Experiment: A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_01430 and AO353_01435 overlap by 4 nucleotides AO353_01435 and AO353_01440 overlap by 1 nucleotides AO353_01440 and AO353_01445 overlap by 4 nucleotides AO353_01445 and AO353_01450 are separated by 24 nucleotides
AO353_01430: AO353_01430 - UDP-N-acetylenolpyruvoylglucosamine reductase, at 308,605 to 309,624
_01430
AO353_01435: AO353_01435 - protein tyrosine phosphatase, at 309,621 to 310,085
_01435
AO353_01440: AO353_01440 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 310,085 to 310,849
_01440
AO353_01445: AO353_01445 - hypothetical protein, at 310,846 to 311,031
_01445
AO353_01450: AO353_01450 - tetraacyldisaccharide 4'-kinase, at 311,056 to 312,066
_01450
Position (kb) 309
310
311 Strain fitness (log2 ratio) -2
-1
0
1 at 309.625 kb on - strand at 309.710 kb on - strand, within AO353_01435 at 309.710 kb on - strand, within AO353_01435 at 309.710 kb on - strand, within AO353_01435 at 309.710 kb on - strand, within AO353_01435 at 309.713 kb on - strand, within AO353_01435 at 309.713 kb on - strand, within AO353_01435 at 309.746 kb on - strand, within AO353_01435 at 309.845 kb on - strand, within AO353_01435 at 309.845 kb on - strand, within AO353_01435 at 309.899 kb on - strand, within AO353_01435 at 311.058 kb on - strand at 311.058 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.01 mg/ml remove 309,625 - +0.1 309,710 - AO353_01435 0.19 -0.2 309,710 - AO353_01435 0.19 -0.3 309,710 - AO353_01435 0.19 -0.2 309,710 - AO353_01435 0.19 -0.4 309,713 - AO353_01435 0.20 -2.3 309,713 - AO353_01435 0.20 +1.2 309,746 - AO353_01435 0.27 -1.3 309,845 - AO353_01435 0.48 -1.4 309,845 - AO353_01435 0.48 -0.4 309,899 - AO353_01435 0.60 -1.9 311,058 - -0.1 311,058 - -0.2
Or see this region's nucleotide sequence