Experiment: acetate 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1973 and Pf6N2E2_1974 are separated by 151 nucleotides Pf6N2E2_1974 and Pf6N2E2_1975 overlap by 1 nucleotides Pf6N2E2_1975 and Pf6N2E2_1976 are separated by 11 nucleotides Pf6N2E2_1976 and Pf6N2E2_1977 are separated by 216 nucleotides
Pf6N2E2_1973 - FIG141751: hypothetical protein in PFGI-1-like cluster, at 2,339,249 to 2,340,397
_1973
Pf6N2E2_1974 - FIG141694: hypothetical protein in PFGI-1-like cluster, at 2,340,549 to 2,341,340
_1974
Pf6N2E2_1975 - Integrase regulator R, at 2,341,340 to 2,341,840
_1975
Pf6N2E2_1976 - Single-stranded DNA-binding protein, at 2,341,852 to 2,342,256
_1976
Pf6N2E2_1977 - DNA topoisomerase III (EC 5.99.1.2) in PFGI-1-like cluster, at 2,342,473 to 2,344,023
_1977
Position (kb)
2341
2342 Strain fitness (log2 ratio)
-1
0
1 at 2340.359 kb on + strand at 2340.359 kb on + strand at 2340.360 kb on - strand at 2340.738 kb on + strand, within Pf6N2E2_1974 at 2340.758 kb on - strand, within Pf6N2E2_1974 at 2340.890 kb on - strand, within Pf6N2E2_1974 at 2340.930 kb on + strand, within Pf6N2E2_1974 at 2340.931 kb on - strand, within Pf6N2E2_1974 at 2340.931 kb on - strand, within Pf6N2E2_1974 at 2340.945 kb on + strand, within Pf6N2E2_1974 at 2341.009 kb on + strand, within Pf6N2E2_1974 at 2341.072 kb on - strand, within Pf6N2E2_1974 at 2341.112 kb on + strand, within Pf6N2E2_1974 at 2341.112 kb on + strand, within Pf6N2E2_1974 at 2341.347 kb on + strand at 2341.347 kb on + strand at 2341.348 kb on - strand at 2341.407 kb on + strand, within Pf6N2E2_1975 at 2341.408 kb on - strand, within Pf6N2E2_1975 at 2341.727 kb on - strand, within Pf6N2E2_1975 at 2341.727 kb on - strand, within Pf6N2E2_1975 at 2341.805 kb on + strand at 2341.806 kb on - strand at 2341.836 kb on + strand at 2341.836 kb on + strand at 2341.837 kb on - strand at 2341.837 kb on - strand at 2341.879 kb on - strand at 2341.879 kb on - strand at 2341.882 kb on - strand at 2342.595 kb on - strand at 2342.695 kb on + strand, within Pf6N2E2_1977 at 2342.696 kb on - strand, within Pf6N2E2_1977 at 2342.696 kb on - strand, within Pf6N2E2_1977 at 2342.808 kb on - strand, within Pf6N2E2_1977
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 80 mM remove 2,340,359 + +0.8 2,340,359 + +0.2 2,340,360 - -0.7 2,340,738 + Pf6N2E2_1974 0.24 +0.1 2,340,758 - Pf6N2E2_1974 0.26 +0.1 2,340,890 - Pf6N2E2_1974 0.43 +0.1 2,340,930 + Pf6N2E2_1974 0.48 -0.2 2,340,931 - Pf6N2E2_1974 0.48 +1.6 2,340,931 - Pf6N2E2_1974 0.48 +0.4 2,340,945 + Pf6N2E2_1974 0.50 -0.1 2,341,009 + Pf6N2E2_1974 0.58 +1.2 2,341,072 - Pf6N2E2_1974 0.66 -0.4 2,341,112 + Pf6N2E2_1974 0.71 -1.3 2,341,112 + Pf6N2E2_1974 0.71 +0.3 2,341,347 + +0.3 2,341,347 + +0.6 2,341,348 - -0.2 2,341,407 + Pf6N2E2_1975 0.13 +0.8 2,341,408 - Pf6N2E2_1975 0.14 +0.3 2,341,727 - Pf6N2E2_1975 0.77 +0.9 2,341,727 - Pf6N2E2_1975 0.77 +0.8 2,341,805 + +0.6 2,341,806 - +0.3 2,341,836 + -0.1 2,341,836 + +0.9 2,341,837 - -0.3 2,341,837 - +0.2 2,341,879 - +0.0 2,341,879 - +0.2 2,341,882 - -0.5 2,342,595 - +0.4 2,342,695 + Pf6N2E2_1977 0.14 +0.3 2,342,696 - Pf6N2E2_1977 0.14 +0.7 2,342,696 - Pf6N2E2_1977 0.14 -0.4 2,342,808 - Pf6N2E2_1977 0.22 +0.5
Or see this region's nucleotide sequence