Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt miaB and ubiF are separated by 145 nucleotides ubiF and Gln_trna are separated by 215 nucleotides Gln_trna and Met_trna are separated by 48 nucleotides Met_trna and Gln_trna are separated by 15 nucleotides Gln_trna and Gln_trna are separated by 34 nucleotides Gln_trna and Leu_trna are separated by 23 nucleotides Leu_trna and Met_trna are separated by 8 nucleotides
HEPCGN_01005: miaB - tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB, at 128,003 to 129,427
miaB
HEPCGN_01010: ubiF - 3-demethoxyubiquinol 3-hydroxylase, at 129,573 to 130,748
ubiF
HEPCGN_01015: Gln_trna - tRNA-Gln(ctg), at 130,964 to 131,038
_trna
HEPCGN_01020: Met_trna - tRNA-Met(cat), at 131,087 to 131,163
_trna
HEPCGN_01025: Gln_trna - tRNA-Gln(ttg), at 131,179 to 131,253
_trna
HEPCGN_01030: Gln_trna - tRNA-Gln(ttg), at 131,288 to 131,362
_trna
HEPCGN_01035: Leu_trna - tRNA-Leu(tag), at 131,386 to 131,470
_trna
HEPCGN_01040: Met_trna - tRNA-Met(cat), at 131,479 to 131,555
_trna
Position (kb) 129
130
131 Strain fitness (log2 ratio) -1
0
1
2 at 128.584 kb on + strand, within miaB at 128.925 kb on + strand, within miaB at 128.926 kb on - strand, within miaB at 128.940 kb on + strand, within miaB at 128.941 kb on - strand, within miaB at 128.941 kb on - strand, within miaB at 128.949 kb on + strand, within miaB at 128.949 kb on + strand, within miaB at 128.949 kb on + strand, within miaB at 128.950 kb on - strand, within miaB at 128.950 kb on - strand, within miaB at 129.046 kb on + strand, within miaB at 129.047 kb on - strand, within miaB at 129.135 kb on + strand, within miaB at 129.136 kb on - strand, within miaB at 129.136 kb on - strand, within miaB at 129.222 kb on - strand, within miaB at 129.379 kb on - strand at 129.406 kb on + strand at 129.407 kb on - strand at 129.407 kb on - strand at 129.470 kb on + strand at 129.475 kb on + strand at 129.543 kb on + strand at 129.550 kb on + strand at 129.550 kb on + strand at 129.664 kb on + strand at 129.962 kb on - strand, within ubiF at 130.136 kb on + strand, within ubiF at 130.254 kb on + strand, within ubiF at 130.646 kb on + strand at 130.731 kb on + strand at 130.731 kb on + strand at 130.746 kb on + strand at 130.771 kb on - strand at 130.894 kb on - strand at 131.276 kb on - strand at 131.368 kb on - strand at 131.688 kb on - strand at 131.688 kb on - strand at 131.688 kb on - strand at 131.689 kb on + strand at 131.700 kb on + strand at 131.730 kb on + strand at 131.730 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 128,584 + miaB HEPCGN_01005 0.41 +0.1 128,925 + miaB HEPCGN_01005 0.65 -0.4 128,926 - miaB HEPCGN_01005 0.65 +1.2 128,940 + miaB HEPCGN_01005 0.66 +2.6 128,941 - miaB HEPCGN_01005 0.66 -0.4 128,941 - miaB HEPCGN_01005 0.66 +2.0 128,949 + miaB HEPCGN_01005 0.66 -0.5 128,949 + miaB HEPCGN_01005 0.66 +0.1 128,949 + miaB HEPCGN_01005 0.66 +0.0 128,950 - miaB HEPCGN_01005 0.66 +0.8 128,950 - miaB HEPCGN_01005 0.66 +0.5 129,046 + miaB HEPCGN_01005 0.73 -0.0 129,047 - miaB HEPCGN_01005 0.73 +0.1 129,135 + miaB HEPCGN_01005 0.79 +1.0 129,136 - miaB HEPCGN_01005 0.80 +0.2 129,136 - miaB HEPCGN_01005 0.80 -0.0 129,222 - miaB HEPCGN_01005 0.86 -0.9 129,379 - +0.3 129,406 + -0.8 129,407 - +0.5 129,407 - +1.3 129,470 + +0.1 129,475 + -1.1 129,543 + -0.1 129,550 + +0.0 129,550 + +1.6 129,664 + +0.5 129,962 - ubiF HEPCGN_01010 0.33 +0.8 130,136 + ubiF HEPCGN_01010 0.48 -0.5 130,254 + ubiF HEPCGN_01010 0.58 -0.8 130,646 + -0.8 130,731 + -1.6 130,731 + +0.6 130,746 + -0.3 130,771 - -0.6 130,894 - +0.3 131,276 - +0.9 131,368 - +0.6 131,688 - +0.8 131,688 - -0.9 131,688 - +0.6 131,689 + +1.0 131,700 + +0.3 131,730 + +0.2 131,730 + -0.3
Or see this region's nucleotide sequence