on + strand at 3329.880 kb on + strand at 3329.880 kb on + strand at 3329.880 kb on + strand at 3329.880 kb on + strand at 3329.880 kb on + strand at 3329.880 kb on + strand at 3329.880 kb on + strand at 3329.881 kb on - strand at 3329.881 kb on - strand at 3329.881 kb on - strand at 3329.881 kb on - strand at 3329.881 kb on - strand at 3329.881 kb on - strand at 3329.884 kb on + strand at 3329.884 kb on + strand at 3329.884 kb on + strand at 3329.884 kb on + strand at 3329.884 kb on + strand at 3329.884 kb on + strand at 3329.884 kb on + strand at 3329.884 kb on + strand at 3329.885 kb on - strand at 3329.885 kb on - strand at 3329.885 kb on - strand at 3329.885 kb on - strand at 3329.885 kb on - strand at 3329.885 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=20250415-U1-SZ1-UF; TreatmentDuration=24hrs; Outgrowth=R2A_Kan10_Lactate remove 3,327,726 + +0.8 3,327,727 - -3.4 3,327,727 - -3.4 3,327,727 - -1.7 3,327,789 + -1.4 3,327,789 + -1.8 3,327,789 + -1.4 3,327,789 + -1.6 3,327,789 + -1.2 3,327,789 + -3.4 3,327,789 + -0.6 3,327,789 + -2.6 3,327,790 - -2.2 3,327,816 + -1.4 3,327,817 - -1.3 3,327,831 + -1.4 3,327,831 + -1.9 3,327,831 + -2.8 3,327,832 - -1.1 3,327,832 - -2.5 3,327,849 - -1.7 3,327,868 - -0.2 3,327,868 - -4.6 3,327,874 + -1.5 3,327,875 - -2.7 3,327,932 + ABCV34_RS15715 0.20 -1.3 3,327,932 + ABCV34_RS15715 0.20 +3.1 3,327,933 - ABCV34_RS15715 0.21 -1.3 3,327,933 - ABCV34_RS15715 0.21 -0.5 3,327,933 - ABCV34_RS15715 0.21 -0.8 3,327,933 - ABCV34_RS15715 0.21 -4.4 3,327,933 - ABCV34_RS15715 0.21 +0.3 3,327,933 - ABCV34_RS15715 0.21 -1.1 3,327,933 - ABCV34_RS15715 0.21 -2.0 3,327,968 + ABCV34_RS15715 0.27 -1.4 3,327,968 + ABCV34_RS15715 0.27 -3.5 3,327,969 - ABCV34_RS15715 0.27 -3.6 3,328,031 + ABCV34_RS15715 0.39 -1.9 3,328,031 + ABCV34_RS15715 0.39 -0.3 3,328,032 - ABCV34_RS15715 0.39 +1.3 3,328,032 - ABCV34_RS15715 0.39 -0.9 3,328,032 - ABCV34_RS15715 0.39 -4.6 3,328,144 + ABCV34_RS15715 0.60 -3.1 3,328,144 + ABCV34_RS15715 0.60 -0.4 3,328,144 + ABCV34_RS15715 0.60 -2.7 3,328,145 - ABCV34_RS15715 0.60 -2.3 3,328,145 - ABCV34_RS15715 0.60 +0.1 3,328,175 + ABCV34_RS15715 0.65 -2.4 3,328,175 + ABCV34_RS15715 0.65 -1.5 3,328,175 + ABCV34_RS15715 0.65 -4.0 3,328,176 - ABCV34_RS15715 0.66 -1.0 3,328,192 + ABCV34_RS15715 0.69 -4.1 3,328,193 - ABCV34_RS15715 0.69 -2.2 3,328,193 - ABCV34_RS15715 0.69 -3.3 3,328,193 - ABCV34_RS15715 0.69 -2.6 3,328,230 + ABCV34_RS15715 0.76 -0.7 3,328,231 - ABCV34_RS15715 0.76 -0.4 3,328,231 - ABCV34_RS15715 0.76 -1.7 3,328,231 - ABCV34_RS15715 0.76 +0.7 3,328,231 - ABCV34_RS15715 0.76 -1.5 3,328,231 - ABCV34_RS15715 0.76 -3.2 3,328,234 - ABCV34_RS15715 0.76 -2.0 3,328,383 + -1.4 3,328,383 + +0.5 3,328,383 + -1.3 3,328,384 - -3.2 3,328,398 - -1.4 3,328,398 - -3.3 3,328,398 - -3.4 3,328,429 + ABCV34_RS15720 0.18 -3.7 3,328,429 + ABCV34_RS15720 0.18 -1.4 3,328,429 + ABCV34_RS15720 0.18 -0.1 3,328,429 + ABCV34_RS15720 0.18 -2.2 3,328,429 + ABCV34_RS15720 0.18 +0.5 3,328,429 + ABCV34_RS15720 0.18 +0.3 3,328,430 - ABCV34_RS15720 0.19 +0.2 3,328,430 - ABCV34_RS15720 0.19 -1.1 3,328,430 - ABCV34_RS15720 0.19 +0.0 3,328,430 - ABCV34_RS15720 0.19 -3.1 3,328,430 - ABCV34_RS15720 0.19 -1.3 3,328,430 - ABCV34_RS15720 0.19 +2.5 3,328,439 + ABCV34_RS15720 0.21 -2.0 3,328,439 + ABCV34_RS15720 0.21 -2.7 3,328,439 + ABCV34_RS15720 0.21 -1.2 3,328,439 + ABCV34_RS15720 0.21 +1.4 3,328,440 - ABCV34_RS15720 0.22 -1.3 3,328,440 - ABCV34_RS15720 0.22 -0.5 3,328,440 - ABCV34_RS15720 0.22 -2.3 3,328,441 + ABCV34_RS15720 0.22 -3.5 3,328,441 + ABCV34_RS15720 0.22 -2.7 3,328,441 + ABCV34_RS15720 0.22 -1.1 3,328,442 - ABCV34_RS15720 0.22 -3.9 3,328,442 - ABCV34_RS15720 0.22 -4.5 3,328,442 - ABCV34_RS15720 0.22 -2.0 3,328,442 - ABCV34_RS15720 0.22 -4.6 3,328,589 + ABCV34_RS15720 0.66 -1.8 3,328,590 - ABCV34_RS15720 0.66 -2.5 3,328,606 + ABCV34_RS15720 0.71 +1.3 3,328,606 + ABCV34_RS15720 0.71 -3.6 3,328,606 + ABCV34_RS15720 0.71 +1.4 3,328,606 + ABCV34_RS15720 0.71 -2.0 3,328,606 + ABCV34_RS15720 0.71 -2.8 3,328,606 + ABCV34_RS15720 0.71 -3.1 3,328,606 + ABCV34_RS15720 0.71 -4.2 3,328,606 + ABCV34_RS15720 0.71 +0.7 3,328,606 + ABCV34_RS15720 0.71 -0.5 3,328,607 - ABCV34_RS15720 0.71 +1.4 3,328,607 - ABCV34_RS15720 0.71 -1.7 3,328,607 - ABCV34_RS15720 0.71 +1.7 3,328,607 - ABCV34_RS15720 0.71 -0.6 3,328,607 - ABCV34_RS15720 0.71 +1.3 3,328,607 - ABCV34_RS15720 0.71 -1.2 3,328,607 - ABCV34_RS15720 0.71 -4.5 3,328,607 - ABCV34_RS15720 0.71 -0.9 3,328,607 - ABCV34_RS15720 0.71 -3.3 3,328,607 - ABCV34_RS15720 0.71 -0.7 3,328,607 - ABCV34_RS15720 0.71 -2.8 3,328,607 - ABCV34_RS15720 0.71 -3.3 3,328,607 - ABCV34_RS15720 0.71 -2.3 3,328,607 - ABCV34_RS15720 0.71 -3.7 3,328,629 + ABCV34_RS15720 0.78 -2.3 3,328,629 + ABCV34_RS15720 0.78 -1.8 3,328,629 + ABCV34_RS15720 0.78 -1.4 3,328,630 - ABCV34_RS15720 0.78 +0.2 3,328,630 - ABCV34_RS15720 0.78 -0.1 3,328,630 - ABCV34_RS15720 0.78 -0.4 3,328,630 - ABCV34_RS15720 0.78 -2.3 3,328,630 - ABCV34_RS15720 0.78 -3.0 3,328,854 + ABCV34_RS15725 0.55 -3.0 3,328,855 - ABCV34_RS15725 0.55 -1.7 3,328,855 - ABCV34_RS15725 0.55 -0.6 3,328,927 + ABCV34_RS15725 0.80 +2.8 3,328,928 - ABCV34_RS15725 0.80 -2.5 3,328,972 + -1.3 3,328,973 - -0.0 3,328,973 - -2.0 3,328,996 + -1.2 3,328,996 + -2.8 3,328,996 + +0.7 3,328,996 + -2.7 3,328,997 - -4.6 3,328,997 - -0.6 3,328,997 - +1.3 3,328,997 - -1.7 3,328,997 - +0.4 3,328,997 - -3.5 3,329,087 + -2.0 3,329,087 + -0.7 3,329,128 - -2.5 3,329,195 - -2.2 3,329,195 - +0.0 3,329,319 + ABCV34_RS15730 0.24 -2.5 3,329,319 + ABCV34_RS15730 0.24 +1.8 3,329,471 + ABCV34_RS15730 0.49 -2.1 3,329,471 + ABCV34_RS15730 0.49 +0.1 3,329,472 - ABCV34_RS15730 0.50 -2.2 3,329,472 - ABCV34_RS15730 0.50 -1.1 3,329,518 + ABCV34_RS15730 0.57 -1.7 3,329,518 + ABCV34_RS15730 0.57 +0.6 3,329,518 + ABCV34_RS15730 0.57 -0.1 3,329,518 + ABCV34_RS15730 0.57 -3.0 3,329,518 + ABCV34_RS15730 0.57 -2.1 3,329,518 + ABCV34_RS15730 0.57 -1.9 3,329,519 - ABCV34_RS15730 0.57 -1.4 3,329,519 - ABCV34_RS15730 0.57 -0.9 3,329,519 - ABCV34_RS15730 0.57 -1.7 3,329,545 + ABCV34_RS15730 0.62 -1.1 3,329,545 + ABCV34_RS15730 0.62 +0.5 3,329,546 - ABCV34_RS15730 0.62 -0.3 3,329,546 - ABCV34_RS15730 0.62 -2.7 3,329,569 - ABCV34_RS15730 0.66 -3.1 3,329,569 - ABCV34_RS15730 0.66 -2.3 3,329,708 + ABCV34_RS15730 0.88 -4.4 3,329,708 + ABCV34_RS15730 0.88 +0.9 3,329,709 - ABCV34_RS15730 0.89 +2.7 3,329,709 - ABCV34_RS15730 0.89 -1.5 3,329,709 - ABCV34_RS15730 0.89 -1.1 3,329,709 - ABCV34_RS15730 0.89 +0.9 3,329,719 + -2.4 3,329,719 + -1.5 3,329,720 - -2.6 3,329,745 + -2.3 3,329,745 + -1.1 3,329,746 - +0.8 3,329,746 - -2.7 3,329,746 - +0.5 3,329,746 - -1.0 3,329,776 + -2.3 3,329,776 + -1.7 3,329,776 + -0.3 3,329,777 - -2.5 3,329,777 - +2.3 3,329,777 - -0.8 3,329,815 + -4.7 3,329,878 + -0.2 3,329,878 + -3.2 3,329,878 + -2.4 3,329,878 + -2.8 3,329,878 + -2.8 3,329,878 + -0.7 3,329,879 - -1.3 3,329,879 - +0.3 3,329,880 + -2.0 3,329,880 + -3.4 3,329,880 + -1.8 3,329,880 + -1.6 3,329,880 + -1.3 3,329,880 + +0.9 3,329,880 + -2.9 3,329,880 + +0.3 3,329,880 + -0.9 3,329,880 + -1.5 3,329,881 - +0.2 3,329,881 - -0.1 3,329,881 - -1.4 3,329,881 - -4.1 3,329,881 - -2.0 3,329,881 - -2.3 3,329,884 + -1.3 3,329,884 + -0.8 3,329,884 + +0.1 3,329,884 + -1.5 3,329,884 + -1.3 3,329,884 + -0.9 3,329,884 + -0.9 3,329,884 + +0.1 3,329,885 - +1.9 3,329,885 - -1.4 3,329,885 - -2.9 3,329,885 - -0.1 3,329,885 - -1.5 3,329,885 - +1.0
Or see this region's nucleotide sequence