Experiment: R2A with 3-octanone 10.6206 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS17285 and ABIE40_RS17290 are separated by 343 nucleotides ABIE40_RS17290 and ABIE40_RS17295 overlap by 4 nucleotides
ABIE40_RS17285: ABIE40_RS17285 - DUF4167 domain-containing protein, at 3,456,782 to 3,457,480
_RS17285
ABIE40_RS17290: ABIE40_RS17290 - peptide chain release factor N(5)-glutamine methyltransferase, at 3,457,824 to 3,458,696
_RS17290
ABIE40_RS17295: ABIE40_RS17295 - peptide chain release factor 1, at 3,458,693 to 3,459,772
_RS17295
Position (kb)
3457
3458
3459 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3457.150 kb on - strand, within ABIE40_RS17285 at 3457.517 kb on - strand at 3457.617 kb on - strand at 3457.617 kb on - strand at 3457.697 kb on - strand at 3457.748 kb on - strand at 3457.748 kb on - strand at 3457.787 kb on + strand at 3457.788 kb on - strand at 3458.008 kb on + strand, within ABIE40_RS17290 at 3458.009 kb on - strand, within ABIE40_RS17290 at 3458.009 kb on - strand, within ABIE40_RS17290 at 3458.102 kb on + strand, within ABIE40_RS17290 at 3458.103 kb on - strand, within ABIE40_RS17290 at 3458.103 kb on - strand, within ABIE40_RS17290 at 3458.104 kb on + strand, within ABIE40_RS17290 at 3458.104 kb on + strand, within ABIE40_RS17290 at 3458.104 kb on + strand, within ABIE40_RS17290 at 3458.105 kb on - strand, within ABIE40_RS17290 at 3458.105 kb on - strand, within ABIE40_RS17290 at 3458.105 kb on - strand, within ABIE40_RS17290 at 3458.105 kb on - strand, within ABIE40_RS17290 at 3458.144 kb on + strand, within ABIE40_RS17290 at 3458.145 kb on - strand, within ABIE40_RS17290 at 3458.166 kb on + strand, within ABIE40_RS17290 at 3458.166 kb on + strand, within ABIE40_RS17290 at 3458.166 kb on + strand, within ABIE40_RS17290 at 3458.167 kb on - strand, within ABIE40_RS17290 at 3458.167 kb on - strand, within ABIE40_RS17290 at 3458.238 kb on + strand, within ABIE40_RS17290 at 3458.238 kb on + strand, within ABIE40_RS17290 at 3458.238 kb on + strand, within ABIE40_RS17290 at 3458.238 kb on + strand, within ABIE40_RS17290 at 3458.293 kb on - strand, within ABIE40_RS17290 at 3458.358 kb on + strand, within ABIE40_RS17290 at 3458.358 kb on + strand, within ABIE40_RS17290 at 3458.359 kb on - strand, within ABIE40_RS17290 at 3458.376 kb on - strand, within ABIE40_RS17290 at 3458.544 kb on + strand, within ABIE40_RS17290 at 3458.584 kb on - strand, within ABIE40_RS17290 at 3458.584 kb on - strand, within ABIE40_RS17290
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 10.6206 mM remove 3,457,150 - ABIE40_RS17285 0.53 +1.7 3,457,517 - -2.8 3,457,617 - -0.5 3,457,617 - +0.2 3,457,697 - -0.1 3,457,748 - +0.3 3,457,748 - -0.9 3,457,787 + -0.8 3,457,788 - -1.1 3,458,008 + ABIE40_RS17290 0.21 +0.7 3,458,009 - ABIE40_RS17290 0.21 -0.4 3,458,009 - ABIE40_RS17290 0.21 -0.8 3,458,102 + ABIE40_RS17290 0.32 -2.6 3,458,103 - ABIE40_RS17290 0.32 -0.3 3,458,103 - ABIE40_RS17290 0.32 -2.5 3,458,104 + ABIE40_RS17290 0.32 -2.6 3,458,104 + ABIE40_RS17290 0.32 -0.7 3,458,104 + ABIE40_RS17290 0.32 +1.2 3,458,105 - ABIE40_RS17290 0.32 -2.3 3,458,105 - ABIE40_RS17290 0.32 -1.7 3,458,105 - ABIE40_RS17290 0.32 -1.4 3,458,105 - ABIE40_RS17290 0.32 -2.3 3,458,144 + ABIE40_RS17290 0.37 -1.3 3,458,145 - ABIE40_RS17290 0.37 +0.7 3,458,166 + ABIE40_RS17290 0.39 -2.7 3,458,166 + ABIE40_RS17290 0.39 +0.7 3,458,166 + ABIE40_RS17290 0.39 +0.2 3,458,167 - ABIE40_RS17290 0.39 -1.1 3,458,167 - ABIE40_RS17290 0.39 -1.3 3,458,238 + ABIE40_RS17290 0.47 -1.1 3,458,238 + ABIE40_RS17290 0.47 -0.3 3,458,238 + ABIE40_RS17290 0.47 -2.0 3,458,238 + ABIE40_RS17290 0.47 +0.7 3,458,293 - ABIE40_RS17290 0.54 -0.9 3,458,358 + ABIE40_RS17290 0.61 -1.5 3,458,358 + ABIE40_RS17290 0.61 -2.1 3,458,359 - ABIE40_RS17290 0.61 -0.6 3,458,376 - ABIE40_RS17290 0.63 -0.0 3,458,544 + ABIE40_RS17290 0.82 -1.8 3,458,584 - ABIE40_RS17290 0.87 -0.8 3,458,584 - ABIE40_RS17290 0.87 +1.4
Or see this region's nucleotide sequence