Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5129 and Pf6N2E2_6146 are separated by 415 nucleotides Pf6N2E2_6146 and Pf6N2E2_6147 are separated by 10 nucleotides Pf6N2E2_6147 and Pf6N2E2_6148 are separated by 8 nucleotides Pf6N2E2_6148 and Pf6N2E2_6149 are separated by 96 nucleotides Pf6N2E2_6149 and Pf6N2E2_5130 overlap by 9 nucleotides Pf6N2E2_5130 and Pf6N2E2_5131 are separated by 30 nucleotides Pf6N2E2_5131 and Pf6N2E2_5132 are separated by 139 nucleotides
Pf6N2E2_5129 - ClpB protein, at 5,913,135 to 5,915,699
_5129
Pf6N2E2_6146 - tRNA-Asn-GTT, at 5,916,115 to 5,916,187
_6146
Pf6N2E2_6147 - tRNA-Pro-TGG, at 5,916,198 to 5,916,271
_6147
Pf6N2E2_6148 - tRNA-Lys-TTT, at 5,916,280 to 5,916,352
_6148
Pf6N2E2_6149 - tRNA-Pro-TGG, at 5,916,449 to 5,916,522
_6149
Pf6N2E2_5130 - hypothetical protein, at 5,916,514 to 5,916,642
_5130
Pf6N2E2_5131 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 5,916,673 to 5,918,298
_5131
Pf6N2E2_5132 - NADH dehydrogenase (EC 1.6.99.3), at 5,918,438 to 5,919,736
_5132
Position (kb) 5916
5917
5918
5919 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5915.936 kb on + strand at 5915.936 kb on + strand at 5916.387 kb on + strand at 5916.526 kb on + strand at 5916.526 kb on + strand at 5916.526 kb on + strand at 5916.541 kb on + strand, within Pf6N2E2_5130 at 5916.541 kb on + strand, within Pf6N2E2_5130 at 5916.551 kb on + strand, within Pf6N2E2_5130 at 5916.657 kb on - strand at 5916.672 kb on + strand at 5916.913 kb on - strand, within Pf6N2E2_5131 at 5916.913 kb on - strand, within Pf6N2E2_5131 at 5916.922 kb on + strand, within Pf6N2E2_5131 at 5917.015 kb on + strand, within Pf6N2E2_5131 at 5917.015 kb on + strand, within Pf6N2E2_5131 at 5917.046 kb on - strand, within Pf6N2E2_5131 at 5917.225 kb on + strand, within Pf6N2E2_5131 at 5917.226 kb on - strand, within Pf6N2E2_5131 at 5917.311 kb on + strand, within Pf6N2E2_5131 at 5917.338 kb on + strand, within Pf6N2E2_5131 at 5917.339 kb on - strand, within Pf6N2E2_5131 at 5917.339 kb on - strand, within Pf6N2E2_5131 at 5918.034 kb on + strand, within Pf6N2E2_5131 at 5918.300 kb on + strand at 5918.300 kb on + strand at 5918.432 kb on - strand at 5918.432 kb on - strand at 5918.518 kb on - strand at 5918.518 kb on - strand at 5918.518 kb on - strand at 5918.542 kb on - strand at 5918.542 kb on - strand at 5918.553 kb on + strand at 5918.553 kb on + strand at 5918.553 kb on + strand at 5918.553 kb on + strand at 5918.554 kb on - strand at 5918.554 kb on - strand at 5918.655 kb on + strand, within Pf6N2E2_5132 at 5918.656 kb on - strand, within Pf6N2E2_5132 at 5918.664 kb on + strand, within Pf6N2E2_5132 at 5918.664 kb on + strand, within Pf6N2E2_5132 at 5918.664 kb on + strand, within Pf6N2E2_5132 at 5918.664 kb on + strand, within Pf6N2E2_5132 at 5918.665 kb on - strand, within Pf6N2E2_5132 at 5918.665 kb on - strand, within Pf6N2E2_5132 at 5918.665 kb on - strand, within Pf6N2E2_5132 at 5918.665 kb on - strand, within Pf6N2E2_5132 at 5918.665 kb on - strand, within Pf6N2E2_5132 at 5918.665 kb on - strand, within Pf6N2E2_5132 at 5918.842 kb on + strand, within Pf6N2E2_5132 at 5918.843 kb on - strand, within Pf6N2E2_5132 at 5919.062 kb on - strand, within Pf6N2E2_5132 at 5919.062 kb on - strand, within Pf6N2E2_5132 at 5919.173 kb on - strand, within Pf6N2E2_5132 at 5919.173 kb on - strand, within Pf6N2E2_5132 at 5919.173 kb on - strand, within Pf6N2E2_5132 at 5919.273 kb on + strand, within Pf6N2E2_5132
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,915,936 + -0.7 5,915,936 + +3.0 5,916,387 + +0.9 5,916,526 + +0.6 5,916,526 + +1.8 5,916,526 + -0.5 5,916,541 + Pf6N2E2_5130 0.21 +1.2 5,916,541 + Pf6N2E2_5130 0.21 +0.9 5,916,551 + Pf6N2E2_5130 0.29 +0.0 5,916,657 - +0.6 5,916,672 + +1.7 5,916,913 - Pf6N2E2_5131 0.15 +0.3 5,916,913 - Pf6N2E2_5131 0.15 +1.9 5,916,922 + Pf6N2E2_5131 0.15 -2.1 5,917,015 + Pf6N2E2_5131 0.21 -0.2 5,917,015 + Pf6N2E2_5131 0.21 +0.4 5,917,046 - Pf6N2E2_5131 0.23 +0.7 5,917,225 + Pf6N2E2_5131 0.34 -1.0 5,917,226 - Pf6N2E2_5131 0.34 +1.4 5,917,311 + Pf6N2E2_5131 0.39 -0.5 5,917,338 + Pf6N2E2_5131 0.41 +0.5 5,917,339 - Pf6N2E2_5131 0.41 -0.0 5,917,339 - Pf6N2E2_5131 0.41 +0.6 5,918,034 + Pf6N2E2_5131 0.84 +1.7 5,918,300 + +1.2 5,918,300 + +1.7 5,918,432 - -0.7 5,918,432 - -0.9 5,918,518 - +0.0 5,918,518 - +1.1 5,918,518 - +0.3 5,918,542 - +0.1 5,918,542 - -0.8 5,918,553 + -1.4 5,918,553 + -0.6 5,918,553 + +0.5 5,918,553 + -0.0 5,918,554 - +1.0 5,918,554 - +0.4 5,918,655 + Pf6N2E2_5132 0.17 +1.3 5,918,656 - Pf6N2E2_5132 0.17 -2.3 5,918,664 + Pf6N2E2_5132 0.17 -0.5 5,918,664 + Pf6N2E2_5132 0.17 -0.6 5,918,664 + Pf6N2E2_5132 0.17 -0.4 5,918,664 + Pf6N2E2_5132 0.17 -1.0 5,918,665 - Pf6N2E2_5132 0.17 +0.2 5,918,665 - Pf6N2E2_5132 0.17 +0.6 5,918,665 - Pf6N2E2_5132 0.17 -1.5 5,918,665 - Pf6N2E2_5132 0.17 +1.1 5,918,665 - Pf6N2E2_5132 0.17 +0.5 5,918,665 - Pf6N2E2_5132 0.17 +0.4 5,918,842 + Pf6N2E2_5132 0.31 -1.5 5,918,843 - Pf6N2E2_5132 0.31 -2.5 5,919,062 - Pf6N2E2_5132 0.48 -0.7 5,919,062 - Pf6N2E2_5132 0.48 -0.2 5,919,173 - Pf6N2E2_5132 0.57 -0.3 5,919,173 - Pf6N2E2_5132 0.57 -1.6 5,919,173 - Pf6N2E2_5132 0.57 -0.0 5,919,273 + Pf6N2E2_5132 0.64 +0.7
Or see this region's nucleotide sequence