Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3035 and GFF3036 are separated by 810 nucleotides GFF3036 and GFF3037 overlap by 19 nucleotides GFF3037 and GFF3038 are separated by 216 nucleotides
GFF3035 - D-mannonate oxidoreductase (EC 1.1.1.57), at 49,500 to 50,972
GFF3035
GFF3036 - Muconolactone isomerase (EC 5.3.3.4),putative, at 51,783 to 51,917
GFF3036
GFF3037 - hypothetical protein, at 51,899 to 52,015
GFF3037
GFF3038 - Hexuronate utilization operon transcriptional repressor ExuR, at 52,232 to 52,981
GFF3038
Position (kb)
51
52
53 Strain fitness (log2 ratio)
-1
0
1 at 51.651 kb on + strand at 51.651 kb on + strand at 51.652 kb on - strand at 51.652 kb on - strand at 51.652 kb on - strand at 51.749 kb on + strand at 51.754 kb on - strand at 51.774 kb on - strand at 51.781 kb on - strand at 51.940 kb on + strand, within GFF3037 at 51.955 kb on - strand, within GFF3037 at 52.046 kb on + strand at 52.047 kb on - strand at 52.100 kb on + strand at 52.101 kb on - strand at 52.113 kb on + strand at 52.114 kb on - strand at 52.381 kb on + strand, within GFF3038 at 52.382 kb on - strand, within GFF3038 at 52.429 kb on - strand, within GFF3038 at 52.607 kb on - strand, within GFF3038 at 52.609 kb on + strand, within GFF3038 at 52.609 kb on + strand, within GFF3038 at 52.610 kb on - strand, within GFF3038 at 52.624 kb on + strand, within GFF3038 at 52.624 kb on + strand, within GFF3038 at 52.670 kb on - strand, within GFF3038 at 52.722 kb on + strand, within GFF3038 at 52.771 kb on - strand, within GFF3038 at 52.820 kb on - strand, within GFF3038 at 52.944 kb on - strand at 52.987 kb on + strand at 52.987 kb on + strand at 52.987 kb on + strand at 52.991 kb on + strand at 52.991 kb on + strand at 52.992 kb on - strand at 52.992 kb on - strand at 52.992 kb on - strand at 52.992 kb on - strand at 52.995 kb on + strand at 52.996 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 51,651 + -0.2 51,651 + -0.1 51,652 - -0.3 51,652 - +0.7 51,652 - +0.3 51,749 + -0.1 51,754 - -0.3 51,774 - +0.7 51,781 - +0.1 51,940 + GFF3037 0.35 -0.5 51,955 - GFF3037 0.48 -0.7 52,046 + +0.3 52,047 - -0.6 52,100 + -0.1 52,101 - +0.2 52,113 + -1.2 52,114 - +0.3 52,381 + GFF3038 0.20 +0.1 52,382 - GFF3038 0.20 +0.3 52,429 - GFF3038 0.26 +1.0 52,607 - GFF3038 0.50 -1.4 52,609 + GFF3038 0.50 +0.3 52,609 + GFF3038 0.50 +1.3 52,610 - GFF3038 0.50 +0.1 52,624 + GFF3038 0.52 -0.5 52,624 + GFF3038 0.52 +0.3 52,670 - GFF3038 0.58 -0.4 52,722 + GFF3038 0.65 +0.2 52,771 - GFF3038 0.72 -1.3 52,820 - GFF3038 0.78 +0.2 52,944 - +0.2 52,987 + +0.2 52,987 + +0.2 52,987 + -0.3 52,991 + +0.3 52,991 + +0.1 52,992 - +0.0 52,992 - -0.6 52,992 - +0.1 52,992 - +1.1 52,995 + +0.5 52,996 - +0.3
Or see this region's nucleotide sequence