Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_93 and Pf6N2E2_94 are separated by 294 nucleotides Pf6N2E2_94 and Pf6N2E2_95 are separated by 109 nucleotides
Pf6N2E2_93 - probable membrane protein STY3606, at 138,950 to 139,378
_93
Pf6N2E2_94 - Cyn operon transcriptional activator, at 139,673 to 140,587
_94
Pf6N2E2_95 - putative oxygenase (putative secreted protein), at 140,697 to 141,782
_95
Position (kb)
139
140
141 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 138.679 kb on - strand at 138.722 kb on + strand at 138.722 kb on + strand at 138.722 kb on + strand at 138.722 kb on + strand at 138.722 kb on + strand at 138.722 kb on + strand at 138.722 kb on + strand at 138.722 kb on + strand at 138.722 kb on + strand at 138.722 kb on + strand at 138.723 kb on - strand at 138.723 kb on - strand at 138.734 kb on + strand at 138.734 kb on + strand at 138.735 kb on - strand at 138.847 kb on + strand at 138.847 kb on + strand at 138.847 kb on + strand at 139.008 kb on + strand, within Pf6N2E2_93 at 139.008 kb on + strand, within Pf6N2E2_93 at 139.009 kb on - strand, within Pf6N2E2_93 at 139.011 kb on + strand, within Pf6N2E2_93 at 139.012 kb on - strand, within Pf6N2E2_93 at 139.012 kb on - strand, within Pf6N2E2_93 at 139.017 kb on + strand, within Pf6N2E2_93 at 139.017 kb on + strand, within Pf6N2E2_93 at 139.017 kb on + strand, within Pf6N2E2_93 at 139.017 kb on + strand, within Pf6N2E2_93 at 139.017 kb on + strand, within Pf6N2E2_93 at 139.018 kb on - strand, within Pf6N2E2_93 at 139.018 kb on - strand, within Pf6N2E2_93 at 139.018 kb on - strand, within Pf6N2E2_93 at 139.035 kb on + strand, within Pf6N2E2_93 at 139.035 kb on + strand, within Pf6N2E2_93 at 139.035 kb on + strand, within Pf6N2E2_93 at 139.036 kb on - strand, within Pf6N2E2_93 at 139.036 kb on - strand, within Pf6N2E2_93 at 139.036 kb on - strand, within Pf6N2E2_93 at 139.036 kb on - strand, within Pf6N2E2_93 at 139.036 kb on - strand, within Pf6N2E2_93 at 139.036 kb on - strand, within Pf6N2E2_93 at 139.036 kb on - strand, within Pf6N2E2_93 at 139.036 kb on - strand, within Pf6N2E2_93 at 139.036 kb on - strand, within Pf6N2E2_93 at 139.067 kb on - strand, within Pf6N2E2_93 at 139.067 kb on - strand, within Pf6N2E2_93 at 139.188 kb on + strand, within Pf6N2E2_93 at 139.189 kb on - strand, within Pf6N2E2_93 at 139.193 kb on - strand, within Pf6N2E2_93 at 139.193 kb on - strand, within Pf6N2E2_93 at 139.274 kb on - strand, within Pf6N2E2_93 at 139.276 kb on - strand, within Pf6N2E2_93 at 139.341 kb on + strand at 139.342 kb on - strand at 139.342 kb on - strand at 139.342 kb on - strand at 139.346 kb on - strand at 139.358 kb on + strand at 139.358 kb on + strand at 139.358 kb on + strand at 139.359 kb on - strand at 139.503 kb on - strand at 139.503 kb on - strand at 139.587 kb on - strand at 139.659 kb on + strand at 139.659 kb on + strand at 139.660 kb on - strand at 139.797 kb on - strand, within Pf6N2E2_94 at 139.797 kb on - strand, within Pf6N2E2_94 at 140.071 kb on - strand, within Pf6N2E2_94 at 140.085 kb on + strand, within Pf6N2E2_94 at 140.086 kb on - strand, within Pf6N2E2_94 at 140.086 kb on - strand, within Pf6N2E2_94 at 140.100 kb on + strand, within Pf6N2E2_94 at 140.100 kb on + strand, within Pf6N2E2_94 at 140.101 kb on - strand, within Pf6N2E2_94 at 140.575 kb on + strand at 140.575 kb on + strand at 140.575 kb on + strand at 140.614 kb on + strand at 140.615 kb on - strand at 140.615 kb on - strand at 140.615 kb on - strand at 140.675 kb on + strand at 140.675 kb on + strand at 140.676 kb on - strand at 140.676 kb on - strand at 140.676 kb on - strand at 140.676 kb on - strand at 140.676 kb on - strand at 140.679 kb on + strand at 140.680 kb on - strand at 140.680 kb on - strand at 140.696 kb on + strand at 140.696 kb on + strand at 140.696 kb on + strand at 140.696 kb on + strand at 140.697 kb on - strand at 140.697 kb on - strand at 140.697 kb on - strand at 140.697 kb on - strand at 140.809 kb on + strand, within Pf6N2E2_95 at 140.810 kb on - strand at 141.033 kb on + strand, within Pf6N2E2_95 at 141.148 kb on + strand, within Pf6N2E2_95 at 141.148 kb on + strand, within Pf6N2E2_95 at 141.149 kb on - strand, within Pf6N2E2_95 at 141.149 kb on - strand, within Pf6N2E2_95 at 141.149 kb on - strand, within Pf6N2E2_95 at 141.149 kb on - strand, within Pf6N2E2_95 at 141.149 kb on - strand, within Pf6N2E2_95 at 141.149 kb on - strand, within Pf6N2E2_95 at 141.292 kb on - strand, within Pf6N2E2_95 at 141.507 kb on + strand, within Pf6N2E2_95 at 141.507 kb on + strand, within Pf6N2E2_95
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 138,679 - +0.0 138,722 + +0.7 138,722 + +0.0 138,722 + +0.6 138,722 + +0.2 138,722 + +1.8 138,722 + -0.2 138,722 + +0.1 138,722 + +0.3 138,722 + +0.3 138,722 + +0.2 138,723 - -0.1 138,723 - -0.5 138,734 + +0.5 138,734 + -0.1 138,735 - +0.5 138,847 + +0.4 138,847 + -0.6 138,847 + +0.3 139,008 + Pf6N2E2_93 0.14 +0.7 139,008 + Pf6N2E2_93 0.14 -0.3 139,009 - Pf6N2E2_93 0.14 -1.4 139,011 + Pf6N2E2_93 0.14 +1.7 139,012 - Pf6N2E2_93 0.14 -0.0 139,012 - Pf6N2E2_93 0.14 +0.7 139,017 + Pf6N2E2_93 0.16 +0.0 139,017 + Pf6N2E2_93 0.16 +0.4 139,017 + Pf6N2E2_93 0.16 -0.1 139,017 + Pf6N2E2_93 0.16 -1.2 139,017 + Pf6N2E2_93 0.16 -0.2 139,018 - Pf6N2E2_93 0.16 -0.8 139,018 - Pf6N2E2_93 0.16 +0.2 139,018 - Pf6N2E2_93 0.16 +0.6 139,035 + Pf6N2E2_93 0.20 -0.1 139,035 + Pf6N2E2_93 0.20 -0.1 139,035 + Pf6N2E2_93 0.20 -0.1 139,036 - Pf6N2E2_93 0.20 +0.0 139,036 - Pf6N2E2_93 0.20 +0.6 139,036 - Pf6N2E2_93 0.20 -0.4 139,036 - Pf6N2E2_93 0.20 +0.0 139,036 - Pf6N2E2_93 0.20 -0.4 139,036 - Pf6N2E2_93 0.20 -0.3 139,036 - Pf6N2E2_93 0.20 +0.7 139,036 - Pf6N2E2_93 0.20 +1.6 139,036 - Pf6N2E2_93 0.20 -2.2 139,067 - Pf6N2E2_93 0.27 +0.4 139,067 - Pf6N2E2_93 0.27 -0.8 139,188 + Pf6N2E2_93 0.55 +0.4 139,189 - Pf6N2E2_93 0.56 +0.4 139,193 - Pf6N2E2_93 0.57 +0.6 139,193 - Pf6N2E2_93 0.57 +0.3 139,274 - Pf6N2E2_93 0.76 -0.5 139,276 - Pf6N2E2_93 0.76 +0.2 139,341 + +1.0 139,342 - +0.3 139,342 - -0.9 139,342 - -0.9 139,346 - +1.6 139,358 + +0.2 139,358 + +1.0 139,358 + +0.2 139,359 - -0.8 139,503 - -0.1 139,503 - -0.3 139,587 - +0.4 139,659 + -1.3 139,659 + +0.2 139,660 - -0.7 139,797 - Pf6N2E2_94 0.14 +0.0 139,797 - Pf6N2E2_94 0.14 -0.4 140,071 - Pf6N2E2_94 0.43 -0.8 140,085 + Pf6N2E2_94 0.45 -1.3 140,086 - Pf6N2E2_94 0.45 +0.4 140,086 - Pf6N2E2_94 0.45 -0.2 140,100 + Pf6N2E2_94 0.47 -0.7 140,100 + Pf6N2E2_94 0.47 -1.3 140,101 - Pf6N2E2_94 0.47 -0.4 140,575 + -0.6 140,575 + -0.7 140,575 + -2.0 140,614 + -0.1 140,615 - -0.4 140,615 - -0.3 140,615 - +1.2 140,675 + +0.1 140,675 + -0.6 140,676 - -0.2 140,676 - -0.4 140,676 - -0.5 140,676 - -0.4 140,676 - +0.4 140,679 + +0.6 140,680 - +0.2 140,680 - -0.5 140,696 + -1.5 140,696 + +0.7 140,696 + +0.0 140,696 + -0.1 140,697 - +1.2 140,697 - +0.5 140,697 - -0.0 140,697 - -0.4 140,809 + Pf6N2E2_95 0.10 +0.0 140,810 - +3.2 141,033 + Pf6N2E2_95 0.31 +0.2 141,148 + Pf6N2E2_95 0.42 -1.0 141,148 + Pf6N2E2_95 0.42 +0.3 141,149 - Pf6N2E2_95 0.42 +0.3 141,149 - Pf6N2E2_95 0.42 -0.1 141,149 - Pf6N2E2_95 0.42 +0.4 141,149 - Pf6N2E2_95 0.42 -0.3 141,149 - Pf6N2E2_95 0.42 +0.3 141,149 - Pf6N2E2_95 0.42 +0.8 141,292 - Pf6N2E2_95 0.55 +0.2 141,507 + Pf6N2E2_95 0.75 +0.2 141,507 + Pf6N2E2_95 0.75 -0.8
Or see this region's nucleotide sequence