Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0578 and Shewana3_0579 are separated by 247 nucleotides Shewana3_0579 and Shewana3_0580 are separated by 107 nucleotides Shewana3_0580 and Shewana3_0581 are separated by 199 nucleotides
Shewana3_0578: Shewana3_0578 - serine/threonine protein kinase (RefSeq), at 678,090 to 679,898
_0578
Shewana3_0579: Shewana3_0579 - hypothetical protein (RefSeq), at 680,146 to 680,484
_0579
Shewana3_0580: Shewana3_0580 - thiopurine S-methyltransferase (RefSeq), at 680,592 to 681,248
_0580
Shewana3_0581: Shewana3_0581 - BFD/(2Fe-2S)-binding domain-containing protein (RefSeq), at 681,448 to 681,639
_0581
Position (kb) 680
681 Strain fitness (log2 ratio) -2
-1
0
1 at 679.151 kb on - strand, within Shewana3_0578 at 679.278 kb on - strand, within Shewana3_0578 at 679.419 kb on + strand, within Shewana3_0578 at 679.421 kb on + strand, within Shewana3_0578 at 679.427 kb on - strand, within Shewana3_0578 at 679.448 kb on - strand, within Shewana3_0578 at 679.597 kb on + strand, within Shewana3_0578 at 679.597 kb on + strand, within Shewana3_0578 at 679.599 kb on + strand, within Shewana3_0578 at 679.607 kb on - strand, within Shewana3_0578 at 679.612 kb on + strand, within Shewana3_0578 at 680.138 kb on - strand at 680.268 kb on + strand, within Shewana3_0579 at 680.280 kb on + strand, within Shewana3_0579 at 680.473 kb on + strand at 680.481 kb on - strand at 680.502 kb on - strand at 680.909 kb on - strand, within Shewana3_0580 at 680.939 kb on + strand, within Shewana3_0580 at 680.947 kb on - strand, within Shewana3_0580 at 681.035 kb on - strand, within Shewana3_0580
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 679,151 - Shewana3_0578 0.59 +0.4 679,278 - Shewana3_0578 0.66 -1.1 679,419 + Shewana3_0578 0.73 +0.3 679,421 + Shewana3_0578 0.74 -1.9 679,427 - Shewana3_0578 0.74 -1.0 679,448 - Shewana3_0578 0.75 -0.8 679,597 + Shewana3_0578 0.83 -0.8 679,597 + Shewana3_0578 0.83 -0.3 679,599 + Shewana3_0578 0.83 -0.9 679,607 - Shewana3_0578 0.84 -1.1 679,612 + Shewana3_0578 0.84 -0.5 680,138 - +1.6 680,268 + Shewana3_0579 0.36 -1.7 680,280 + Shewana3_0579 0.40 -0.6 680,473 + -0.3 680,481 - +0.1 680,502 - -2.4 680,909 - Shewana3_0580 0.48 -1.7 680,939 + Shewana3_0580 0.53 -0.6 680,947 - Shewana3_0580 0.54 -1.2 681,035 - Shewana3_0580 0.67 -0.9
Or see this region's nucleotide sequence