Experiment: R2A with 3-octanone 10.6206 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS03780 and ABIE40_RS03785 are separated by 14 nucleotides ABIE40_RS03785 and ABIE40_RS03790 are separated by 61 nucleotides ABIE40_RS03790 and ABIE40_RS03795 are separated by 175 nucleotides
ABIE40_RS03780: ABIE40_RS03780 - TldD/PmbA family protein, at 747,384 to 748,730
_RS03780
ABIE40_RS03785: ABIE40_RS03785 - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 748,745 to 749,554
_RS03785
ABIE40_RS03790: ABIE40_RS03790 - DUF4170 domain-containing protein, at 749,616 to 749,864
_RS03790
ABIE40_RS03795: ABIE40_RS03795 - lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase, at 750,040 to 751,356
_RS03795
Position (kb) 749
750 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 748.661 kb on + strand at 748.674 kb on + strand at 748.674 kb on + strand at 748.675 kb on - strand at 748.833 kb on - strand, within ABIE40_RS03785 at 748.949 kb on + strand, within ABIE40_RS03785 at 748.949 kb on + strand, within ABIE40_RS03785 at 748.950 kb on - strand, within ABIE40_RS03785 at 748.950 kb on - strand, within ABIE40_RS03785 at 748.950 kb on - strand, within ABIE40_RS03785 at 749.117 kb on + strand, within ABIE40_RS03785 at 749.117 kb on + strand, within ABIE40_RS03785 at 749.118 kb on - strand, within ABIE40_RS03785 at 749.312 kb on + strand, within ABIE40_RS03785 at 749.312 kb on + strand, within ABIE40_RS03785 at 749.312 kb on + strand, within ABIE40_RS03785 at 749.312 kb on + strand, within ABIE40_RS03785 at 749.312 kb on + strand, within ABIE40_RS03785 at 749.343 kb on + strand, within ABIE40_RS03785 at 749.344 kb on - strand, within ABIE40_RS03785 at 749.456 kb on + strand, within ABIE40_RS03785 at 749.456 kb on + strand, within ABIE40_RS03785 at 749.456 kb on + strand, within ABIE40_RS03785 at 749.456 kb on + strand, within ABIE40_RS03785 at 749.457 kb on - strand, within ABIE40_RS03785 at 749.542 kb on - strand at 749.634 kb on - strand at 749.634 kb on - strand at 749.634 kb on - strand at 749.740 kb on - strand, within ABIE40_RS03790 at 749.789 kb on + strand, within ABIE40_RS03790 at 749.798 kb on + strand, within ABIE40_RS03790 at 749.798 kb on + strand, within ABIE40_RS03790 at 749.798 kb on + strand, within ABIE40_RS03790 at 749.798 kb on + strand, within ABIE40_RS03790 at 749.798 kb on + strand, within ABIE40_RS03790 at 749.798 kb on + strand, within ABIE40_RS03790 at 749.798 kb on + strand, within ABIE40_RS03790 at 749.798 kb on + strand, within ABIE40_RS03790 at 749.798 kb on + strand, within ABIE40_RS03790 at 749.798 kb on + strand, within ABIE40_RS03790 at 749.798 kb on + strand, within ABIE40_RS03790 at 749.799 kb on - strand, within ABIE40_RS03790 at 749.799 kb on - strand, within ABIE40_RS03790 at 749.799 kb on - strand, within ABIE40_RS03790 at 749.805 kb on + strand, within ABIE40_RS03790 at 749.805 kb on + strand, within ABIE40_RS03790 at 749.806 kb on - strand, within ABIE40_RS03790 at 749.846 kb on + strand at 749.989 kb on + strand at 749.989 kb on + strand at 749.989 kb on + strand at 750.011 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 10.6206 mM remove 748,661 + -1.3 748,674 + +1.8 748,674 + +0.6 748,675 - -3.3 748,833 - ABIE40_RS03785 0.11 +0.5 748,949 + ABIE40_RS03785 0.25 +2.1 748,949 + ABIE40_RS03785 0.25 -0.6 748,950 - ABIE40_RS03785 0.25 -0.8 748,950 - ABIE40_RS03785 0.25 -1.6 748,950 - ABIE40_RS03785 0.25 -1.2 749,117 + ABIE40_RS03785 0.46 -0.2 749,117 + ABIE40_RS03785 0.46 +0.1 749,118 - ABIE40_RS03785 0.46 +0.6 749,312 + ABIE40_RS03785 0.70 +0.3 749,312 + ABIE40_RS03785 0.70 +1.0 749,312 + ABIE40_RS03785 0.70 +0.8 749,312 + ABIE40_RS03785 0.70 -0.0 749,312 + ABIE40_RS03785 0.70 +0.9 749,343 + ABIE40_RS03785 0.74 -3.5 749,344 - ABIE40_RS03785 0.74 -0.1 749,456 + ABIE40_RS03785 0.88 +0.0 749,456 + ABIE40_RS03785 0.88 +0.1 749,456 + ABIE40_RS03785 0.88 +0.2 749,456 + ABIE40_RS03785 0.88 -2.6 749,457 - ABIE40_RS03785 0.88 -2.7 749,542 - +0.6 749,634 - +0.8 749,634 - +0.2 749,634 - -0.2 749,740 - ABIE40_RS03790 0.50 +0.2 749,789 + ABIE40_RS03790 0.69 -0.8 749,798 + ABIE40_RS03790 0.73 -1.3 749,798 + ABIE40_RS03790 0.73 -2.0 749,798 + ABIE40_RS03790 0.73 -3.2 749,798 + ABIE40_RS03790 0.73 +1.8 749,798 + ABIE40_RS03790 0.73 -1.7 749,798 + ABIE40_RS03790 0.73 +1.5 749,798 + ABIE40_RS03790 0.73 +0.2 749,798 + ABIE40_RS03790 0.73 -0.2 749,798 + ABIE40_RS03790 0.73 -3.1 749,798 + ABIE40_RS03790 0.73 -0.9 749,798 + ABIE40_RS03790 0.73 -1.5 749,799 - ABIE40_RS03790 0.73 -0.5 749,799 - ABIE40_RS03790 0.73 +0.8 749,799 - ABIE40_RS03790 0.73 +1.8 749,805 + ABIE40_RS03790 0.76 -2.6 749,805 + ABIE40_RS03790 0.76 -2.3 749,806 - ABIE40_RS03790 0.76 -0.5 749,846 + +1.5 749,989 + -0.4 749,989 + -0.0 749,989 + -0.6 750,011 - -2.7
Or see this region's nucleotide sequence