Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3313 and GFF3314 are separated by 83 nucleotides GFF3314 and GFF3315 are separated by 23 nucleotides GFF3315 and GFF3316 are separated by 88 nucleotides GFF3316 and GFF3317 are separated by 38 nucleotides
GFF3313 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 87,117 to 88,055
GFF3313
GFF3314 - Putative hydrolase YcdX (EC 3.1.-.-), at 88,139 to 88,876
GFF3314
GFF3315 - Putative oxidoreductase component of anaerobic dehydrogenases; Functional role page for Chaperone protein TorD, at 88,900 to 89,454
GFF3315
GFF3316 - Inner membrane protein YcdZ, at 89,543 to 90,037
GFF3316
GFF3317 - Curli production assembly/transport component CsgG, at 90,076 to 90,909
GFF3317
Position (kb) 88
89
90 Strain fitness (log2 ratio) -1
0
1 at 88.056 kb on + strand at 88.056 kb on + strand at 88.057 kb on - strand at 88.057 kb on - strand at 88.100 kb on - strand at 88.160 kb on - strand at 88.166 kb on - strand at 88.188 kb on - strand at 88.193 kb on - strand at 88.205 kb on + strand at 88.207 kb on + strand at 88.521 kb on + strand, within GFF3314 at 88.521 kb on + strand, within GFF3314 at 88.522 kb on - strand, within GFF3314 at 88.522 kb on - strand, within GFF3314 at 88.526 kb on - strand, within GFF3314 at 88.526 kb on - strand, within GFF3314 at 88.562 kb on - strand, within GFF3314 at 88.874 kb on + strand at 88.875 kb on - strand at 89.334 kb on + strand, within GFF3315 at 89.452 kb on + strand at 89.463 kb on - strand at 89.573 kb on - strand at 89.573 kb on - strand at 89.637 kb on + strand, within GFF3316 at 89.719 kb on + strand, within GFF3316 at 89.735 kb on - strand, within GFF3316 at 89.929 kb on - strand, within GFF3316 at 90.070 kb on + strand at 90.070 kb on + strand at 90.070 kb on + strand at 90.121 kb on - strand at 90.249 kb on - strand, within GFF3317 at 90.307 kb on + strand, within GFF3317 at 90.308 kb on - strand, within GFF3317 at 90.430 kb on - strand, within GFF3317
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 88,056 + +0.1 88,056 + -0.6 88,057 - +0.5 88,057 - +0.2 88,100 - -0.3 88,160 - -0.7 88,166 - +0.1 88,188 - +0.0 88,193 - +0.9 88,205 + -0.3 88,207 + +0.6 88,521 + GFF3314 0.52 +0.9 88,521 + GFF3314 0.52 -0.7 88,522 - GFF3314 0.52 +0.3 88,522 - GFF3314 0.52 -0.7 88,526 - GFF3314 0.52 -0.3 88,526 - GFF3314 0.52 -0.0 88,562 - GFF3314 0.57 +0.4 88,874 + -1.0 88,875 - +0.7 89,334 + GFF3315 0.78 -0.9 89,452 + +0.9 89,463 - +0.2 89,573 - -1.1 89,573 - -0.9 89,637 + GFF3316 0.19 +0.2 89,719 + GFF3316 0.36 -1.3 89,735 - GFF3316 0.39 -0.2 89,929 - GFF3316 0.78 -0.3 90,070 + +0.0 90,070 + +0.1 90,070 + -1.1 90,121 - +0.4 90,249 - GFF3317 0.21 -1.4 90,307 + GFF3317 0.28 +0.4 90,308 - GFF3317 0.28 -1.1 90,430 - GFF3317 0.42 +0.0
Or see this region's nucleotide sequence