Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2355

Experiment: copper (II) chloride 3 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2352 and Pf6N2E2_2353 overlap by 4 nucleotidesPf6N2E2_2353 and Pf6N2E2_2354 are separated by 59 nucleotidesPf6N2E2_2354 and Pf6N2E2_2355 are separated by 3 nucleotidesPf6N2E2_2355 and Pf6N2E2_2356 are separated by 158 nucleotidesPf6N2E2_2356 and Pf6N2E2_2357 are separated by 32 nucleotides Pf6N2E2_2352 - Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ), at 2,787,229 to 2,787,948 _2352 Pf6N2E2_2353 - cell invasion protein - cytoplasmic, at 2,787,945 to 2,788,253 _2353 Pf6N2E2_2354 - MxiH protein; Type III secretion cytoplasmic protein (YscF), at 2,788,313 to 2,788,576 _2354 Pf6N2E2_2355 - MxiG protein; Pathogenicity 1 island effector protein, at 2,788,580 to 2,789,719 _2355 Pf6N2E2_2356 - Hpa2 protein, at 2,789,878 to 2,790,315 _2356 Pf6N2E2_2357 - Invasion protein iagA, at 2,790,348 to 2,791,409 _2357 Position (kb) 2788 2789 2790Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2787.635 kb on + strand, within Pf6N2E2_2352at 2787.636 kb on - strand, within Pf6N2E2_2352at 2788.024 kb on + strand, within Pf6N2E2_2353at 2788.024 kb on + strand, within Pf6N2E2_2353at 2788.024 kb on + strand, within Pf6N2E2_2353at 2788.025 kb on - strand, within Pf6N2E2_2353at 2788.025 kb on - strand, within Pf6N2E2_2353at 2788.056 kb on + strand, within Pf6N2E2_2353at 2788.056 kb on + strand, within Pf6N2E2_2353at 2788.056 kb on + strand, within Pf6N2E2_2353at 2788.056 kb on + strand, within Pf6N2E2_2353at 2788.057 kb on - strand, within Pf6N2E2_2353at 2788.057 kb on - strand, within Pf6N2E2_2353at 2788.253 kb on + strandat 2788.253 kb on + strandat 2788.253 kb on + strandat 2788.253 kb on + strandat 2788.253 kb on + strandat 2788.253 kb on + strandat 2788.253 kb on + strandat 2788.254 kb on - strandat 2788.254 kb on - strandat 2788.254 kb on - strandat 2788.388 kb on + strand, within Pf6N2E2_2354at 2788.388 kb on + strand, within Pf6N2E2_2354at 2788.388 kb on + strand, within Pf6N2E2_2354at 2788.388 kb on + strand, within Pf6N2E2_2354at 2788.395 kb on + strand, within Pf6N2E2_2354at 2788.395 kb on + strand, within Pf6N2E2_2354at 2788.396 kb on - strand, within Pf6N2E2_2354at 2788.417 kb on - strand, within Pf6N2E2_2354at 2788.568 kb on - strandat 2788.576 kb on + strandat 2788.576 kb on + strandat 2788.576 kb on + strandat 2788.576 kb on + strandat 2788.576 kb on + strandat 2788.576 kb on + strandat 2788.576 kb on + strandat 2788.577 kb on - strandat 2788.577 kb on - strandat 2788.577 kb on - strandat 2788.577 kb on - strandat 2788.577 kb on - strandat 2788.577 kb on - strandat 2788.577 kb on - strandat 2788.577 kb on - strandat 2788.586 kb on - strandat 2788.586 kb on - strandat 2788.586 kb on - strandat 2788.632 kb on + strandat 2788.632 kb on + strandat 2788.633 kb on - strandat 2788.633 kb on - strandat 2788.704 kb on + strand, within Pf6N2E2_2355at 2788.704 kb on + strand, within Pf6N2E2_2355at 2788.705 kb on - strand, within Pf6N2E2_2355at 2788.740 kb on + strand, within Pf6N2E2_2355at 2788.741 kb on - strand, within Pf6N2E2_2355at 2788.741 kb on - strand, within Pf6N2E2_2355at 2789.178 kb on + strand, within Pf6N2E2_2355at 2789.178 kb on + strand, within Pf6N2E2_2355at 2789.179 kb on - strand, within Pf6N2E2_2355at 2789.179 kb on - strand, within Pf6N2E2_2355at 2789.486 kb on + strand, within Pf6N2E2_2355at 2789.712 kb on - strandat 2789.732 kb on + strandat 2789.733 kb on - strandat 2789.764 kb on + strandat 2789.796 kb on + strandat 2789.797 kb on - strandat 2789.797 kb on - strandat 2789.939 kb on + strand, within Pf6N2E2_2356at 2789.984 kb on + strand, within Pf6N2E2_2356at 2790.483 kb on + strand, within Pf6N2E2_2357at 2790.508 kb on + strand, within Pf6N2E2_2357at 2790.509 kb on - strand, within Pf6N2E2_2357

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Per-strain Table

Position Strand Gene LocusTag Fraction copper (II) chloride 3 mM
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2,787,635 + Pf6N2E2_2352 0.56 -3.2
2,787,636 - Pf6N2E2_2352 0.57 -1.3
2,788,024 + Pf6N2E2_2353 0.26 +0.1
2,788,024 + Pf6N2E2_2353 0.26 +0.6
2,788,024 + Pf6N2E2_2353 0.26 -0.9
2,788,025 - Pf6N2E2_2353 0.26 -1.1
2,788,025 - Pf6N2E2_2353 0.26 -0.1
2,788,056 + Pf6N2E2_2353 0.36 -0.6
2,788,056 + Pf6N2E2_2353 0.36 -0.0
2,788,056 + Pf6N2E2_2353 0.36 +1.2
2,788,056 + Pf6N2E2_2353 0.36 -0.3
2,788,057 - Pf6N2E2_2353 0.36 -1.8
2,788,057 - Pf6N2E2_2353 0.36 +0.2
2,788,253 + +0.4
2,788,253 + +0.6
2,788,253 + -0.5
2,788,253 + +1.3
2,788,253 + +0.5
2,788,253 + -1.8
2,788,253 + +0.9
2,788,254 - +0.1
2,788,254 - +1.1
2,788,254 - +0.7
2,788,388 + Pf6N2E2_2354 0.28 -1.5
2,788,388 + Pf6N2E2_2354 0.28 -0.6
2,788,388 + Pf6N2E2_2354 0.28 +0.7
2,788,388 + Pf6N2E2_2354 0.28 -0.7
2,788,395 + Pf6N2E2_2354 0.31 -0.6
2,788,395 + Pf6N2E2_2354 0.31 +0.2
2,788,396 - Pf6N2E2_2354 0.31 -0.5
2,788,417 - Pf6N2E2_2354 0.39 -1.1
2,788,568 - -3.0
2,788,576 + -0.6
2,788,576 + -0.0
2,788,576 + +1.8
2,788,576 + +0.5
2,788,576 + +0.5
2,788,576 + +1.4
2,788,576 + -1.4
2,788,577 - -0.1
2,788,577 - +0.4
2,788,577 - +0.2
2,788,577 - +0.8
2,788,577 - -0.9
2,788,577 - -0.5
2,788,577 - -0.1
2,788,577 - +0.1
2,788,586 - -0.2
2,788,586 - +0.9
2,788,586 - -0.5
2,788,632 + +0.7
2,788,632 + +0.3
2,788,633 - +1.1
2,788,633 - +0.7
2,788,704 + Pf6N2E2_2355 0.11 -0.0
2,788,704 + Pf6N2E2_2355 0.11 -3.6
2,788,705 - Pf6N2E2_2355 0.11 -1.2
2,788,740 + Pf6N2E2_2355 0.14 +0.0
2,788,741 - Pf6N2E2_2355 0.14 -0.0
2,788,741 - Pf6N2E2_2355 0.14 -1.6
2,789,178 + Pf6N2E2_2355 0.52 -1.0
2,789,178 + Pf6N2E2_2355 0.52 -0.3
2,789,179 - Pf6N2E2_2355 0.53 +0.6
2,789,179 - Pf6N2E2_2355 0.53 +0.2
2,789,486 + Pf6N2E2_2355 0.79 -2.0
2,789,712 - +0.6
2,789,732 + +0.4
2,789,733 - -0.6
2,789,764 + +0.1
2,789,796 + +0.8
2,789,797 - -0.2
2,789,797 - +0.4
2,789,939 + Pf6N2E2_2356 0.14 +1.7
2,789,984 + Pf6N2E2_2356 0.24 +0.4
2,790,483 + Pf6N2E2_2357 0.13 +0.2
2,790,508 + Pf6N2E2_2357 0.15 +0.4
2,790,509 - Pf6N2E2_2357 0.15 +0.3

Or see this region's nucleotide sequence