Experiment: defined media with L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS08470 and LRK53_RS08475 overlap by 4 nucleotides LRK53_RS08475 and LRK53_RS08480 are separated by 142 nucleotides LRK53_RS08480 and LRK53_RS08485 are separated by 157 nucleotides
LRK53_RS08470: LRK53_RS08470 - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 1,903,095 to 1,904,162
_RS08470
LRK53_RS08475: LRK53_RS08475 - imidazole glycerol phosphate synthase subunit HisH, at 1,904,159 to 1,904,752
_RS08475
LRK53_RS08480: LRK53_RS08480 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 1,904,895 to 1,905,626
_RS08480
LRK53_RS08485: LRK53_RS08485 - imidazole glycerol phosphate synthase subunit HisF, at 1,905,784 to 1,906,560
_RS08485
Position (kb)
1904
1905
1906 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1903.926 kb on + strand, within LRK53_RS08470 at 1903.926 kb on + strand, within LRK53_RS08470 at 1903.926 kb on + strand, within LRK53_RS08470 at 1903.927 kb on - strand, within LRK53_RS08470 at 1903.927 kb on - strand, within LRK53_RS08470 at 1904.100 kb on - strand at 1904.100 kb on - strand at 1904.213 kb on + strand at 1904.214 kb on - strand at 1904.579 kb on + strand, within LRK53_RS08475 at 1904.579 kb on + strand, within LRK53_RS08475 at 1904.579 kb on + strand, within LRK53_RS08475 at 1904.579 kb on + strand, within LRK53_RS08475 at 1904.579 kb on + strand, within LRK53_RS08475 at 1904.580 kb on - strand, within LRK53_RS08475 at 1904.580 kb on - strand, within LRK53_RS08475 at 1904.580 kb on - strand, within LRK53_RS08475 at 1904.580 kb on - strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.595 kb on - strand, within LRK53_RS08475 at 1904.595 kb on - strand, within LRK53_RS08475 at 1904.595 kb on - strand, within LRK53_RS08475 at 1904.595 kb on - strand, within LRK53_RS08475 at 1904.595 kb on - strand, within LRK53_RS08475 at 1904.595 kb on - strand, within LRK53_RS08475 at 1904.595 kb on - strand, within LRK53_RS08475 at 1904.595 kb on - strand, within LRK53_RS08475 at 1904.595 kb on - strand, within LRK53_RS08475 at 1904.748 kb on + strand at 1904.748 kb on + strand at 1904.748 kb on + strand at 1904.748 kb on + strand at 1904.748 kb on + strand at 1904.748 kb on + strand at 1904.748 kb on + strand at 1904.748 kb on + strand at 1904.749 kb on - strand at 1904.749 kb on - strand at 1904.749 kb on - strand at 1904.749 kb on - strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.822 kb on + strand at 1904.822 kb on + strand at 1904.822 kb on + strand at 1904.822 kb on + strand at 1904.822 kb on + strand at 1904.822 kb on + strand at 1904.822 kb on + strand at 1904.823 kb on - strand at 1904.823 kb on - strand at 1904.823 kb on - strand at 1904.823 kb on - strand at 1904.823 kb on - strand at 1904.901 kb on + strand at 1904.901 kb on + strand at 1904.901 kb on + strand at 1904.901 kb on + strand at 1904.901 kb on + strand at 1904.901 kb on + strand at 1904.901 kb on + strand at 1904.902 kb on - strand at 1904.902 kb on - strand at 1904.902 kb on - strand at 1904.902 kb on - strand at 1904.902 kb on - strand at 1904.902 kb on - strand at 1904.902 kb on - strand at 1904.902 kb on - strand at 1904.902 kb on - strand at 1904.902 kb on - strand at 1904.911 kb on + strand at 1904.911 kb on + strand at 1904.911 kb on + strand at 1904.911 kb on + strand at 1904.911 kb on + strand at 1904.912 kb on - strand at 1904.967 kb on + strand at 1904.967 kb on + strand at 1904.985 kb on + strand, within LRK53_RS08480 at 1904.985 kb on + strand, within LRK53_RS08480 at 1904.985 kb on + strand, within LRK53_RS08480 at 1904.985 kb on + strand, within LRK53_RS08480 at 1904.985 kb on + strand, within LRK53_RS08480 at 1904.986 kb on - strand, within LRK53_RS08480 at 1904.986 kb on - strand, within LRK53_RS08480 at 1904.986 kb on - strand, within LRK53_RS08480 at 1905.019 kb on - strand, within LRK53_RS08480 at 1905.019 kb on - strand, within LRK53_RS08480 at 1905.148 kb on + strand, within LRK53_RS08480 at 1905.258 kb on + strand, within LRK53_RS08480 at 1905.258 kb on + strand, within LRK53_RS08480 at 1905.258 kb on + strand, within LRK53_RS08480 at 1905.259 kb on - strand, within LRK53_RS08480 at 1905.259 kb on - strand, within LRK53_RS08480 at 1905.378 kb on + strand, within LRK53_RS08480 at 1905.379 kb on - strand, within LRK53_RS08480 at 1905.622 kb on + strand at 1905.622 kb on + strand at 1905.622 kb on + strand at 1905.623 kb on - strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.846 kb on - strand at 1905.931 kb on + strand, within LRK53_RS08485 at 1905.931 kb on + strand, within LRK53_RS08485 at 1905.931 kb on + strand, within LRK53_RS08485 at 1905.932 kb on - strand, within LRK53_RS08485 at 1905.932 kb on - strand, within LRK53_RS08485 at 1905.932 kb on - strand, within LRK53_RS08485 at 1905.932 kb on - strand, within LRK53_RS08485 at 1906.213 kb on + strand, within LRK53_RS08485 at 1906.214 kb on - strand, within LRK53_RS08485 at 1906.214 kb on - strand, within LRK53_RS08485 at 1906.214 kb on - strand, within LRK53_RS08485 at 1906.214 kb on - strand, within LRK53_RS08485 at 1906.342 kb on + strand, within LRK53_RS08485 at 1906.502 kb on + strand at 1906.502 kb on + strand at 1906.502 kb on + strand at 1906.519 kb on + strand at 1906.519 kb on + strand at 1906.519 kb on + strand at 1906.519 kb on + strand at 1906.519 kb on + strand at 1906.520 kb on - strand at 1906.520 kb on - strand at 1906.520 kb on - strand at 1906.520 kb on - strand at 1906.520 kb on - strand at 1906.545 kb on - strand at 1906.551 kb on + strand at 1906.551 kb on + strand at 1906.551 kb on + strand at 1906.551 kb on + strand at 1906.552 kb on - strand at 1906.552 kb on - strand at 1906.552 kb on - strand at 1906.552 kb on - strand at 1906.552 kb on - strand at 1906.552 kb on - strand at 1906.558 kb on + strand at 1906.559 kb on - strand at 1906.559 kb on - strand at 1906.559 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with L-Serine remove 1,903,926 + LRK53_RS08470 0.78 +0.8 1,903,926 + LRK53_RS08470 0.78 -1.6 1,903,926 + LRK53_RS08470 0.78 -3.1 1,903,927 - LRK53_RS08470 0.78 -1.4 1,903,927 - LRK53_RS08470 0.78 -1.2 1,904,100 - -3.0 1,904,100 - -3.2 1,904,213 + -0.2 1,904,214 - -2.7 1,904,579 + LRK53_RS08475 0.71 -2.0 1,904,579 + LRK53_RS08475 0.71 -3.8 1,904,579 + LRK53_RS08475 0.71 -0.8 1,904,579 + LRK53_RS08475 0.71 -4.5 1,904,579 + LRK53_RS08475 0.71 -1.6 1,904,580 - LRK53_RS08475 0.71 -2.4 1,904,580 - LRK53_RS08475 0.71 -2.7 1,904,580 - LRK53_RS08475 0.71 -0.8 1,904,580 - LRK53_RS08475 0.71 -2.2 1,904,594 + LRK53_RS08475 0.73 -1.2 1,904,594 + LRK53_RS08475 0.73 -2.2 1,904,594 + LRK53_RS08475 0.73 -1.2 1,904,594 + LRK53_RS08475 0.73 -0.8 1,904,594 + LRK53_RS08475 0.73 -2.0 1,904,594 + LRK53_RS08475 0.73 -3.8 1,904,594 + LRK53_RS08475 0.73 +0.8 1,904,594 + LRK53_RS08475 0.73 -2.8 1,904,594 + LRK53_RS08475 0.73 -1.2 1,904,594 + LRK53_RS08475 0.73 -2.2 1,904,594 + LRK53_RS08475 0.73 -2.0 1,904,595 - LRK53_RS08475 0.73 -2.1 1,904,595 - LRK53_RS08475 0.73 -1.2 1,904,595 - LRK53_RS08475 0.73 -2.0 1,904,595 - LRK53_RS08475 0.73 -1.2 1,904,595 - LRK53_RS08475 0.73 -1.2 1,904,595 - LRK53_RS08475 0.73 -2.0 1,904,595 - LRK53_RS08475 0.73 -1.6 1,904,595 - LRK53_RS08475 0.73 -1.2 1,904,595 - LRK53_RS08475 0.73 -0.8 1,904,748 + -0.2 1,904,748 + -0.2 1,904,748 + -0.7 1,904,748 + +0.0 1,904,748 + -1.0 1,904,748 + -0.3 1,904,748 + -1.6 1,904,748 + -1.8 1,904,749 - -2.7 1,904,749 - -1.2 1,904,749 - -1.2 1,904,749 - -0.8 1,904,754 + -0.2 1,904,754 + -0.6 1,904,754 + -1.6 1,904,754 + -1.4 1,904,754 + -1.6 1,904,754 + -1.1 1,904,754 + -0.6 1,904,754 + -0.3 1,904,754 + -1.4 1,904,754 + -0.2 1,904,754 + -1.2 1,904,754 + +0.4 1,904,754 + -0.4 1,904,754 + -0.4 1,904,754 + -1.1 1,904,754 + -1.4 1,904,755 - +0.0 1,904,755 - -0.8 1,904,755 - -1.8 1,904,755 - -0.5 1,904,755 - -0.9 1,904,755 - -1.8 1,904,755 - -0.7 1,904,755 - -1.5 1,904,755 - -1.2 1,904,755 - -0.6 1,904,755 - -2.0 1,904,755 - -0.2 1,904,755 - +0.1 1,904,755 - -1.0 1,904,755 - +0.2 1,904,755 - -0.7 1,904,755 - -0.4 1,904,755 - +0.8 1,904,755 - +0.1 1,904,755 - -0.2 1,904,822 + -1.4 1,904,822 + +1.8 1,904,822 + +1.3 1,904,822 + +0.2 1,904,822 + +0.8 1,904,822 + -0.8 1,904,822 + -0.8 1,904,823 - -0.4 1,904,823 - -1.2 1,904,823 - -0.9 1,904,823 - -0.7 1,904,823 - +0.8 1,904,901 + -1.2 1,904,901 + -2.0 1,904,901 + -3.0 1,904,901 + -3.4 1,904,901 + -0.6 1,904,901 + -3.3 1,904,901 + -2.6 1,904,902 - -1.6 1,904,902 - -2.8 1,904,902 - -2.8 1,904,902 - -2.4 1,904,902 - +0.8 1,904,902 - -2.2 1,904,902 - -0.2 1,904,902 - -0.2 1,904,902 - -1.6 1,904,902 - -1.8 1,904,911 + -1.0 1,904,911 + -2.0 1,904,911 + +0.2 1,904,911 + -1.8 1,904,911 + +0.8 1,904,912 - -1.8 1,904,967 + -0.8 1,904,967 + -2.2 1,904,985 + LRK53_RS08480 0.12 -2.0 1,904,985 + LRK53_RS08480 0.12 -3.2 1,904,985 + LRK53_RS08480 0.12 -0.8 1,904,985 + LRK53_RS08480 0.12 -1.2 1,904,985 + LRK53_RS08480 0.12 -1.8 1,904,986 - LRK53_RS08480 0.12 -1.6 1,904,986 - LRK53_RS08480 0.12 -4.3 1,904,986 - LRK53_RS08480 0.12 -1.6 1,905,019 - LRK53_RS08480 0.17 -1.2 1,905,019 - LRK53_RS08480 0.17 -1.2 1,905,148 + LRK53_RS08480 0.35 -3.3 1,905,258 + LRK53_RS08480 0.50 -3.6 1,905,258 + LRK53_RS08480 0.50 -0.2 1,905,258 + LRK53_RS08480 0.50 -2.9 1,905,259 - LRK53_RS08480 0.50 -2.7 1,905,259 - LRK53_RS08480 0.50 +0.8 1,905,378 + LRK53_RS08480 0.66 -1.6 1,905,379 - LRK53_RS08480 0.66 -2.8 1,905,622 + -1.0 1,905,622 + +1.3 1,905,622 + -1.6 1,905,623 - -0.2 1,905,845 + -0.8 1,905,845 + -3.3 1,905,845 + -2.4 1,905,845 + -1.2 1,905,845 + -0.2 1,905,845 + -2.8 1,905,845 + -2.2 1,905,845 + -2.8 1,905,845 + -1.2 1,905,845 + -2.0 1,905,845 + -2.9 1,905,846 - -4.3 1,905,931 + LRK53_RS08485 0.19 -2.4 1,905,931 + LRK53_RS08485 0.19 -2.3 1,905,931 + LRK53_RS08485 0.19 -0.2 1,905,932 - LRK53_RS08485 0.19 -0.2 1,905,932 - LRK53_RS08485 0.19 -1.2 1,905,932 - LRK53_RS08485 0.19 -1.8 1,905,932 - LRK53_RS08485 0.19 -0.2 1,906,213 + LRK53_RS08485 0.55 -1.9 1,906,214 - LRK53_RS08485 0.55 -1.6 1,906,214 - LRK53_RS08485 0.55 -4.4 1,906,214 - LRK53_RS08485 0.55 -1.6 1,906,214 - LRK53_RS08485 0.55 -2.7 1,906,342 + LRK53_RS08485 0.72 -0.2 1,906,502 + -0.8 1,906,502 + -4.7 1,906,502 + -0.5 1,906,519 + -0.2 1,906,519 + -0.8 1,906,519 + -0.3 1,906,519 + -0.2 1,906,519 + -1.7 1,906,520 - -1.1 1,906,520 - -0.6 1,906,520 - -0.7 1,906,520 - -1.0 1,906,520 - -1.4 1,906,545 - -3.0 1,906,551 + +0.2 1,906,551 + +0.4 1,906,551 + +0.8 1,906,551 + -0.0 1,906,552 - -2.0 1,906,552 - -0.6 1,906,552 - -0.2 1,906,552 - -0.8 1,906,552 - -0.2 1,906,552 - -0.6 1,906,558 + +1.1 1,906,559 - -2.3 1,906,559 - +0.8 1,906,559 - -1.5
Or see this region's nucleotide sequence