Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1688 and Pf6N2E2_1689 are separated by 265 nucleotides Pf6N2E2_1689 and Pf6N2E2_1690 are separated by 33 nucleotides
Pf6N2E2_1688 - Permeases of the major facilitator superfamily, at 1,997,325 to 1,998,581
_1688
Pf6N2E2_1689 - Probable porin, at 1,998,847 to 1,999,323
_1689
Pf6N2E2_1690 - Mobile element protein, at 1,999,357 to 2,000,526
_1690
Position (kb)
1998
1999
2000 Strain fitness (log2 ratio)
-2
-1
0
1 at 1997.928 kb on - strand, within Pf6N2E2_1688 at 1997.928 kb on - strand, within Pf6N2E2_1688 at 1998.080 kb on - strand, within Pf6N2E2_1688 at 1998.186 kb on - strand, within Pf6N2E2_1688 at 1998.494 kb on - strand at 1998.494 kb on - strand at 1998.557 kb on + strand at 1998.557 kb on + strand at 1998.558 kb on - strand at 1998.831 kb on - strand at 1998.831 kb on - strand at 1998.831 kb on - strand at 1998.848 kb on + strand at 1998.848 kb on + strand at 1998.849 kb on - strand at 1998.849 kb on - strand at 1998.880 kb on - strand at 1998.880 kb on - strand at 1998.923 kb on + strand, within Pf6N2E2_1689 at 1998.923 kb on + strand, within Pf6N2E2_1689 at 1998.923 kb on + strand, within Pf6N2E2_1689 at 1998.924 kb on - strand, within Pf6N2E2_1689 at 1998.924 kb on - strand, within Pf6N2E2_1689 at 1998.924 kb on - strand, within Pf6N2E2_1689 at 1998.924 kb on - strand, within Pf6N2E2_1689 at 1998.924 kb on - strand, within Pf6N2E2_1689 at 1998.958 kb on - strand, within Pf6N2E2_1689 at 1998.958 kb on - strand, within Pf6N2E2_1689 at 1998.958 kb on - strand, within Pf6N2E2_1689 at 1998.974 kb on + strand, within Pf6N2E2_1689 at 1998.975 kb on - strand, within Pf6N2E2_1689 at 1999.091 kb on + strand, within Pf6N2E2_1689 at 1999.163 kb on + strand, within Pf6N2E2_1689 at 1999.184 kb on + strand, within Pf6N2E2_1689 at 1999.184 kb on + strand, within Pf6N2E2_1689 at 1999.184 kb on + strand, within Pf6N2E2_1689 at 1999.184 kb on + strand, within Pf6N2E2_1689 at 1999.184 kb on + strand, within Pf6N2E2_1689 at 1999.185 kb on - strand, within Pf6N2E2_1689 at 1999.185 kb on - strand, within Pf6N2E2_1689 at 1999.185 kb on - strand, within Pf6N2E2_1689 at 1999.338 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,997,928 - Pf6N2E2_1688 0.48 -0.9 1,997,928 - Pf6N2E2_1688 0.48 -1.9 1,998,080 - Pf6N2E2_1688 0.60 +0.7 1,998,186 - Pf6N2E2_1688 0.68 -0.2 1,998,494 - -0.3 1,998,494 - -0.7 1,998,557 + -0.1 1,998,557 + -0.0 1,998,558 - -1.2 1,998,831 - -1.8 1,998,831 - -2.1 1,998,831 - -0.7 1,998,848 + +1.5 1,998,848 + -0.3 1,998,849 - -0.4 1,998,849 - +0.0 1,998,880 - +0.6 1,998,880 - +0.1 1,998,923 + Pf6N2E2_1689 0.16 -0.7 1,998,923 + Pf6N2E2_1689 0.16 +0.3 1,998,923 + Pf6N2E2_1689 0.16 -0.1 1,998,924 - Pf6N2E2_1689 0.16 -0.8 1,998,924 - Pf6N2E2_1689 0.16 -1.8 1,998,924 - Pf6N2E2_1689 0.16 +1.7 1,998,924 - Pf6N2E2_1689 0.16 -0.5 1,998,924 - Pf6N2E2_1689 0.16 -1.3 1,998,958 - Pf6N2E2_1689 0.23 +0.2 1,998,958 - Pf6N2E2_1689 0.23 -1.4 1,998,958 - Pf6N2E2_1689 0.23 -0.0 1,998,974 + Pf6N2E2_1689 0.27 +1.7 1,998,975 - Pf6N2E2_1689 0.27 +0.1 1,999,091 + Pf6N2E2_1689 0.51 -0.6 1,999,163 + Pf6N2E2_1689 0.66 -2.5 1,999,184 + Pf6N2E2_1689 0.71 -1.5 1,999,184 + Pf6N2E2_1689 0.71 -1.6 1,999,184 + Pf6N2E2_1689 0.71 -0.2 1,999,184 + Pf6N2E2_1689 0.71 +0.4 1,999,184 + Pf6N2E2_1689 0.71 -0.5 1,999,185 - Pf6N2E2_1689 0.71 -0.3 1,999,185 - Pf6N2E2_1689 0.71 -0.5 1,999,185 - Pf6N2E2_1689 0.71 -0.3 1,999,338 - -0.5
Or see this region's nucleotide sequence