Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1733 and Shew_1734 are separated by 30 nucleotides Shew_1734 and Shew_1735 are separated by 321 nucleotides Shew_1735 and Shew_1736 are separated by 64 nucleotides Shew_1736 and Shew_1737 are separated by 43 nucleotides
Shew_1733: Shew_1733 - thioesterase superfamily protein (RefSeq), at 1,984,164 to 1,984,628
_1733
Shew_1734: Shew_1734 - hypothetical protein (RefSeq), at 1,984,659 to 1,985,048
_1734
Shew_1735: Shew_1735 - TonB-dependent receptor (RefSeq), at 1,985,370 to 1,987,511
_1735
Shew_1736: Shew_1736 - hypothetical protein (RefSeq), at 1,987,576 to 1,987,836
_1736
Shew_1737: Shew_1737 - nicotinamide mononucleotide transporter PnuC (RefSeq), at 1,987,880 to 1,988,527
_1737
Position (kb)
1985
1986
1987
1988 Strain fitness (log2 ratio)
-2
-1
0
1 at 1984.449 kb on - strand, within Shew_1733 at 1984.945 kb on + strand, within Shew_1734 at 1984.945 kb on + strand, within Shew_1734 at 1985.822 kb on + strand, within Shew_1735 at 1986.335 kb on + strand, within Shew_1735 at 1986.523 kb on - strand, within Shew_1735 at 1986.770 kb on - strand, within Shew_1735 at 1986.865 kb on + strand, within Shew_1735 at 1986.865 kb on + strand, within Shew_1735 at 1987.312 kb on - strand at 1987.653 kb on + strand, within Shew_1736 at 1987.984 kb on - strand, within Shew_1737 at 1988.424 kb on + strand, within Shew_1737 at 1988.424 kb on + strand, within Shew_1737 at 1988.432 kb on - strand, within Shew_1737 at 1988.432 kb on - strand, within Shew_1737 at 1988.463 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,984,449 - Shew_1733 0.61 +0.6 1,984,945 + Shew_1734 0.73 +0.1 1,984,945 + Shew_1734 0.73 +0.4 1,985,822 + Shew_1735 0.21 +0.1 1,986,335 + Shew_1735 0.45 -0.8 1,986,523 - Shew_1735 0.54 -0.4 1,986,770 - Shew_1735 0.65 -1.8 1,986,865 + Shew_1735 0.70 -0.6 1,986,865 + Shew_1735 0.70 -0.1 1,987,312 - +0.2 1,987,653 + Shew_1736 0.30 -0.5 1,987,984 - Shew_1737 0.16 -1.6 1,988,424 + Shew_1737 0.84 -0.2 1,988,424 + Shew_1737 0.84 -0.0 1,988,432 - Shew_1737 0.85 +0.1 1,988,432 - Shew_1737 0.85 -0.8 1,988,463 + -0.3
Or see this region's nucleotide sequence