Experiment: Bas31
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisA and hisH overlap by 1 nucleotides hisH and hisB overlap by 1 nucleotides hisB and hisC overlap by 1 nucleotides
NOLOHH_15810: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 3,117,328 to 3,118,065
hisA
NOLOHH_15815: hisH - imidazole glycerol phosphate synthase subunit HisH, at 3,118,065 to 3,118,655
hisH
NOLOHH_15820: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 3,118,655 to 3,119,722
hisB
NOLOHH_15825: hisC - histidinol-phosphate transaminase, at 3,119,722 to 3,120,792
hisC
Position (kb) 3118
3119
3120 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 3117.695 kb on - strand, within hisA at 3117.871 kb on - strand, within hisA at 3117.871 kb on - strand, within hisA at 3117.962 kb on - strand, within hisA at 3117.962 kb on - strand, within hisA at 3117.962 kb on - strand, within hisA at 3118.139 kb on - strand, within hisH at 3118.184 kb on + strand, within hisH at 3118.184 kb on + strand, within hisH at 3118.185 kb on - strand, within hisH at 3118.185 kb on - strand, within hisH at 3118.185 kb on - strand, within hisH at 3118.237 kb on + strand, within hisH at 3118.766 kb on - strand, within hisB at 3118.880 kb on - strand, within hisB at 3118.880 kb on - strand, within hisB at 3118.880 kb on - strand, within hisB at 3118.907 kb on - strand, within hisB at 3119.050 kb on - strand, within hisB at 3119.141 kb on - strand, within hisB at 3119.346 kb on + strand, within hisB at 3119.346 kb on + strand, within hisB at 3119.347 kb on - strand, within hisB at 3119.741 kb on - strand at 3119.818 kb on - strand at 3119.831 kb on - strand, within hisC at 3119.860 kb on - strand, within hisC at 3119.898 kb on - strand, within hisC at 3119.961 kb on - strand, within hisC at 3120.166 kb on + strand, within hisC at 3120.233 kb on + strand, within hisC at 3120.234 kb on - strand, within hisC at 3120.234 kb on - strand, within hisC at 3120.341 kb on + strand, within hisC at 3120.341 kb on + strand, within hisC at 3120.342 kb on - strand, within hisC at 3120.382 kb on + strand, within hisC at 3120.575 kb on - strand, within hisC
Per-strain Table
Position Strand Gene LocusTag Fraction Bas31 remove 3,117,695 - hisA NOLOHH_15810 0.50 +2.9 3,117,871 - hisA NOLOHH_15810 0.74 -0.4 3,117,871 - hisA NOLOHH_15810 0.74 +1.2 3,117,962 - hisA NOLOHH_15810 0.86 +1.1 3,117,962 - hisA NOLOHH_15810 0.86 -0.7 3,117,962 - hisA NOLOHH_15810 0.86 +0.2 3,118,139 - hisH NOLOHH_15815 0.13 -1.1 3,118,184 + hisH NOLOHH_15815 0.20 -0.3 3,118,184 + hisH NOLOHH_15815 0.20 -0.1 3,118,185 - hisH NOLOHH_15815 0.20 +1.9 3,118,185 - hisH NOLOHH_15815 0.20 -0.4 3,118,185 - hisH NOLOHH_15815 0.20 -1.9 3,118,237 + hisH NOLOHH_15815 0.29 -0.8 3,118,766 - hisB NOLOHH_15820 0.10 -1.6 3,118,880 - hisB NOLOHH_15820 0.21 -0.7 3,118,880 - hisB NOLOHH_15820 0.21 +1.6 3,118,880 - hisB NOLOHH_15820 0.21 +0.1 3,118,907 - hisB NOLOHH_15820 0.24 +1.1 3,119,050 - hisB NOLOHH_15820 0.37 -0.3 3,119,141 - hisB NOLOHH_15820 0.46 -1.2 3,119,346 + hisB NOLOHH_15820 0.65 +2.3 3,119,346 + hisB NOLOHH_15820 0.65 +3.0 3,119,347 - hisB NOLOHH_15820 0.65 -0.4 3,119,741 - -0.3 3,119,818 - -1.6 3,119,831 - hisC NOLOHH_15825 0.10 +0.3 3,119,860 - hisC NOLOHH_15825 0.13 +1.7 3,119,898 - hisC NOLOHH_15825 0.16 -1.6 3,119,961 - hisC NOLOHH_15825 0.22 -0.6 3,120,166 + hisC NOLOHH_15825 0.41 +0.4 3,120,233 + hisC NOLOHH_15825 0.48 +0.1 3,120,234 - hisC NOLOHH_15825 0.48 +0.7 3,120,234 - hisC NOLOHH_15825 0.48 -1.4 3,120,341 + hisC NOLOHH_15825 0.58 -1.2 3,120,341 + hisC NOLOHH_15825 0.58 -0.9 3,120,342 - hisC NOLOHH_15825 0.58 -3.0 3,120,382 + hisC NOLOHH_15825 0.62 -0.4 3,120,575 - hisC NOLOHH_15825 0.80 -1.8
Or see this region's nucleotide sequence