Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.835572666666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS17725 and ABIE40_RS17730 are separated by 354 nucleotides ABIE40_RS17730 and ABIE40_RS17735 are separated by 125 nucleotides ABIE40_RS17735 and ABIE40_RS17740 are separated by 0 nucleotides ABIE40_RS17740 and ABIE40_RS17745 are separated by 110 nucleotides
ABIE40_RS17725: ABIE40_RS17725 - DUF992 domain-containing protein, at 3,537,686 to 3,538,201
_RS17725
ABIE40_RS17730: ABIE40_RS17730 - hypothetical protein, at 3,538,556 to 3,538,774
_RS17730
ABIE40_RS17735: ABIE40_RS17735 - hypothetical protein, at 3,538,900 to 3,539,064
_RS17735
ABIE40_RS17740: ABIE40_RS17740 - class I SAM-dependent methyltransferase, at 3,539,065 to 3,539,862
_RS17740
ABIE40_RS17745: ABIE40_RS17745 - BON domain-containing protein, at 3,539,973 to 3,540,239
_RS17745
Position (kb)
3538
3539
3540 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3537.925 kb on + strand, within ABIE40_RS17725 at 3537.926 kb on - strand, within ABIE40_RS17725 at 3537.964 kb on + strand, within ABIE40_RS17725 at 3537.966 kb on + strand, within ABIE40_RS17725 at 3537.966 kb on + strand, within ABIE40_RS17725 at 3537.966 kb on + strand, within ABIE40_RS17725 at 3537.967 kb on - strand, within ABIE40_RS17725 at 3537.967 kb on - strand, within ABIE40_RS17725 at 3537.967 kb on - strand, within ABIE40_RS17725 at 3538.039 kb on - strand, within ABIE40_RS17725 at 3538.039 kb on - strand, within ABIE40_RS17725 at 3538.096 kb on + strand, within ABIE40_RS17725 at 3538.110 kb on + strand, within ABIE40_RS17725 at 3538.110 kb on + strand, within ABIE40_RS17725 at 3538.111 kb on - strand, within ABIE40_RS17725 at 3538.111 kb on - strand, within ABIE40_RS17725 at 3538.111 kb on - strand, within ABIE40_RS17725 at 3538.124 kb on - strand, within ABIE40_RS17725 at 3538.178 kb on + strand at 3538.179 kb on - strand at 3538.179 kb on - strand at 3538.179 kb on - strand at 3538.179 kb on - strand at 3538.179 kb on - strand at 3538.275 kb on + strand at 3538.275 kb on + strand at 3538.275 kb on + strand at 3538.275 kb on + strand at 3538.276 kb on - strand at 3538.276 kb on - strand at 3538.276 kb on - strand at 3538.280 kb on + strand at 3538.280 kb on + strand at 3538.281 kb on - strand at 3538.320 kb on + strand at 3538.321 kb on - strand at 3538.321 kb on - strand at 3538.321 kb on - strand at 3538.321 kb on - strand at 3538.321 kb on - strand at 3538.343 kb on - strand at 3538.343 kb on - strand at 3538.459 kb on + strand at 3538.486 kb on + strand at 3538.537 kb on + strand at 3538.567 kb on + strand at 3538.567 kb on + strand at 3538.567 kb on + strand at 3538.568 kb on - strand at 3538.568 kb on - strand at 3538.568 kb on - strand at 3538.568 kb on - strand at 3538.706 kb on + strand, within ABIE40_RS17730 at 3538.706 kb on + strand, within ABIE40_RS17730 at 3538.706 kb on + strand, within ABIE40_RS17730 at 3538.706 kb on + strand, within ABIE40_RS17730 at 3538.706 kb on + strand, within ABIE40_RS17730 at 3538.706 kb on + strand, within ABIE40_RS17730 at 3538.706 kb on + strand, within ABIE40_RS17730 at 3538.706 kb on + strand, within ABIE40_RS17730 at 3538.780 kb on - strand at 3538.874 kb on + strand at 3538.875 kb on - strand at 3538.875 kb on - strand at 3538.933 kb on + strand, within ABIE40_RS17735 at 3538.933 kb on + strand, within ABIE40_RS17735 at 3538.934 kb on - strand, within ABIE40_RS17735 at 3538.934 kb on - strand, within ABIE40_RS17735 at 3538.934 kb on - strand, within ABIE40_RS17735 at 3538.934 kb on - strand, within ABIE40_RS17735 at 3538.934 kb on - strand, within ABIE40_RS17735 at 3538.934 kb on - strand, within ABIE40_RS17735 at 3539.082 kb on + strand at 3539.083 kb on - strand at 3539.085 kb on - strand at 3539.085 kb on - strand at 3539.102 kb on + strand at 3539.102 kb on + strand at 3539.102 kb on + strand at 3539.103 kb on - strand at 3539.103 kb on - strand at 3539.222 kb on + strand, within ABIE40_RS17740 at 3539.222 kb on + strand, within ABIE40_RS17740 at 3539.222 kb on + strand, within ABIE40_RS17740 at 3539.333 kb on - strand, within ABIE40_RS17740 at 3539.333 kb on - strand, within ABIE40_RS17740 at 3539.333 kb on - strand, within ABIE40_RS17740 at 3539.376 kb on + strand, within ABIE40_RS17740 at 3539.376 kb on + strand, within ABIE40_RS17740 at 3539.377 kb on - strand, within ABIE40_RS17740 at 3539.467 kb on + strand, within ABIE40_RS17740 at 3539.467 kb on + strand, within ABIE40_RS17740 at 3539.468 kb on - strand, within ABIE40_RS17740 at 3539.522 kb on + strand, within ABIE40_RS17740 at 3539.522 kb on + strand, within ABIE40_RS17740 at 3539.523 kb on - strand, within ABIE40_RS17740 at 3539.606 kb on + strand, within ABIE40_RS17740 at 3539.640 kb on + strand, within ABIE40_RS17740 at 3539.640 kb on + strand, within ABIE40_RS17740 at 3539.640 kb on + strand, within ABIE40_RS17740 at 3539.641 kb on - strand, within ABIE40_RS17740 at 3539.641 kb on - strand, within ABIE40_RS17740 at 3539.641 kb on - strand, within ABIE40_RS17740 at 3539.641 kb on - strand, within ABIE40_RS17740 at 3539.641 kb on - strand, within ABIE40_RS17740 at 3539.641 kb on - strand, within ABIE40_RS17740 at 3539.642 kb on + strand, within ABIE40_RS17740 at 3539.643 kb on - strand, within ABIE40_RS17740 at 3539.657 kb on + strand, within ABIE40_RS17740 at 3539.657 kb on + strand, within ABIE40_RS17740 at 3539.657 kb on + strand, within ABIE40_RS17740 at 3539.657 kb on + strand, within ABIE40_RS17740 at 3539.658 kb on - strand, within ABIE40_RS17740 at 3539.658 kb on - strand, within ABIE40_RS17740 at 3539.658 kb on - strand, within ABIE40_RS17740 at 3539.801 kb on + strand at 3539.899 kb on - strand at 3539.904 kb on + strand at 3539.904 kb on + strand at 3539.931 kb on + strand at 3539.931 kb on + strand at 3539.932 kb on - strand at 3539.941 kb on + strand at 3539.953 kb on - strand at 3539.959 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.835572666666667 mM remove 3,537,925 + ABIE40_RS17725 0.46 +0.2 3,537,926 - ABIE40_RS17725 0.47 -0.3 3,537,964 + ABIE40_RS17725 0.54 +0.6 3,537,966 + ABIE40_RS17725 0.54 -1.1 3,537,966 + ABIE40_RS17725 0.54 -1.0 3,537,966 + ABIE40_RS17725 0.54 -0.7 3,537,967 - ABIE40_RS17725 0.54 -0.4 3,537,967 - ABIE40_RS17725 0.54 -0.0 3,537,967 - ABIE40_RS17725 0.54 +0.7 3,538,039 - ABIE40_RS17725 0.68 -1.9 3,538,039 - ABIE40_RS17725 0.68 -1.5 3,538,096 + ABIE40_RS17725 0.79 -0.4 3,538,110 + ABIE40_RS17725 0.82 -0.4 3,538,110 + ABIE40_RS17725 0.82 -1.3 3,538,111 - ABIE40_RS17725 0.82 -0.4 3,538,111 - ABIE40_RS17725 0.82 -0.9 3,538,111 - ABIE40_RS17725 0.82 +1.2 3,538,124 - ABIE40_RS17725 0.85 -1.6 3,538,178 + -1.2 3,538,179 - -1.7 3,538,179 - -1.1 3,538,179 - +0.4 3,538,179 - -2.0 3,538,179 - +1.6 3,538,275 + -2.4 3,538,275 + -0.6 3,538,275 + +0.2 3,538,275 + -1.2 3,538,276 - -0.4 3,538,276 - -0.0 3,538,276 - -0.2 3,538,280 + -1.4 3,538,280 + -2.8 3,538,281 - +0.6 3,538,320 + -0.8 3,538,321 - +0.1 3,538,321 - +0.4 3,538,321 - +0.5 3,538,321 - +0.3 3,538,321 - -0.2 3,538,343 - +0.1 3,538,343 - +0.9 3,538,459 + +0.6 3,538,486 + -0.2 3,538,537 + +0.7 3,538,567 + -0.2 3,538,567 + +0.4 3,538,567 + -0.4 3,538,568 - -1.2 3,538,568 - +1.2 3,538,568 - -2.0 3,538,568 - -1.9 3,538,706 + ABIE40_RS17730 0.68 +0.8 3,538,706 + ABIE40_RS17730 0.68 +1.4 3,538,706 + ABIE40_RS17730 0.68 +0.1 3,538,706 + ABIE40_RS17730 0.68 +0.4 3,538,706 + ABIE40_RS17730 0.68 +0.3 3,538,706 + ABIE40_RS17730 0.68 +1.0 3,538,706 + ABIE40_RS17730 0.68 +0.8 3,538,706 + ABIE40_RS17730 0.68 +0.2 3,538,780 - -0.1 3,538,874 + -0.3 3,538,875 - +1.0 3,538,875 - +0.9 3,538,933 + ABIE40_RS17735 0.20 -0.1 3,538,933 + ABIE40_RS17735 0.20 -1.6 3,538,934 - ABIE40_RS17735 0.21 -0.2 3,538,934 - ABIE40_RS17735 0.21 +0.5 3,538,934 - ABIE40_RS17735 0.21 +0.6 3,538,934 - ABIE40_RS17735 0.21 +0.4 3,538,934 - ABIE40_RS17735 0.21 +0.8 3,538,934 - ABIE40_RS17735 0.21 +1.2 3,539,082 + +0.5 3,539,083 - -0.2 3,539,085 - +0.3 3,539,085 - +0.5 3,539,102 + +0.1 3,539,102 + -2.1 3,539,102 + -1.8 3,539,103 - -0.8 3,539,103 - -0.6 3,539,222 + ABIE40_RS17740 0.20 -0.3 3,539,222 + ABIE40_RS17740 0.20 +0.3 3,539,222 + ABIE40_RS17740 0.20 -0.5 3,539,333 - ABIE40_RS17740 0.34 -0.1 3,539,333 - ABIE40_RS17740 0.34 +0.1 3,539,333 - ABIE40_RS17740 0.34 -0.3 3,539,376 + ABIE40_RS17740 0.39 +0.1 3,539,376 + ABIE40_RS17740 0.39 +0.4 3,539,377 - ABIE40_RS17740 0.39 +0.2 3,539,467 + ABIE40_RS17740 0.50 +0.7 3,539,467 + ABIE40_RS17740 0.50 +0.8 3,539,468 - ABIE40_RS17740 0.51 +0.2 3,539,522 + ABIE40_RS17740 0.57 -1.7 3,539,522 + ABIE40_RS17740 0.57 -0.1 3,539,523 - ABIE40_RS17740 0.57 -1.2 3,539,606 + ABIE40_RS17740 0.68 -1.0 3,539,640 + ABIE40_RS17740 0.72 -0.6 3,539,640 + ABIE40_RS17740 0.72 -0.4 3,539,640 + ABIE40_RS17740 0.72 +0.8 3,539,641 - ABIE40_RS17740 0.72 -0.9 3,539,641 - ABIE40_RS17740 0.72 -0.5 3,539,641 - ABIE40_RS17740 0.72 -0.8 3,539,641 - ABIE40_RS17740 0.72 -0.3 3,539,641 - ABIE40_RS17740 0.72 +0.7 3,539,641 - ABIE40_RS17740 0.72 +0.2 3,539,642 + ABIE40_RS17740 0.72 -0.8 3,539,643 - ABIE40_RS17740 0.72 +0.0 3,539,657 + ABIE40_RS17740 0.74 -0.8 3,539,657 + ABIE40_RS17740 0.74 -2.6 3,539,657 + ABIE40_RS17740 0.74 +2.8 3,539,657 + ABIE40_RS17740 0.74 -0.1 3,539,658 - ABIE40_RS17740 0.74 -1.1 3,539,658 - ABIE40_RS17740 0.74 +0.9 3,539,658 - ABIE40_RS17740 0.74 -0.2 3,539,801 + -1.1 3,539,899 - +0.7 3,539,904 + -1.1 3,539,904 + -0.1 3,539,931 + -0.4 3,539,931 + -0.4 3,539,932 - -1.9 3,539,941 + +0.1 3,539,953 - -1.1 3,539,959 + -0.4
Or see this region's nucleotide sequence