Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2799 and GFF2800 are separated by 13 nucleotides GFF2800 and GFF2801 are separated by 170 nucleotides GFF2801 and GFF2802 are separated by 96 nucleotides
GFF2799 - Inner membrane protein, at 322,537 to 323,271
GFF2799
GFF2800 - Membrane-bound lytic murein transglycosylase E (EC 3.2.1.-), at 323,285 to 323,896
GFF2800
GFF2801 - Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13), at 324,067 to 324,981
GFF2801
GFF2802 - Cell volume regulation protein A, at 325,078 to 326,811
GFF2802
Position (kb)
324
325 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 323.282 kb on - strand at 323.461 kb on - strand, within GFF2800 at 323.461 kb on - strand, within GFF2800 at 323.861 kb on + strand at 323.862 kb on - strand at 323.865 kb on - strand at 323.865 kb on - strand at 323.904 kb on - strand at 323.922 kb on + strand at 323.923 kb on - strand at 323.960 kb on + strand at 323.961 kb on - strand at 323.961 kb on - strand at 323.961 kb on - strand at 324.014 kb on + strand at 324.017 kb on + strand at 324.034 kb on - strand at 324.047 kb on - strand at 324.047 kb on - strand at 324.064 kb on - strand at 324.448 kb on - strand, within GFF2801 at 324.483 kb on + strand, within GFF2801 at 324.483 kb on + strand, within GFF2801 at 324.484 kb on - strand, within GFF2801 at 324.699 kb on + strand, within GFF2801 at 324.700 kb on - strand, within GFF2801 at 324.953 kb on + strand at 324.967 kb on - strand at 325.000 kb on + strand at 325.043 kb on - strand at 325.112 kb on + strand at 325.145 kb on + strand at 325.146 kb on - strand at 325.245 kb on + strand at 325.262 kb on - strand, within GFF2802 at 325.262 kb on - strand, within GFF2802 at 325.264 kb on - strand, within GFF2802 at 325.264 kb on - strand, within GFF2802 at 325.587 kb on + strand, within GFF2802 at 325.722 kb on + strand, within GFF2802
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 323,282 - +0.4 323,461 - GFF2800 0.29 +0.2 323,461 - GFF2800 0.29 -0.2 323,861 + -2.1 323,862 - +0.2 323,865 - +0.2 323,865 - -0.1 323,904 - +0.6 323,922 + +0.1 323,923 - -0.5 323,960 + +0.4 323,961 - +1.1 323,961 - +0.1 323,961 - +2.0 324,014 + -0.1 324,017 + -0.6 324,034 - +0.1 324,047 - +0.3 324,047 - +0.8 324,064 - -0.2 324,448 - GFF2801 0.42 -0.3 324,483 + GFF2801 0.45 -0.5 324,483 + GFF2801 0.45 -2.5 324,484 - GFF2801 0.46 -0.1 324,699 + GFF2801 0.69 -0.6 324,700 - GFF2801 0.69 -1.1 324,953 + -0.2 324,967 - +0.6 325,000 + +0.2 325,043 - +0.6 325,112 + +0.3 325,145 + -0.0 325,146 - +0.9 325,245 + +0.1 325,262 - GFF2802 0.11 +0.1 325,262 - GFF2802 0.11 -0.2 325,264 - GFF2802 0.11 +1.2 325,264 - GFF2802 0.11 +0.3 325,587 + GFF2802 0.29 -0.5 325,722 + GFF2802 0.37 -0.0
Or see this region's nucleotide sequence