Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF930 and GFF931 are separated by 226 nucleotides GFF931 and GFF932 overlap by 4 nucleotides GFF932 and GFF933 are separated by 73 nucleotides GFF933 and GFF934 are separated by 55 nucleotides
GFF930 - Aspartate carbamoyltransferase (EC 2.1.3.2), at 107,322 to 108,257
GFF930
GFF931 - Arginine pathway regulatory protein ArgR, repressor of arg regulon, at 108,484 to 108,972
GFF931
GFF932 - hypothetical protein, at 108,969 to 109,085
GFF932
GFF933 - Arginine/ornithine antiporter ArcD, at 109,159 to 110,562
GFF933
GFF934 - Ornithine carbamoyltransferase (EC 2.1.3.3), at 110,618 to 111,622
GFF934
Position (kb)
109
110
111 Strain fitness (log2 ratio)
-2
-1
0
1 at 108.194 kb on + strand at 108.230 kb on + strand at 108.261 kb on + strand at 108.573 kb on + strand, within GFF931 at 108.573 kb on + strand, within GFF931 at 108.685 kb on - strand, within GFF931 at 108.685 kb on - strand, within GFF931 at 108.953 kb on + strand at 108.954 kb on - strand at 108.992 kb on + strand, within GFF932 at 109.119 kb on + strand at 109.135 kb on + strand at 109.161 kb on - strand at 109.161 kb on - strand at 109.239 kb on - strand at 109.248 kb on - strand at 109.863 kb on + strand, within GFF933 at 109.924 kb on + strand, within GFF933 at 109.924 kb on + strand, within GFF933 at 110.154 kb on - strand, within GFF933 at 110.154 kb on - strand, within GFF933 at 110.154 kb on - strand, within GFF933 at 110.158 kb on - strand, within GFF933 at 110.158 kb on - strand, within GFF933 at 110.333 kb on + strand, within GFF933 at 110.348 kb on + strand, within GFF933 at 110.348 kb on + strand, within GFF933 at 110.414 kb on + strand, within GFF933 at 110.515 kb on - strand at 110.533 kb on - strand at 110.533 kb on - strand at 110.607 kb on - strand at 110.930 kb on - strand, within GFF934 at 111.138 kb on + strand, within GFF934 at 111.319 kb on - strand, within GFF934 at 111.393 kb on + strand, within GFF934 at 111.393 kb on + strand, within GFF934
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 108,194 + +0.1 108,230 + +0.8 108,261 + -0.1 108,573 + GFF931 0.18 +0.2 108,573 + GFF931 0.18 +0.1 108,685 - GFF931 0.41 -0.1 108,685 - GFF931 0.41 -0.0 108,953 + -0.4 108,954 - -0.2 108,992 + GFF932 0.20 +0.0 109,119 + +0.3 109,135 + -0.1 109,161 - -0.6 109,161 - +1.3 109,239 - -0.6 109,248 - -0.7 109,863 + GFF933 0.50 +0.4 109,924 + GFF933 0.54 -1.2 109,924 + GFF933 0.54 +0.2 110,154 - GFF933 0.71 -0.4 110,154 - GFF933 0.71 -0.1 110,154 - GFF933 0.71 -0.0 110,158 - GFF933 0.71 +0.5 110,158 - GFF933 0.71 +0.7 110,333 + GFF933 0.84 +0.6 110,348 + GFF933 0.85 +0.2 110,348 + GFF933 0.85 -0.2 110,414 + GFF933 0.89 -0.8 110,515 - -0.1 110,533 - -2.0 110,533 - +0.0 110,607 - +0.0 110,930 - GFF934 0.31 -0.0 111,138 + GFF934 0.52 -0.6 111,319 - GFF934 0.70 -0.6 111,393 + GFF934 0.77 +0.7 111,393 + GFF934 0.77 -0.4
Or see this region's nucleotide sequence