Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3568 and Pf6N2E2_3569 are separated by 466 nucleotides Pf6N2E2_3569 and Pf6N2E2_3570 are separated by 224 nucleotides Pf6N2E2_3570 and Pf6N2E2_3571 overlap by 4 nucleotides
Pf6N2E2_3568 - Unsaturated fatty acid biosythesis repressor FabR, TetR family, at 4,159,912 to 4,160,544
_3568
Pf6N2E2_3569 - hypothetical protein, at 4,161,011 to 4,161,394
_3569
Pf6N2E2_3570 - Urease accessory protein UreE, at 4,161,619 to 4,162,119
_3570
Pf6N2E2_3571 - Urease accessory protein UreF, at 4,162,116 to 4,162,790
_3571
Position (kb)
4161
4162 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7
8
9
10 at 4160.057 kb on - strand, within Pf6N2E2_3568 at 4160.142 kb on - strand, within Pf6N2E2_3568 at 4160.172 kb on + strand, within Pf6N2E2_3568 at 4160.172 kb on + strand, within Pf6N2E2_3568 at 4160.553 kb on + strand at 4160.553 kb on + strand at 4160.553 kb on + strand at 4160.746 kb on + strand at 4160.746 kb on + strand at 4160.746 kb on + strand at 4160.746 kb on + strand at 4160.747 kb on - strand at 4160.747 kb on - strand at 4160.747 kb on - strand at 4160.747 kb on - strand at 4160.747 kb on - strand at 4160.747 kb on - strand at 4160.747 kb on - strand at 4160.822 kb on + strand at 4160.823 kb on - strand at 4160.823 kb on - strand at 4160.823 kb on - strand at 4160.823 kb on - strand at 4160.823 kb on - strand at 4160.823 kb on - strand at 4160.940 kb on + strand at 4160.940 kb on + strand at 4160.940 kb on + strand at 4160.940 kb on + strand at 4160.940 kb on + strand at 4160.940 kb on + strand at 4160.940 kb on + strand at 4160.940 kb on + strand at 4160.940 kb on + strand at 4160.940 kb on + strand at 4160.940 kb on + strand at 4160.941 kb on - strand at 4160.941 kb on - strand at 4160.941 kb on - strand at 4160.941 kb on - strand at 4160.941 kb on - strand at 4160.941 kb on - strand at 4160.941 kb on - strand at 4160.941 kb on - strand at 4160.941 kb on - strand at 4160.941 kb on - strand at 4160.941 kb on - strand at 4160.941 kb on - strand at 4160.944 kb on + strand at 4160.944 kb on + strand at 4160.944 kb on + strand at 4160.944 kb on + strand at 4160.945 kb on - strand at 4160.945 kb on - strand at 4160.945 kb on - strand at 4160.945 kb on - strand at 4160.945 kb on - strand at 4160.945 kb on - strand at 4161.010 kb on + strand at 4161.011 kb on - strand at 4161.113 kb on + strand at 4161.113 kb on + strand, within Pf6N2E2_3569 at 4161.116 kb on + strand, within Pf6N2E2_3569 at 4161.116 kb on + strand, within Pf6N2E2_3569 at 4161.545 kb on + strand at 4161.552 kb on + strand at 4161.553 kb on - strand at 4161.584 kb on + strand at 4161.585 kb on - strand at 4161.886 kb on + strand, within Pf6N2E2_3570 at 4161.952 kb on + strand, within Pf6N2E2_3570 at 4161.952 kb on + strand, within Pf6N2E2_3570 at 4161.952 kb on + strand, within Pf6N2E2_3570 at 4161.953 kb on - strand, within Pf6N2E2_3570 at 4161.953 kb on - strand, within Pf6N2E2_3570 at 4161.953 kb on - strand, within Pf6N2E2_3570 at 4161.953 kb on - strand, within Pf6N2E2_3570 at 4161.953 kb on - strand, within Pf6N2E2_3570 at 4162.033 kb on + strand, within Pf6N2E2_3570 at 4162.033 kb on + strand, within Pf6N2E2_3570 at 4162.081 kb on + strand at 4162.081 kb on + strand at 4162.081 kb on + strand at 4162.082 kb on - strand at 4162.082 kb on - strand at 4162.173 kb on + strand at 4162.179 kb on + strand at 4162.179 kb on + strand at 4162.179 kb on + strand at 4162.179 kb on + strand at 4162.179 kb on + strand at 4162.179 kb on + strand at 4162.180 kb on - strand at 4162.180 kb on - strand at 4162.180 kb on - strand at 4162.180 kb on - strand at 4162.180 kb on - strand at 4162.180 kb on - strand at 4162.180 kb on - strand at 4162.392 kb on + strand, within Pf6N2E2_3571
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 4,160,057 - Pf6N2E2_3568 0.23 +1.2 4,160,142 - Pf6N2E2_3568 0.36 -1.4 4,160,172 + Pf6N2E2_3568 0.41 +1.6 4,160,172 + Pf6N2E2_3568 0.41 +1.4 4,160,553 + -3.1 4,160,553 + -0.8 4,160,553 + +0.1 4,160,746 + +1.4 4,160,746 + +1.0 4,160,746 + +0.6 4,160,746 + +1.3 4,160,747 - +1.5 4,160,747 - -0.1 4,160,747 - +2.1 4,160,747 - +1.3 4,160,747 - -1.5 4,160,747 - +1.7 4,160,747 - +0.8 4,160,822 + -0.6 4,160,823 - -0.1 4,160,823 - +0.5 4,160,823 - +0.1 4,160,823 - +2.2 4,160,823 - +0.3 4,160,823 - +0.2 4,160,940 + +1.9 4,160,940 + +0.9 4,160,940 + +10.4 4,160,940 + -0.7 4,160,940 + +9.7 4,160,940 + -1.0 4,160,940 + +3.2 4,160,940 + +1.8 4,160,940 + +2.5 4,160,940 + -1.1 4,160,940 + +1.0 4,160,941 - -0.0 4,160,941 - +0.5 4,160,941 - -0.9 4,160,941 - -0.9 4,160,941 - +0.3 4,160,941 - +0.6 4,160,941 - -0.7 4,160,941 - -0.1 4,160,941 - +0.5 4,160,941 - +0.1 4,160,941 - +0.2 4,160,941 - +0.6 4,160,944 + +10.1 4,160,944 + +10.5 4,160,944 + +6.5 4,160,944 + +10.2 4,160,945 - +1.1 4,160,945 - +1.5 4,160,945 - +0.9 4,160,945 - +0.4 4,160,945 - +1.6 4,160,945 - +1.0 4,161,010 + -0.4 4,161,011 - -1.6 4,161,113 + +5.1 4,161,113 + Pf6N2E2_3569 0.27 +0.8 4,161,116 + Pf6N2E2_3569 0.27 -1.8 4,161,116 + Pf6N2E2_3569 0.27 -2.4 4,161,545 + +0.7 4,161,552 + +0.0 4,161,553 - +0.3 4,161,584 + +1.7 4,161,585 - +0.0 4,161,886 + Pf6N2E2_3570 0.53 -0.7 4,161,952 + Pf6N2E2_3570 0.66 +0.7 4,161,952 + Pf6N2E2_3570 0.66 +0.9 4,161,952 + Pf6N2E2_3570 0.66 -0.6 4,161,953 - Pf6N2E2_3570 0.67 +1.0 4,161,953 - Pf6N2E2_3570 0.67 -1.5 4,161,953 - Pf6N2E2_3570 0.67 +1.0 4,161,953 - Pf6N2E2_3570 0.67 +2.8 4,161,953 - Pf6N2E2_3570 0.67 +1.0 4,162,033 + Pf6N2E2_3570 0.83 -1.2 4,162,033 + Pf6N2E2_3570 0.83 +1.3 4,162,081 + -0.1 4,162,081 + -1.2 4,162,081 + +0.4 4,162,082 - +0.1 4,162,082 - +0.4 4,162,173 + +0.8 4,162,179 + -1.2 4,162,179 + -0.4 4,162,179 + -0.1 4,162,179 + -0.4 4,162,179 + -2.3 4,162,179 + +0.4 4,162,180 - -0.0 4,162,180 - +2.3 4,162,180 - +0.3 4,162,180 - -1.3 4,162,180 - +1.5 4,162,180 - +0.3 4,162,180 - -1.3 4,162,392 + Pf6N2E2_3571 0.41 +1.1
Or see this region's nucleotide sequence