Experiment: copper (II) chloride 3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2352 and Pf6N2E2_2353 overlap by 4 nucleotides Pf6N2E2_2353 and Pf6N2E2_2354 are separated by 59 nucleotides Pf6N2E2_2354 and Pf6N2E2_2355 are separated by 3 nucleotides Pf6N2E2_2355 and Pf6N2E2_2356 are separated by 158 nucleotides Pf6N2E2_2356 and Pf6N2E2_2357 are separated by 32 nucleotides
Pf6N2E2_2352 - Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ), at 2,787,229 to 2,787,948
_2352
Pf6N2E2_2353 - cell invasion protein - cytoplasmic, at 2,787,945 to 2,788,253
_2353
Pf6N2E2_2354 - MxiH protein; Type III secretion cytoplasmic protein (YscF), at 2,788,313 to 2,788,576
_2354
Pf6N2E2_2355 - MxiG protein; Pathogenicity 1 island effector protein, at 2,788,580 to 2,789,719
_2355
Pf6N2E2_2356 - Hpa2 protein, at 2,789,878 to 2,790,315
_2356
Pf6N2E2_2357 - Invasion protein iagA, at 2,790,348 to 2,791,409
_2357
Position (kb)
2788
2789
2790 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2787.635 kb on + strand, within Pf6N2E2_2352 at 2787.636 kb on - strand, within Pf6N2E2_2352 at 2788.024 kb on + strand, within Pf6N2E2_2353 at 2788.024 kb on + strand, within Pf6N2E2_2353 at 2788.024 kb on + strand, within Pf6N2E2_2353 at 2788.025 kb on - strand, within Pf6N2E2_2353 at 2788.025 kb on - strand, within Pf6N2E2_2353 at 2788.056 kb on + strand, within Pf6N2E2_2353 at 2788.056 kb on + strand, within Pf6N2E2_2353 at 2788.056 kb on + strand, within Pf6N2E2_2353 at 2788.056 kb on + strand, within Pf6N2E2_2353 at 2788.057 kb on - strand, within Pf6N2E2_2353 at 2788.057 kb on - strand, within Pf6N2E2_2353 at 2788.253 kb on + strand at 2788.253 kb on + strand at 2788.253 kb on + strand at 2788.253 kb on + strand at 2788.253 kb on + strand at 2788.253 kb on + strand at 2788.253 kb on + strand at 2788.254 kb on - strand at 2788.254 kb on - strand at 2788.254 kb on - strand at 2788.388 kb on + strand, within Pf6N2E2_2354 at 2788.388 kb on + strand, within Pf6N2E2_2354 at 2788.388 kb on + strand, within Pf6N2E2_2354 at 2788.388 kb on + strand, within Pf6N2E2_2354 at 2788.395 kb on + strand, within Pf6N2E2_2354 at 2788.395 kb on + strand, within Pf6N2E2_2354 at 2788.396 kb on - strand, within Pf6N2E2_2354 at 2788.417 kb on - strand, within Pf6N2E2_2354 at 2788.568 kb on - strand at 2788.576 kb on + strand at 2788.576 kb on + strand at 2788.576 kb on + strand at 2788.576 kb on + strand at 2788.576 kb on + strand at 2788.576 kb on + strand at 2788.576 kb on + strand at 2788.577 kb on - strand at 2788.577 kb on - strand at 2788.577 kb on - strand at 2788.577 kb on - strand at 2788.577 kb on - strand at 2788.577 kb on - strand at 2788.577 kb on - strand at 2788.577 kb on - strand at 2788.586 kb on - strand at 2788.586 kb on - strand at 2788.586 kb on - strand at 2788.632 kb on + strand at 2788.632 kb on + strand at 2788.633 kb on - strand at 2788.633 kb on - strand at 2788.704 kb on + strand, within Pf6N2E2_2355 at 2788.704 kb on + strand, within Pf6N2E2_2355 at 2788.705 kb on - strand, within Pf6N2E2_2355 at 2788.740 kb on + strand, within Pf6N2E2_2355 at 2788.741 kb on - strand, within Pf6N2E2_2355 at 2788.741 kb on - strand, within Pf6N2E2_2355 at 2789.178 kb on + strand, within Pf6N2E2_2355 at 2789.178 kb on + strand, within Pf6N2E2_2355 at 2789.179 kb on - strand, within Pf6N2E2_2355 at 2789.179 kb on - strand, within Pf6N2E2_2355 at 2789.486 kb on + strand, within Pf6N2E2_2355 at 2789.712 kb on - strand at 2789.732 kb on + strand at 2789.733 kb on - strand at 2789.764 kb on + strand at 2789.796 kb on + strand at 2789.797 kb on - strand at 2789.797 kb on - strand at 2789.939 kb on + strand, within Pf6N2E2_2356 at 2789.984 kb on + strand, within Pf6N2E2_2356 at 2790.483 kb on + strand, within Pf6N2E2_2357 at 2790.508 kb on + strand, within Pf6N2E2_2357 at 2790.509 kb on - strand, within Pf6N2E2_2357
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 3 mM remove 2,787,635 + Pf6N2E2_2352 0.56 -3.2 2,787,636 - Pf6N2E2_2352 0.57 -1.3 2,788,024 + Pf6N2E2_2353 0.26 +0.1 2,788,024 + Pf6N2E2_2353 0.26 +0.6 2,788,024 + Pf6N2E2_2353 0.26 -0.9 2,788,025 - Pf6N2E2_2353 0.26 -1.1 2,788,025 - Pf6N2E2_2353 0.26 -0.1 2,788,056 + Pf6N2E2_2353 0.36 -0.6 2,788,056 + Pf6N2E2_2353 0.36 -0.0 2,788,056 + Pf6N2E2_2353 0.36 +1.2 2,788,056 + Pf6N2E2_2353 0.36 -0.3 2,788,057 - Pf6N2E2_2353 0.36 -1.8 2,788,057 - Pf6N2E2_2353 0.36 +0.2 2,788,253 + +0.4 2,788,253 + +0.6 2,788,253 + -0.5 2,788,253 + +1.3 2,788,253 + +0.5 2,788,253 + -1.8 2,788,253 + +0.9 2,788,254 - +0.1 2,788,254 - +1.1 2,788,254 - +0.7 2,788,388 + Pf6N2E2_2354 0.28 -1.5 2,788,388 + Pf6N2E2_2354 0.28 -0.6 2,788,388 + Pf6N2E2_2354 0.28 +0.7 2,788,388 + Pf6N2E2_2354 0.28 -0.7 2,788,395 + Pf6N2E2_2354 0.31 -0.6 2,788,395 + Pf6N2E2_2354 0.31 +0.2 2,788,396 - Pf6N2E2_2354 0.31 -0.5 2,788,417 - Pf6N2E2_2354 0.39 -1.1 2,788,568 - -3.0 2,788,576 + -0.6 2,788,576 + -0.0 2,788,576 + +1.8 2,788,576 + +0.5 2,788,576 + +0.5 2,788,576 + +1.4 2,788,576 + -1.4 2,788,577 - -0.1 2,788,577 - +0.4 2,788,577 - +0.2 2,788,577 - +0.8 2,788,577 - -0.9 2,788,577 - -0.5 2,788,577 - -0.1 2,788,577 - +0.1 2,788,586 - -0.2 2,788,586 - +0.9 2,788,586 - -0.5 2,788,632 + +0.7 2,788,632 + +0.3 2,788,633 - +1.1 2,788,633 - +0.7 2,788,704 + Pf6N2E2_2355 0.11 -0.0 2,788,704 + Pf6N2E2_2355 0.11 -3.6 2,788,705 - Pf6N2E2_2355 0.11 -1.2 2,788,740 + Pf6N2E2_2355 0.14 +0.0 2,788,741 - Pf6N2E2_2355 0.14 -0.0 2,788,741 - Pf6N2E2_2355 0.14 -1.6 2,789,178 + Pf6N2E2_2355 0.52 -1.0 2,789,178 + Pf6N2E2_2355 0.52 -0.3 2,789,179 - Pf6N2E2_2355 0.53 +0.6 2,789,179 - Pf6N2E2_2355 0.53 +0.2 2,789,486 + Pf6N2E2_2355 0.79 -2.0 2,789,712 - +0.6 2,789,732 + +0.4 2,789,733 - -0.6 2,789,764 + +0.1 2,789,796 + +0.8 2,789,797 - -0.2 2,789,797 - +0.4 2,789,939 + Pf6N2E2_2356 0.14 +1.7 2,789,984 + Pf6N2E2_2356 0.24 +0.4 2,790,483 + Pf6N2E2_2357 0.13 +0.2 2,790,508 + Pf6N2E2_2357 0.15 +0.4 2,790,509 - Pf6N2E2_2357 0.15 +0.3
Or see this region's nucleotide sequence