Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3246

Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3245 and GFF3246 are separated by 68 nucleotidesGFF3246 and GFF3247 are separated by 185 nucleotidesGFF3247 and GFF3248 are separated by 71 nucleotides GFF3245 - 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, FabA form (EC 4.2.1.59), at 19,774 to 20,292 GFF3245 GFF3246 - ATP-dependent protease La (EC 3.4.21.53) Type II, at 20,361 to 22,121 GFF3246 GFF3247 - Macrodomain Ter protein YcbG, at 22,307 to 22,759 GFF3247 GFF3248 - Outer membrane protein A precursor, at 22,831 to 23,907 GFF3248 Position (kb) 20 21 22 23Strain fitness (log2 ratio) -2 -1 0 1 2at 19.368 kb on + strandat 19.430 kb on + strandat 19.490 kb on + strandat 19.555 kb on + strandat 19.761 kb on - strandat 20.331 kb on - strandat 20.372 kb on + strandat 20.791 kb on - strand, within GFF3246at 20.791 kb on - strand, within GFF3246at 20.848 kb on - strand, within GFF3246at 20.925 kb on - strand, within GFF3246at 21.288 kb on - strand, within GFF3246at 21.393 kb on - strand, within GFF3246at 21.393 kb on - strand, within GFF3246at 21.830 kb on + strand, within GFF3246at 21.938 kb on + strand, within GFF3246at 21.972 kb on + strandat 21.973 kb on - strandat 22.020 kb on + strandat 22.021 kb on - strandat 22.060 kb on - strandat 22.069 kb on + strandat 22.145 kb on + strandat 22.166 kb on - strandat 22.397 kb on + strand, within GFF3247at 22.489 kb on + strand, within GFF3247at 23.067 kb on - strand, within GFF3248

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI
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19,368 + -0.5
19,430 + -0.0
19,490 + -0.0
19,555 + -0.0
19,761 - -0.4
20,331 - -0.1
20,372 + -1.0
20,791 - GFF3246 0.24 -0.3
20,791 - GFF3246 0.24 -0.7
20,848 - GFF3246 0.28 +0.7
20,925 - GFF3246 0.32 -0.9
21,288 - GFF3246 0.53 +0.6
21,393 - GFF3246 0.59 -0.8
21,393 - GFF3246 0.59 -1.0
21,830 + GFF3246 0.83 -1.9
21,938 + GFF3246 0.90 -0.7
21,972 + -2.1
21,973 - -0.1
22,020 + -0.8
22,021 - +0.3
22,060 - -1.1
22,069 + -0.4
22,145 + +0.6
22,166 - -0.8
22,397 + GFF3247 0.20 +1.3
22,489 + GFF3247 0.40 +0.1
23,067 - GFF3248 0.22 +2.1

Or see this region's nucleotide sequence