Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3603 and Shew_3604 are separated by 294 nucleotides Shew_3604 and Shew_3605 are separated by 14 nucleotides Shew_3605 and Shew_3606 are separated by 83 nucleotides
Shew_3603: Shew_3603 - RNA polymerase factor sigma-32 (RefSeq), at 4,301,446 to 4,302,303
_3603
Shew_3604: Shew_3604 - hypothetical protein (RefSeq), at 4,302,598 to 4,303,563
_3604
Shew_3605: Shew_3605 - cell division ATP-binding protein FtsE (RefSeq), at 4,303,578 to 4,304,279
_3605
Shew_3606: Shew_3606 - signal recognition particle-docking protein FtsY (RefSeq), at 4,304,363 to 4,306,048
_3606
Position (kb)
4302
4303
4304 Strain fitness (log2 ratio)
-2
-1
0
1 at 4302.592 kb on - strand at 4302.675 kb on - strand at 4302.824 kb on - strand, within Shew_3604 at 4303.133 kb on - strand, within Shew_3604 at 4303.176 kb on - strand, within Shew_3604 at 4303.176 kb on - strand, within Shew_3604 at 4303.177 kb on - strand, within Shew_3604 at 4303.281 kb on + strand, within Shew_3604 at 4303.286 kb on - strand, within Shew_3604 at 4303.350 kb on + strand, within Shew_3604 at 4303.406 kb on - strand, within Shew_3604 at 4303.406 kb on - strand, within Shew_3604 at 4303.469 kb on - strand at 4303.469 kb on - strand at 4303.569 kb on - strand at 4303.634 kb on + strand at 4303.646 kb on + strand at 4303.951 kb on - strand, within Shew_3605 at 4304.164 kb on + strand, within Shew_3605 at 4304.232 kb on + strand at 4304.253 kb on - strand at 4304.254 kb on + strand at 4304.337 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 4,302,592 - -0.6 4,302,675 - +0.0 4,302,824 - Shew_3604 0.23 +0.0 4,303,133 - Shew_3604 0.55 -1.2 4,303,176 - Shew_3604 0.60 -1.3 4,303,176 - Shew_3604 0.60 -0.0 4,303,177 - Shew_3604 0.60 -1.1 4,303,281 + Shew_3604 0.71 -0.3 4,303,286 - Shew_3604 0.71 -1.5 4,303,350 + Shew_3604 0.78 -0.7 4,303,406 - Shew_3604 0.84 -0.3 4,303,406 - Shew_3604 0.84 -0.1 4,303,469 - -0.6 4,303,469 - -0.6 4,303,569 - +1.0 4,303,634 + -0.3 4,303,646 + -1.8 4,303,951 - Shew_3605 0.53 -0.6 4,304,164 + Shew_3605 0.83 +0.3 4,304,232 + -0.5 4,304,253 - +0.0 4,304,254 + +0.1 4,304,337 + -1.4
Or see this region's nucleotide sequence