Experiment: Survival; Treatment=FW300; TreatmentDuration=2hrs; Outgrowth=R2A_Kan10_Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABCV34_RS09220 and ABCV34_RS09225 are separated by 57 nucleotides ABCV34_RS09225 and ABCV34_RS09230 are separated by 189 nucleotides ABCV34_RS09230 and ABCV34_RS09235 are separated by 111 nucleotides
ABCV34_RS09220: ABCV34_RS09220 - amidase, at 1,975,451 to 1,976,800
_RS09220
ABCV34_RS09225: ABCV34_RS09225 - CPBP family glutamic-type intramembrane protease, at 1,976,858 to 1,977,610
_RS09225
ABCV34_RS09230: ABCV34_RS09230 - aminoacyl-tRNA hydrolase, at 1,977,800 to 1,978,390
_RS09230
ABCV34_RS09235: ABCV34_RS09235 - 50S ribosomal protein L25/general stress protein Ctc, at 1,978,502 to 1,979,122
_RS09235
Position (kb)
1976
1977
1978 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1975.859 kb on + strand, within ABCV34_RS09220 at 1975.859 kb on + strand, within ABCV34_RS09220 at 1975.859 kb on + strand, within ABCV34_RS09220 at 1975.859 kb on + strand, within ABCV34_RS09220 at 1975.859 kb on + strand, within ABCV34_RS09220 at 1975.859 kb on + strand, within ABCV34_RS09220 at 1975.859 kb on + strand, within ABCV34_RS09220 at 1976.372 kb on + strand, within ABCV34_RS09220 at 1976.372 kb on + strand, within ABCV34_RS09220 at 1976.372 kb on + strand, within ABCV34_RS09220 at 1976.372 kb on + strand, within ABCV34_RS09220 at 1976.372 kb on + strand, within ABCV34_RS09220 at 1976.471 kb on + strand, within ABCV34_RS09220 at 1976.471 kb on + strand, within ABCV34_RS09220 at 1976.471 kb on + strand, within ABCV34_RS09220 at 1976.471 kb on + strand, within ABCV34_RS09220 at 1976.471 kb on + strand, within ABCV34_RS09220 at 1976.471 kb on + strand, within ABCV34_RS09220 at 1976.472 kb on - strand, within ABCV34_RS09220 at 1976.472 kb on - strand, within ABCV34_RS09220 at 1976.472 kb on - strand, within ABCV34_RS09220 at 1976.474 kb on - strand, within ABCV34_RS09220 at 1976.559 kb on + strand, within ABCV34_RS09220 at 1976.609 kb on + strand, within ABCV34_RS09220 at 1976.609 kb on + strand, within ABCV34_RS09220 at 1976.609 kb on + strand, within ABCV34_RS09220 at 1976.609 kb on + strand, within ABCV34_RS09220 at 1976.609 kb on + strand, within ABCV34_RS09220 at 1976.610 kb on - strand, within ABCV34_RS09220 at 1976.610 kb on - strand, within ABCV34_RS09220 at 1976.610 kb on - strand, within ABCV34_RS09220 at 1976.798 kb on + strand at 1976.809 kb on + strand at 1976.809 kb on + strand at 1976.809 kb on + strand at 1976.809 kb on + strand at 1976.809 kb on + strand at 1976.809 kb on + strand at 1976.810 kb on - strand at 1976.810 kb on - strand at 1976.810 kb on - strand at 1976.810 kb on - strand at 1976.811 kb on + strand at 1976.811 kb on + strand at 1976.811 kb on + strand at 1976.812 kb on - strand at 1976.862 kb on + strand at 1976.863 kb on - strand at 1976.863 kb on - strand at 1977.058 kb on + strand, within ABCV34_RS09225 at 1977.058 kb on + strand, within ABCV34_RS09225 at 1977.060 kb on + strand, within ABCV34_RS09225 at 1977.060 kb on + strand, within ABCV34_RS09225 at 1977.060 kb on + strand, within ABCV34_RS09225 at 1977.060 kb on + strand, within ABCV34_RS09225 at 1977.060 kb on + strand, within ABCV34_RS09225 at 1977.060 kb on + strand, within ABCV34_RS09225 at 1977.061 kb on - strand, within ABCV34_RS09225 at 1977.061 kb on - strand, within ABCV34_RS09225 at 1977.111 kb on + strand, within ABCV34_RS09225 at 1977.184 kb on - strand, within ABCV34_RS09225 at 1977.209 kb on + strand, within ABCV34_RS09225 at 1977.635 kb on + strand at 1977.635 kb on + strand at 1977.636 kb on - strand at 1977.636 kb on - strand at 1977.801 kb on + strand at 1977.801 kb on + strand at 1977.802 kb on - strand at 1977.802 kb on - strand at 1978.418 kb on + strand at 1978.418 kb on + strand at 1978.419 kb on - strand at 1978.419 kb on - strand at 1978.504 kb on - strand at 1978.504 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300; TreatmentDuration=2hrs; Outgrowth=R2A_Kan10_Lactate remove 1,975,859 + ABCV34_RS09220 0.30 -2.5 1,975,859 + ABCV34_RS09220 0.30 -0.4 1,975,859 + ABCV34_RS09220 0.30 +0.6 1,975,859 + ABCV34_RS09220 0.30 -1.5 1,975,859 + ABCV34_RS09220 0.30 +0.3 1,975,859 + ABCV34_RS09220 0.30 -0.6 1,975,859 + ABCV34_RS09220 0.30 -1.3 1,976,372 + ABCV34_RS09220 0.68 -3.2 1,976,372 + ABCV34_RS09220 0.68 -2.1 1,976,372 + ABCV34_RS09220 0.68 -0.4 1,976,372 + ABCV34_RS09220 0.68 -0.7 1,976,372 + ABCV34_RS09220 0.68 -1.8 1,976,471 + ABCV34_RS09220 0.76 +0.2 1,976,471 + ABCV34_RS09220 0.76 -1.1 1,976,471 + ABCV34_RS09220 0.76 -0.7 1,976,471 + ABCV34_RS09220 0.76 -1.1 1,976,471 + ABCV34_RS09220 0.76 -0.6 1,976,471 + ABCV34_RS09220 0.76 -1.4 1,976,472 - ABCV34_RS09220 0.76 -0.0 1,976,472 - ABCV34_RS09220 0.76 -1.8 1,976,472 - ABCV34_RS09220 0.76 -1.9 1,976,474 - ABCV34_RS09220 0.76 -1.1 1,976,559 + ABCV34_RS09220 0.82 -0.4 1,976,609 + ABCV34_RS09220 0.86 +1.4 1,976,609 + ABCV34_RS09220 0.86 -0.4 1,976,609 + ABCV34_RS09220 0.86 -0.9 1,976,609 + ABCV34_RS09220 0.86 -0.8 1,976,609 + ABCV34_RS09220 0.86 -0.5 1,976,610 - ABCV34_RS09220 0.86 +1.9 1,976,610 - ABCV34_RS09220 0.86 +0.9 1,976,610 - ABCV34_RS09220 0.86 +0.2 1,976,798 + -2.2 1,976,809 + -2.1 1,976,809 + -1.3 1,976,809 + -2.3 1,976,809 + -2.3 1,976,809 + -2.4 1,976,809 + -2.9 1,976,810 - -0.6 1,976,810 - -1.3 1,976,810 - -0.4 1,976,810 - -0.3 1,976,811 + -1.2 1,976,811 + -1.4 1,976,811 + -1.2 1,976,812 - -0.6 1,976,862 + -1.7 1,976,863 - +0.3 1,976,863 - -1.6 1,977,058 + ABCV34_RS09225 0.27 -2.6 1,977,058 + ABCV34_RS09225 0.27 -1.4 1,977,060 + ABCV34_RS09225 0.27 -1.6 1,977,060 + ABCV34_RS09225 0.27 +0.6 1,977,060 + ABCV34_RS09225 0.27 -1.6 1,977,060 + ABCV34_RS09225 0.27 -0.4 1,977,060 + ABCV34_RS09225 0.27 +1.9 1,977,060 + ABCV34_RS09225 0.27 -1.1 1,977,061 - ABCV34_RS09225 0.27 -1.3 1,977,061 - ABCV34_RS09225 0.27 -1.6 1,977,111 + ABCV34_RS09225 0.34 -2.5 1,977,184 - ABCV34_RS09225 0.43 -1.0 1,977,209 + ABCV34_RS09225 0.47 -1.0 1,977,635 + -2.7 1,977,635 + +1.6 1,977,636 - +1.4 1,977,636 - -0.6 1,977,801 + -1.4 1,977,801 + -0.6 1,977,802 - +1.0 1,977,802 - -0.8 1,978,418 + -1.5 1,978,418 + -0.6 1,978,419 - -0.7 1,978,419 - +0.3 1,978,504 - -0.6 1,978,504 - -0.7
Or see this region's nucleotide sequence