Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF794 and GFF795 are separated by 2 nucleotides GFF795 and GFF796 overlap by 4 nucleotides GFF796 and GFF797 are separated by 291 nucleotides GFF797 and GFF798 are separated by 75 nucleotides GFF798 and GFF799 are separated by 51 nucleotides
GFF794 - Phage holin, at 143,876 to 144,220
GFF794
GFF795 - Phage lysin, 1,4-beta-N-acetylmuramidase (EC 3.2.1.17) or lysozyme, at 144,223 to 144,837
GFF795
GFF796 - Phage outer membrane lytic protein Rz; Endopeptidase (EC 3.4.-.-), at 144,834 to 145,319
GFF796
GFF797 - hypothetical protein, at 145,611 to 145,802
GFF797
GFF798 - FIG01046582: hypothetical protein, at 145,878 to 146,102
GFF798
GFF799 - FIG01045807: hypothetical protein, at 146,154 to 146,645
GFF799
Position (kb) 144
145
146 Strain fitness (log2 ratio) -1
0
1 at 143.950 kb on - strand, within GFF794 at 144.003 kb on - strand, within GFF794 at 144.477 kb on + strand, within GFF795 at 144.477 kb on + strand, within GFF795 at 144.478 kb on - strand, within GFF795 at 144.588 kb on + strand, within GFF795 at 144.733 kb on + strand, within GFF795 at 144.850 kb on + strand at 144.850 kb on + strand at 145.001 kb on + strand, within GFF796 at 145.001 kb on + strand, within GFF796 at 145.001 kb on + strand, within GFF796 at 145.002 kb on - strand, within GFF796 at 145.051 kb on - strand, within GFF796 at 145.293 kb on + strand at 145.354 kb on + strand at 145.422 kb on + strand at 145.454 kb on - strand at 145.531 kb on + strand at 145.545 kb on - strand at 145.594 kb on - strand at 145.598 kb on + strand at 145.599 kb on - strand at 145.788 kb on - strand at 145.861 kb on - strand at 145.879 kb on + strand at 145.913 kb on - strand, within GFF798 at 145.929 kb on - strand, within GFF798 at 146.290 kb on - strand, within GFF799 at 146.290 kb on - strand, within GFF799
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 143,950 - GFF794 0.21 +0.5 144,003 - GFF794 0.37 -1.1 144,477 + GFF795 0.41 +0.1 144,477 + GFF795 0.41 -0.0 144,478 - GFF795 0.41 +0.6 144,588 + GFF795 0.59 -0.3 144,733 + GFF795 0.83 +0.8 144,850 + +0.0 144,850 + -0.6 145,001 + GFF796 0.34 -0.7 145,001 + GFF796 0.34 -0.6 145,001 + GFF796 0.34 -0.4 145,002 - GFF796 0.35 -1.0 145,051 - GFF796 0.45 -0.6 145,293 + -0.3 145,354 + -0.7 145,422 + +0.5 145,454 - +0.5 145,531 + -0.7 145,545 - -0.2 145,594 - +1.4 145,598 + -0.3 145,599 - -0.6 145,788 - -0.3 145,861 - +0.2 145,879 + -0.3 145,913 - GFF798 0.16 +0.7 145,929 - GFF798 0.23 +0.1 146,290 - GFF799 0.28 +0.2 146,290 - GFF799 0.28 -0.3
Or see this region's nucleotide sequence