Experiment: Ceftazidime 5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1530 and grxA are separated by 53 nucleotides grxA and Shew_1532 are separated by 65 nucleotides Shew_1532 and Shew_1533 are separated by 123 nucleotides
Shew_1530: Shew_1530 - porin (RefSeq), at 1,754,711 to 1,755,853
_1530
Shew_1531: grxA - glutaredoxin 1 (RefSeq), at 1,755,907 to 1,756,164
grxA
Shew_1532: Shew_1532 - type III restriction enzyme, res subunit (RefSeq), at 1,756,230 to 1,757,969
_1532
Shew_1533: Shew_1533 - acetyl-CoA synthetase (RefSeq), at 1,758,093 to 1,760,045
_1533
Position (kb)
1756
1757
1758 Strain fitness (log2 ratio)
-1
0
1 at 1755.354 kb on - strand, within Shew_1530 at 1755.730 kb on + strand, within Shew_1530 at 1755.756 kb on - strand at 1755.767 kb on + strand at 1756.001 kb on + strand, within grxA at 1756.124 kb on - strand, within grxA at 1756.206 kb on - strand at 1757.147 kb on + strand, within Shew_1532 at 1757.157 kb on + strand, within Shew_1532 at 1757.160 kb on - strand, within Shew_1532 at 1757.443 kb on + strand, within Shew_1532 at 1757.691 kb on + strand, within Shew_1532 at 1757.964 kb on - strand at 1758.232 kb on + strand at 1758.422 kb on + strand, within Shew_1533 at 1758.632 kb on - strand, within Shew_1533
Per-strain Table
Position Strand Gene LocusTag Fraction Ceftazidime 5 mg/ml remove 1,755,354 - Shew_1530 0.56 +0.4 1,755,730 + Shew_1530 0.89 -1.0 1,755,756 - +0.1 1,755,767 + +0.0 1,756,001 + grxA Shew_1531 0.36 +0.6 1,756,124 - grxA Shew_1531 0.84 +0.7 1,756,206 - +0.7 1,757,147 + Shew_1532 0.53 +0.5 1,757,157 + Shew_1532 0.53 +0.7 1,757,160 - Shew_1532 0.53 +0.0 1,757,443 + Shew_1532 0.70 +0.2 1,757,691 + Shew_1532 0.84 -0.4 1,757,964 - +0.1 1,758,232 + +1.5 1,758,422 + Shew_1533 0.17 +0.3 1,758,632 - Shew_1533 0.28 +0.4
Or see this region's nucleotide sequence