Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF775 and GFF776 are separated by 7 nucleotides GFF776 and GFF777 are separated by 205 nucleotides GFF777 and GFF778 are separated by 217 nucleotides GFF778 and GFF779 overlap by 4 nucleotides GFF779 and GFF780 are separated by 11 nucleotides GFF780 and GFF781 overlap by 11 nucleotides GFF781 and GFF782 overlap by 1 nucleotides
GFF775 - putative excisionase, at 131,029 to 131,271
GFF775
GFF776 - Phage protein, at 131,279 to 131,431
GFF776
GFF777 - COG0419: ATPase involved in DNA repair, at 131,637 to 131,945
GFF777
GFF778 - Mobile element protein, at 132,163 to 132,408
GFF778
GFF779 - Ant protein, at 132,405 to 132,740
GFF779
GFF780 - FIG00643896: hypothetical protein, at 132,752 to 133,147
GFF780
GFF781 - Phage protein, at 133,137 to 133,373
GFF781
GFF782 - hypothetical protein, at 133,373 to 133,732
GFF782
Position (kb) 132
133 Strain fitness (log2 ratio) -1
0
1
2 at 131.249 kb on - strand at 131.255 kb on + strand at 131.260 kb on - strand at 131.467 kb on + strand at 131.469 kb on + strand at 131.470 kb on - strand at 131.483 kb on + strand at 131.584 kb on - strand at 131.639 kb on - strand at 131.639 kb on - strand at 131.708 kb on - strand, within GFF777 at 131.839 kb on + strand, within GFF777 at 131.910 kb on + strand, within GFF777 at 131.922 kb on - strand at 132.047 kb on + strand at 132.048 kb on - strand at 132.236 kb on + strand, within GFF778 at 132.246 kb on + strand, within GFF778 at 132.721 kb on - strand at 133.094 kb on - strand, within GFF780 at 133.094 kb on - strand, within GFF780 at 133.193 kb on + strand, within GFF781 at 133.246 kb on - strand, within GFF781
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 131,249 - -0.2 131,255 + +0.3 131,260 - -0.5 131,467 + -0.2 131,469 + -0.8 131,470 - -0.8 131,483 + +0.4 131,584 - +0.1 131,639 - -0.5 131,639 - +0.7 131,708 - GFF777 0.23 +1.0 131,839 + GFF777 0.65 +0.1 131,910 + GFF777 0.88 +0.0 131,922 - +2.6 132,047 + -0.4 132,048 - -0.9 132,236 + GFF778 0.30 -0.2 132,246 + GFF778 0.34 -1.1 132,721 - -1.4 133,094 - GFF780 0.86 -0.5 133,094 - GFF780 0.86 +0.3 133,193 + GFF781 0.24 +0.1 133,246 - GFF781 0.46 -0.6
Or see this region's nucleotide sequence