Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_734

Experiment: Spectinomycin 0.0125 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_733 and Pf6N2E2_734 are separated by 25 nucleotidesPf6N2E2_734 and Pf6N2E2_735 are separated by 277 nucleotides Pf6N2E2_733 - Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8), at 840,991 to 841,959 _733 Pf6N2E2_734 - Molybdenum cofactor biosynthesis protein MoaA, at 841,985 to 842,977 _734 Pf6N2E2_735 - Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2), at 843,255 to 845,270 _735 Position (kb) 841 842 843Strain fitness (log2 ratio) -2 -1 0 1at 841.246 kb on + strand, within Pf6N2E2_733at 841.247 kb on - strand, within Pf6N2E2_733at 841.454 kb on - strand, within Pf6N2E2_733at 841.454 kb on - strand, within Pf6N2E2_733at 841.454 kb on - strand, within Pf6N2E2_733at 841.882 kb on + strandat 842.073 kb on - strandat 842.517 kb on - strand, within Pf6N2E2_734at 842.684 kb on + strand, within Pf6N2E2_734at 842.684 kb on + strand, within Pf6N2E2_734at 842.684 kb on + strand, within Pf6N2E2_734at 842.685 kb on - strand, within Pf6N2E2_734at 842.975 kb on + strandat 842.981 kb on + strandat 842.981 kb on + strandat 842.981 kb on + strandat 842.981 kb on + strandat 843.088 kb on + strandat 843.088 kb on + strandat 843.088 kb on + strandat 843.088 kb on + strandat 843.089 kb on - strandat 843.128 kb on - strandat 843.128 kb on - strandat 843.233 kb on - strandat 843.234 kb on + strandat 843.234 kb on + strandat 843.235 kb on - strandat 843.235 kb on - strandat 843.235 kb on - strandat 843.238 kb on + strandat 843.238 kb on + strandat 843.238 kb on + strandat 843.238 kb on + strandat 843.239 kb on - strandat 843.239 kb on - strandat 843.239 kb on - strandat 843.239 kb on - strandat 843.239 kb on - strandat 843.239 kb on - strandat 843.285 kb on - strandat 843.519 kb on + strand, within Pf6N2E2_735at 843.519 kb on + strand, within Pf6N2E2_735at 843.519 kb on + strand, within Pf6N2E2_735at 843.520 kb on - strand, within Pf6N2E2_735at 843.520 kb on - strand, within Pf6N2E2_735at 843.648 kb on + strand, within Pf6N2E2_735at 843.676 kb on + strand, within Pf6N2E2_735at 843.699 kb on + strand, within Pf6N2E2_735at 843.699 kb on + strand, within Pf6N2E2_735at 843.699 kb on + strand, within Pf6N2E2_735at 843.700 kb on - strand, within Pf6N2E2_735at 843.717 kb on + strand, within Pf6N2E2_735at 843.717 kb on + strand, within Pf6N2E2_735at 843.718 kb on - strand, within Pf6N2E2_735at 843.718 kb on - strand, within Pf6N2E2_735at 843.762 kb on - strand, within Pf6N2E2_735at 843.967 kb on - strand, within Pf6N2E2_735at 843.967 kb on - strand, within Pf6N2E2_735at 843.967 kb on - strand, within Pf6N2E2_735at 843.967 kb on - strand, within Pf6N2E2_735at 843.967 kb on - strand, within Pf6N2E2_735

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml
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841,246 + Pf6N2E2_733 0.26 +0.5
841,247 - Pf6N2E2_733 0.26 -0.2
841,454 - Pf6N2E2_733 0.48 -0.0
841,454 - Pf6N2E2_733 0.48 +0.1
841,454 - Pf6N2E2_733 0.48 +0.3
841,882 + -0.1
842,073 - +0.7
842,517 - Pf6N2E2_734 0.54 -2.1
842,684 + Pf6N2E2_734 0.70 -0.3
842,684 + Pf6N2E2_734 0.70 -0.8
842,684 + Pf6N2E2_734 0.70 -0.3
842,685 - Pf6N2E2_734 0.70 -0.7
842,975 + -0.1
842,981 + -0.3
842,981 + +0.2
842,981 + -1.9
842,981 + +0.9
843,088 + +0.3
843,088 + +0.2
843,088 + -0.5
843,088 + +0.1
843,089 - -0.4
843,128 - -0.1
843,128 - -0.4
843,233 - +0.5
843,234 + -1.1
843,234 + +0.0
843,235 - +0.0
843,235 - -0.7
843,235 - +0.6
843,238 + -0.5
843,238 + +0.2
843,238 + -0.5
843,238 + +0.8
843,239 - +1.4
843,239 - -0.7
843,239 - -0.4
843,239 - -0.6
843,239 - +0.4
843,239 - +0.2
843,285 - -0.7
843,519 + Pf6N2E2_735 0.13 -0.4
843,519 + Pf6N2E2_735 0.13 +0.3
843,519 + Pf6N2E2_735 0.13 -0.4
843,520 - Pf6N2E2_735 0.13 +0.7
843,520 - Pf6N2E2_735 0.13 +0.5
843,648 + Pf6N2E2_735 0.19 -0.7
843,676 + Pf6N2E2_735 0.21 +1.5
843,699 + Pf6N2E2_735 0.22 +0.1
843,699 + Pf6N2E2_735 0.22 -0.9
843,699 + Pf6N2E2_735 0.22 -0.0
843,700 - Pf6N2E2_735 0.22 +0.6
843,717 + Pf6N2E2_735 0.23 -0.4
843,717 + Pf6N2E2_735 0.23 -0.7
843,718 - Pf6N2E2_735 0.23 +0.9
843,718 - Pf6N2E2_735 0.23 -0.9
843,762 - Pf6N2E2_735 0.25 +0.1
843,967 - Pf6N2E2_735 0.35 +1.0
843,967 - Pf6N2E2_735 0.35 +0.7
843,967 - Pf6N2E2_735 0.35 +0.2
843,967 - Pf6N2E2_735 0.35 +0.6
843,967 - Pf6N2E2_735 0.35 +1.2

Or see this region's nucleotide sequence