Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2831 and GFF2832 are separated by 52 nucleotides GFF2832 and GFF2833 are separated by 12 nucleotides GFF2833 and GFF2834 are separated by 57 nucleotides
GFF2831 - PTS system, mannose-specific IIA component (EC 2.7.1.69) / PTS system, mannose-specific IIB component (EC 2.7.1.69), at 353,805 to 354,773
GFF2831
GFF2832 - PTS system, mannose-specific IIC component (EC 2.7.1.69), at 354,826 to 355,626
GFF2832
GFF2833 - PTS system, mannose-specific IID component (EC 2.7.1.69), at 355,639 to 356,490
GFF2833
GFF2834 - FIG00510464: hypothetical protein, at 356,548 to 357,018
GFF2834
Position (kb) 354
355
356 Strain fitness (log2 ratio) -2
-1
0
1 at 353.966 kb on + strand, within GFF2831 at 353.981 kb on + strand, within GFF2831 at 354.005 kb on + strand, within GFF2831 at 354.006 kb on - strand, within GFF2831 at 354.372 kb on - strand, within GFF2831 at 354.372 kb on - strand, within GFF2831 at 354.542 kb on + strand, within GFF2831 at 354.802 kb on + strand at 354.803 kb on - strand at 354.807 kb on + strand at 354.813 kb on + strand at 354.813 kb on + strand at 354.813 kb on + strand at 354.834 kb on + strand at 354.939 kb on - strand, within GFF2832 at 355.168 kb on - strand, within GFF2832 at 355.406 kb on - strand, within GFF2832 at 355.537 kb on + strand, within GFF2832 at 355.747 kb on - strand, within GFF2833 at 355.867 kb on + strand, within GFF2833 at 356.391 kb on + strand, within GFF2833 at 356.416 kb on - strand at 356.500 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 353,966 + GFF2831 0.17 -0.4 353,981 + GFF2831 0.18 +0.2 354,005 + GFF2831 0.21 -1.9 354,006 - GFF2831 0.21 +1.1 354,372 - GFF2831 0.59 -0.1 354,372 - GFF2831 0.59 +0.4 354,542 + GFF2831 0.76 -0.1 354,802 + -0.2 354,803 - -0.2 354,807 + +0.6 354,813 + -1.9 354,813 + +0.4 354,813 + -0.3 354,834 + -1.1 354,939 - GFF2832 0.14 +0.4 355,168 - GFF2832 0.43 -0.1 355,406 - GFF2832 0.72 -2.1 355,537 + GFF2832 0.89 -2.1 355,747 - GFF2833 0.13 -0.4 355,867 + GFF2833 0.27 -0.3 356,391 + GFF2833 0.88 +1.6 356,416 - +0.1 356,500 - +0.2
Or see this region's nucleotide sequence