Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4604

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4603 and Pf6N2E2_4604 are separated by 249 nucleotidesPf6N2E2_4604 and Pf6N2E2_4605 are separated by 123 nucleotides Pf6N2E2_4603 - NLP/P60 family protein, at 5,329,106 to 5,329,717 _4603 Pf6N2E2_4604 - FIG00955003: hypothetical protein, at 5,329,967 to 5,330,395 _4604 Pf6N2E2_4605 - Dihydroorotase (EC 3.5.2.3), at 5,330,519 to 5,331,790 _4605 Position (kb) 5329 5330 5331Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 5329.007 kb on + strandat 5329.007 kb on + strandat 5329.167 kb on + strandat 5329.167 kb on + strandat 5329.167 kb on + strandat 5329.167 kb on + strandat 5329.167 kb on + strandat 5329.167 kb on + strandat 5329.167 kb on + strandat 5329.168 kb on - strand, within Pf6N2E2_4603at 5329.168 kb on - strand, within Pf6N2E2_4603at 5329.168 kb on - strand, within Pf6N2E2_4603at 5329.168 kb on - strand, within Pf6N2E2_4603at 5329.257 kb on + strand, within Pf6N2E2_4603at 5329.257 kb on + strand, within Pf6N2E2_4603at 5329.258 kb on - strand, within Pf6N2E2_4603at 5329.258 kb on - strand, within Pf6N2E2_4603at 5329.258 kb on - strand, within Pf6N2E2_4603at 5329.258 kb on - strand, within Pf6N2E2_4603at 5329.258 kb on - strand, within Pf6N2E2_4603at 5329.258 kb on - strand, within Pf6N2E2_4603at 5329.258 kb on - strand, within Pf6N2E2_4603at 5329.407 kb on + strand, within Pf6N2E2_4603at 5329.407 kb on + strand, within Pf6N2E2_4603at 5329.408 kb on - strand, within Pf6N2E2_4603at 5329.408 kb on - strand, within Pf6N2E2_4603at 5329.438 kb on + strand, within Pf6N2E2_4603at 5329.438 kb on + strand, within Pf6N2E2_4603at 5329.439 kb on - strand, within Pf6N2E2_4603at 5329.439 kb on - strand, within Pf6N2E2_4603at 5329.465 kb on - strand, within Pf6N2E2_4603at 5329.465 kb on - strand, within Pf6N2E2_4603at 5329.478 kb on + strand, within Pf6N2E2_4603at 5329.479 kb on - strand, within Pf6N2E2_4603at 5329.617 kb on + strand, within Pf6N2E2_4603at 5329.617 kb on + strand, within Pf6N2E2_4603at 5329.676 kb on + strandat 5329.676 kb on + strandat 5329.676 kb on + strandat 5329.677 kb on - strandat 5329.677 kb on - strandat 5329.679 kb on + strandat 5329.679 kb on + strandat 5329.680 kb on - strandat 5329.718 kb on + strandat 5329.719 kb on - strandat 5329.731 kb on + strandat 5329.732 kb on - strandat 5329.751 kb on + strandat 5329.752 kb on - strandat 5329.944 kb on + strandat 5329.945 kb on - strandat 5329.945 kb on - strandat 5329.976 kb on + strandat 5330.021 kb on + strand, within Pf6N2E2_4604at 5330.021 kb on + strand, within Pf6N2E2_4604at 5330.063 kb on + strand, within Pf6N2E2_4604at 5330.064 kb on - strand, within Pf6N2E2_4604at 5330.066 kb on + strand, within Pf6N2E2_4604at 5330.066 kb on + strand, within Pf6N2E2_4604at 5330.247 kb on + strand, within Pf6N2E2_4604at 5330.248 kb on - strand, within Pf6N2E2_4604at 5330.248 kb on - strand, within Pf6N2E2_4604at 5330.258 kb on + strand, within Pf6N2E2_4604at 5330.258 kb on + strand, within Pf6N2E2_4604at 5330.258 kb on + strand, within Pf6N2E2_4604at 5330.258 kb on + strand, within Pf6N2E2_4604at 5330.258 kb on + strand, within Pf6N2E2_4604at 5330.258 kb on + strand, within Pf6N2E2_4604at 5330.258 kb on + strand, within Pf6N2E2_4604at 5330.258 kb on + strand, within Pf6N2E2_4604at 5330.259 kb on - strand, within Pf6N2E2_4604at 5330.259 kb on - strand, within Pf6N2E2_4604at 5330.259 kb on - strand, within Pf6N2E2_4604at 5330.259 kb on - strand, within Pf6N2E2_4604at 5330.259 kb on - strand, within Pf6N2E2_4604at 5330.288 kb on + strand, within Pf6N2E2_4604at 5330.288 kb on + strand, within Pf6N2E2_4604at 5330.288 kb on + strand, within Pf6N2E2_4604at 5330.289 kb on - strand, within Pf6N2E2_4604at 5330.289 kb on - strand, within Pf6N2E2_4604at 5330.289 kb on - strand, within Pf6N2E2_4604at 5330.289 kb on - strand, within Pf6N2E2_4604at 5330.289 kb on - strand, within Pf6N2E2_4604at 5330.327 kb on + strand, within Pf6N2E2_4604at 5330.327 kb on + strand, within Pf6N2E2_4604at 5330.327 kb on + strand, within Pf6N2E2_4604at 5330.327 kb on + strand, within Pf6N2E2_4604at 5330.328 kb on - strand, within Pf6N2E2_4604at 5330.365 kb on + strandat 5330.393 kb on + strandat 5330.394 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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5,329,007 + -3.1
5,329,007 + +0.3
5,329,167 + +1.4
5,329,167 + +0.6
5,329,167 + +1.7
5,329,167 + +0.5
5,329,167 + +0.5
5,329,167 + -0.9
5,329,167 + -0.5
5,329,168 - Pf6N2E2_4603 0.10 +0.5
5,329,168 - Pf6N2E2_4603 0.10 +0.4
5,329,168 - Pf6N2E2_4603 0.10 -0.1
5,329,168 - Pf6N2E2_4603 0.10 +3.0
5,329,257 + Pf6N2E2_4603 0.25 +0.8
5,329,257 + Pf6N2E2_4603 0.25 -1.9
5,329,258 - Pf6N2E2_4603 0.25 -0.3
5,329,258 - Pf6N2E2_4603 0.25 +0.9
5,329,258 - Pf6N2E2_4603 0.25 +0.1
5,329,258 - Pf6N2E2_4603 0.25 -0.7
5,329,258 - Pf6N2E2_4603 0.25 -0.7
5,329,258 - Pf6N2E2_4603 0.25 +0.3
5,329,258 - Pf6N2E2_4603 0.25 +0.1
5,329,407 + Pf6N2E2_4603 0.49 +0.8
5,329,407 + Pf6N2E2_4603 0.49 -0.1
5,329,408 - Pf6N2E2_4603 0.49 -1.0
5,329,408 - Pf6N2E2_4603 0.49 -1.2
5,329,438 + Pf6N2E2_4603 0.54 +0.2
5,329,438 + Pf6N2E2_4603 0.54 -0.2
5,329,439 - Pf6N2E2_4603 0.54 +0.9
5,329,439 - Pf6N2E2_4603 0.54 -0.7
5,329,465 - Pf6N2E2_4603 0.59 -0.4
5,329,465 - Pf6N2E2_4603 0.59 -0.3
5,329,478 + Pf6N2E2_4603 0.61 +0.5
5,329,479 - Pf6N2E2_4603 0.61 +1.0
5,329,617 + Pf6N2E2_4603 0.83 -1.0
5,329,617 + Pf6N2E2_4603 0.83 +1.2
5,329,676 + -1.7
5,329,676 + +0.5
5,329,676 + +0.3
5,329,677 - +0.4
5,329,677 - -1.9
5,329,679 + -3.5
5,329,679 + +0.3
5,329,680 - -0.5
5,329,718 + -1.4
5,329,719 - -1.6
5,329,731 + -0.5
5,329,732 - -1.8
5,329,751 + +0.7
5,329,752 - +1.9
5,329,944 + -0.7
5,329,945 - +1.8
5,329,945 - +1.6
5,329,976 + -1.0
5,330,021 + Pf6N2E2_4604 0.13 +0.6
5,330,021 + Pf6N2E2_4604 0.13 -0.9
5,330,063 + Pf6N2E2_4604 0.22 +0.2
5,330,064 - Pf6N2E2_4604 0.23 -0.7
5,330,066 + Pf6N2E2_4604 0.23 -0.7
5,330,066 + Pf6N2E2_4604 0.23 +0.1
5,330,247 + Pf6N2E2_4604 0.65 -0.9
5,330,248 - Pf6N2E2_4604 0.66 +0.6
5,330,248 - Pf6N2E2_4604 0.66 +0.3
5,330,258 + Pf6N2E2_4604 0.68 -1.6
5,330,258 + Pf6N2E2_4604 0.68 -1.6
5,330,258 + Pf6N2E2_4604 0.68 -0.8
5,330,258 + Pf6N2E2_4604 0.68 -0.2
5,330,258 + Pf6N2E2_4604 0.68 -0.4
5,330,258 + Pf6N2E2_4604 0.68 -1.4
5,330,258 + Pf6N2E2_4604 0.68 -0.7
5,330,258 + Pf6N2E2_4604 0.68 -2.0
5,330,259 - Pf6N2E2_4604 0.68 -1.8
5,330,259 - Pf6N2E2_4604 0.68 -1.4
5,330,259 - Pf6N2E2_4604 0.68 -0.9
5,330,259 - Pf6N2E2_4604 0.68 +4.7
5,330,259 - Pf6N2E2_4604 0.68 -1.3
5,330,288 + Pf6N2E2_4604 0.75 -1.1
5,330,288 + Pf6N2E2_4604 0.75 +0.1
5,330,288 + Pf6N2E2_4604 0.75 -0.0
5,330,289 - Pf6N2E2_4604 0.75 -0.4
5,330,289 - Pf6N2E2_4604 0.75 -0.1
5,330,289 - Pf6N2E2_4604 0.75 +0.0
5,330,289 - Pf6N2E2_4604 0.75 -1.0
5,330,289 - Pf6N2E2_4604 0.75 -0.4
5,330,327 + Pf6N2E2_4604 0.84 +1.0
5,330,327 + Pf6N2E2_4604 0.84 -0.5
5,330,327 + Pf6N2E2_4604 0.84 -0.4
5,330,327 + Pf6N2E2_4604 0.84 +1.2
5,330,328 - Pf6N2E2_4604 0.84 +0.0
5,330,365 + -1.0
5,330,393 + -0.0
5,330,394 - -0.8

Or see this region's nucleotide sequence