Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ile2_trna and Lys_trna are separated by 1 nucleotides Lys_trna and Thr_trna are separated by 2 nucleotides Thr_trna and Gly_trna are separated by 3 nucleotides Gly_trna and HEPCGN_03750 are separated by 292 nucleotides HEPCGN_03750 and HEPCGN_03755 are separated by 4 nucleotides HEPCGN_03755 and HEPCGN_03760 are separated by 63 nucleotides HEPCGN_03760 and HEPCGN_03765 overlap by 4 nucleotides
HEPCGN_03730: Ile2_trna - tRNA-Ile2(cat), at 661,305 to 661,380
_trna
HEPCGN_03735: Lys_trna - tRNA-Lys(ttt), at 661,382 to 661,458
_trna
HEPCGN_03740: Thr_trna - tRNA-Thr(tgt), at 661,461 to 661,536
_trna
HEPCGN_03745: Gly_trna - tRNA-Gly(tcc), at 661,540 to 661,614
_trna
HEPCGN_03750: HEPCGN_03750 - holin, at 661,907 to 662,122
_03750
HEPCGN_03755: HEPCGN_03755 - DUF1327 domain-containing protein, at 662,127 to 662,477
_03755
HEPCGN_03760: HEPCGN_03760 - lysozyme, at 662,541 to 663,074
_03760
HEPCGN_03765: HEPCGN_03765 - lysis protein, at 663,071 to 663,538
_03765
Position (kb) 662
663 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 661.193 kb on + strand at 661.203 kb on - strand at 661.232 kb on + strand at 661.537 kb on + strand at 661.563 kb on - strand, within Gly_trna at 661.563 kb on - strand, within Gly_trna at 661.671 kb on - strand at 661.671 kb on - strand at 661.676 kb on - strand at 661.744 kb on + strand at 661.809 kb on + strand at 661.809 kb on + strand at 661.810 kb on - strand at 661.810 kb on - strand at 661.811 kb on + strand at 661.811 kb on + strand at 661.812 kb on - strand at 661.813 kb on + strand at 662.060 kb on + strand, within HEPCGN_03750 at 662.060 kb on + strand, within HEPCGN_03750 at 662.412 kb on + strand, within HEPCGN_03755 at 662.476 kb on - strand at 662.476 kb on - strand at 662.496 kb on + strand at 662.888 kb on + strand, within HEPCGN_03760 at 662.910 kb on + strand, within HEPCGN_03760 at 662.995 kb on + strand, within HEPCGN_03760 at 662.996 kb on - strand, within HEPCGN_03760 at 663.091 kb on + strand at 663.096 kb on - strand at 663.168 kb on - strand, within HEPCGN_03765
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 661,193 + -0.2 661,203 - +0.3 661,232 + +0.6 661,537 + -0.1 661,563 - Gly_trna HEPCGN_03745 0.31 +0.6 661,563 - Gly_trna HEPCGN_03745 0.31 +0.1 661,671 - -1.0 661,671 - +0.3 661,676 - -2.8 661,744 + +0.2 661,809 + -0.5 661,809 + -0.5 661,810 - -0.2 661,810 - -0.2 661,811 + -0.6 661,811 + +2.5 661,812 - -1.2 661,813 + +0.2 662,060 + HEPCGN_03750 0.71 -0.7 662,060 + HEPCGN_03750 0.71 +1.0 662,412 + HEPCGN_03755 0.81 -1.1 662,476 - -1.7 662,476 - -0.0 662,496 + -1.8 662,888 + HEPCGN_03760 0.65 +0.1 662,910 + HEPCGN_03760 0.69 +0.1 662,995 + HEPCGN_03760 0.85 +0.6 662,996 - HEPCGN_03760 0.85 -0.5 663,091 + -1.7 663,096 - -0.7 663,168 - HEPCGN_03765 0.21 -0.7
Or see this region's nucleotide sequence