Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_620 and Pf6N2E2_621 overlap by 4 nucleotides Pf6N2E2_621 and Pf6N2E2_622 overlap by 4 nucleotides
Pf6N2E2_620 - BatD, at 705,629 to 707,266
_620
Pf6N2E2_621 - TPR domain protein in aerotolerance operon, at 707,263 to 708,999
_621
Pf6N2E2_622 - BatA (Bacteroides aerotolerance operon), at 708,996 to 710,075
_622
Position (kb) 707
708
709 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 706.488 kb on + strand, within Pf6N2E2_620 at 706.653 kb on + strand, within Pf6N2E2_620 at 706.653 kb on + strand, within Pf6N2E2_620 at 706.654 kb on - strand, within Pf6N2E2_620 at 706.654 kb on - strand, within Pf6N2E2_620 at 706.710 kb on + strand, within Pf6N2E2_620 at 706.782 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.837 kb on - strand, within Pf6N2E2_620 at 706.837 kb on - strand, within Pf6N2E2_620 at 706.837 kb on - strand, within Pf6N2E2_620 at 706.837 kb on - strand, within Pf6N2E2_620 at 707.247 kb on + strand at 707.247 kb on + strand at 707.248 kb on - strand at 707.248 kb on - strand at 707.313 kb on + strand at 707.458 kb on + strand, within Pf6N2E2_621 at 707.458 kb on + strand, within Pf6N2E2_621 at 707.458 kb on + strand, within Pf6N2E2_621 at 707.459 kb on - strand, within Pf6N2E2_621 at 707.723 kb on + strand, within Pf6N2E2_621 at 707.723 kb on + strand, within Pf6N2E2_621 at 707.723 kb on + strand, within Pf6N2E2_621 at 707.723 kb on + strand, within Pf6N2E2_621 at 707.780 kb on + strand, within Pf6N2E2_621 at 707.780 kb on + strand, within Pf6N2E2_621 at 707.781 kb on - strand, within Pf6N2E2_621 at 707.995 kb on + strand, within Pf6N2E2_621 at 707.995 kb on + strand, within Pf6N2E2_621 at 707.996 kb on - strand, within Pf6N2E2_621 at 708.275 kb on + strand, within Pf6N2E2_621 at 708.567 kb on + strand, within Pf6N2E2_621 at 708.634 kb on + strand, within Pf6N2E2_621 at 708.634 kb on + strand, within Pf6N2E2_621 at 708.635 kb on - strand, within Pf6N2E2_621 at 708.861 kb on + strand at 708.862 kb on - strand at 708.862 kb on - strand at 708.950 kb on - strand at 708.950 kb on - strand at 708.958 kb on + strand at 708.959 kb on - strand at 709.165 kb on + strand, within Pf6N2E2_622 at 709.166 kb on - strand, within Pf6N2E2_622 at 709.245 kb on + strand, within Pf6N2E2_622 at 709.639 kb on + strand, within Pf6N2E2_622 at 709.639 kb on + strand, within Pf6N2E2_622 at 709.640 kb on - strand, within Pf6N2E2_622
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 706,488 + Pf6N2E2_620 0.52 -2.7 706,653 + Pf6N2E2_620 0.63 -0.8 706,653 + Pf6N2E2_620 0.63 -2.4 706,654 - Pf6N2E2_620 0.63 -0.9 706,654 - Pf6N2E2_620 0.63 -1.9 706,710 + Pf6N2E2_620 0.66 -1.9 706,782 + Pf6N2E2_620 0.70 -1.7 706,836 + Pf6N2E2_620 0.74 -1.6 706,836 + Pf6N2E2_620 0.74 -1.9 706,836 + Pf6N2E2_620 0.74 -1.5 706,836 + Pf6N2E2_620 0.74 -1.9 706,836 + Pf6N2E2_620 0.74 -2.2 706,836 + Pf6N2E2_620 0.74 -2.0 706,836 + Pf6N2E2_620 0.74 -1.9 706,836 + Pf6N2E2_620 0.74 -1.4 706,836 + Pf6N2E2_620 0.74 -4.1 706,836 + Pf6N2E2_620 0.74 -2.3 706,837 - Pf6N2E2_620 0.74 -1.3 706,837 - Pf6N2E2_620 0.74 +0.0 706,837 - Pf6N2E2_620 0.74 -0.8 706,837 - Pf6N2E2_620 0.74 -1.9 707,247 + -0.7 707,247 + -2.1 707,248 - -2.8 707,248 - -0.7 707,313 + -0.9 707,458 + Pf6N2E2_621 0.11 -0.6 707,458 + Pf6N2E2_621 0.11 -0.6 707,458 + Pf6N2E2_621 0.11 -1.8 707,459 - Pf6N2E2_621 0.11 -2.3 707,723 + Pf6N2E2_621 0.26 -0.8 707,723 + Pf6N2E2_621 0.26 -1.1 707,723 + Pf6N2E2_621 0.26 -2.9 707,723 + Pf6N2E2_621 0.26 -3.2 707,780 + Pf6N2E2_621 0.30 -1.7 707,780 + Pf6N2E2_621 0.30 -1.6 707,781 - Pf6N2E2_621 0.30 -2.4 707,995 + Pf6N2E2_621 0.42 -1.9 707,995 + Pf6N2E2_621 0.42 -2.9 707,996 - Pf6N2E2_621 0.42 -4.7 708,275 + Pf6N2E2_621 0.58 -2.8 708,567 + Pf6N2E2_621 0.75 -3.7 708,634 + Pf6N2E2_621 0.79 -2.9 708,634 + Pf6N2E2_621 0.79 -3.3 708,635 - Pf6N2E2_621 0.79 -1.5 708,861 + -0.9 708,862 - -2.0 708,862 - -0.9 708,950 - -1.9 708,950 - -0.8 708,958 + +0.1 708,959 - -2.1 709,165 + Pf6N2E2_622 0.16 -1.9 709,166 - Pf6N2E2_622 0.16 -1.4 709,245 + Pf6N2E2_622 0.23 -1.7 709,639 + Pf6N2E2_622 0.60 -2.0 709,639 + Pf6N2E2_622 0.60 -1.2 709,640 - Pf6N2E2_622 0.60 -1.9
Or see this region's nucleotide sequence