Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5343 and Pf6N2E2_5344 are separated by 102 nucleotides Pf6N2E2_5344 and Pf6N2E2_5345 overlap by 1 nucleotides Pf6N2E2_5345 and Pf6N2E2_5346 are separated by 341 nucleotides
Pf6N2E2_5343 - Phosphoheptose isomerase (EC 5.3.1.-), at 6,137,005 to 6,137,598
_5343
Pf6N2E2_5344 - Predicted endonuclease distantly related to archaeal Holliday junction resolvase, at 6,137,701 to 6,138,063
_5344
Pf6N2E2_5345 - LppC putative lipoprotein, at 6,138,063 to 6,139,874
_5345
Pf6N2E2_5346 - rRNA small subunit methyltransferase I, at 6,140,216 to 6,141,088
_5346
Position (kb)
6138
6139
6140 Strain fitness (log2 ratio)
-2
-1
0
1 at 6137.611 kb on - strand at 6137.611 kb on - strand at 6137.611 kb on - strand at 6137.611 kb on - strand at 6137.611 kb on - strand at 6137.611 kb on - strand at 6137.615 kb on - strand at 6137.814 kb on - strand, within Pf6N2E2_5344 at 6137.884 kb on - strand, within Pf6N2E2_5344 at 6137.912 kb on - strand, within Pf6N2E2_5344 at 6137.912 kb on - strand, within Pf6N2E2_5344 at 6137.915 kb on - strand, within Pf6N2E2_5344 at 6137.915 kb on - strand, within Pf6N2E2_5344 at 6137.915 kb on - strand, within Pf6N2E2_5344 at 6137.915 kb on - strand, within Pf6N2E2_5344 at 6137.915 kb on - strand, within Pf6N2E2_5344 at 6137.915 kb on - strand, within Pf6N2E2_5344 at 6137.920 kb on - strand, within Pf6N2E2_5344 at 6137.920 kb on - strand, within Pf6N2E2_5344 at 6138.132 kb on + strand at 6138.237 kb on + strand at 6138.237 kb on + strand at 6138.250 kb on + strand, within Pf6N2E2_5345 at 6138.250 kb on + strand, within Pf6N2E2_5345 at 6138.370 kb on + strand, within Pf6N2E2_5345 at 6138.371 kb on - strand, within Pf6N2E2_5345 at 6138.416 kb on - strand, within Pf6N2E2_5345 at 6138.436 kb on + strand, within Pf6N2E2_5345 at 6138.437 kb on - strand, within Pf6N2E2_5345 at 6138.847 kb on + strand, within Pf6N2E2_5345 at 6138.847 kb on + strand, within Pf6N2E2_5345 at 6138.847 kb on + strand, within Pf6N2E2_5345 at 6138.848 kb on - strand, within Pf6N2E2_5345 at 6138.848 kb on - strand, within Pf6N2E2_5345 at 6138.848 kb on - strand, within Pf6N2E2_5345 at 6138.996 kb on + strand, within Pf6N2E2_5345 at 6138.997 kb on + strand, within Pf6N2E2_5345 at 6138.997 kb on + strand, within Pf6N2E2_5345 at 6138.998 kb on - strand, within Pf6N2E2_5345 at 6139.043 kb on - strand, within Pf6N2E2_5345 at 6139.043 kb on - strand, within Pf6N2E2_5345 at 6139.876 kb on + strand at 6139.876 kb on + strand at 6139.876 kb on + strand at 6139.876 kb on + strand at 6139.876 kb on + strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.902 kb on + strand at 6139.916 kb on - strand at 6139.916 kb on - strand at 6139.944 kb on - strand at 6139.965 kb on + strand at 6139.966 kb on - strand at 6139.966 kb on - strand at 6140.069 kb on + strand at 6140.069 kb on + strand at 6140.069 kb on + strand at 6140.070 kb on - strand at 6140.098 kb on + strand at 6140.179 kb on + strand at 6140.179 kb on + strand at 6140.179 kb on + strand at 6140.194 kb on + strand at 6140.208 kb on + strand at 6140.208 kb on + strand at 6140.209 kb on - strand at 6140.258 kb on + strand at 6140.258 kb on + strand at 6140.258 kb on + strand at 6140.259 kb on - strand at 6140.259 kb on - strand at 6140.294 kb on - strand at 6140.689 kb on + strand, within Pf6N2E2_5346 at 6140.689 kb on + strand, within Pf6N2E2_5346 at 6140.689 kb on + strand, within Pf6N2E2_5346 at 6140.690 kb on - strand, within Pf6N2E2_5346 at 6140.721 kb on - strand, within Pf6N2E2_5346
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 6,137,611 - -0.2 6,137,611 - +0.3 6,137,611 - -0.5 6,137,611 - +0.5 6,137,611 - -0.7 6,137,611 - +0.2 6,137,615 - -2.0 6,137,814 - Pf6N2E2_5344 0.31 +0.1 6,137,884 - Pf6N2E2_5344 0.50 -0.5 6,137,912 - Pf6N2E2_5344 0.58 +0.3 6,137,912 - Pf6N2E2_5344 0.58 +0.1 6,137,915 - Pf6N2E2_5344 0.59 -1.4 6,137,915 - Pf6N2E2_5344 0.59 +0.6 6,137,915 - Pf6N2E2_5344 0.59 +0.1 6,137,915 - Pf6N2E2_5344 0.59 +0.6 6,137,915 - Pf6N2E2_5344 0.59 -0.2 6,137,915 - Pf6N2E2_5344 0.59 -1.1 6,137,920 - Pf6N2E2_5344 0.60 +1.0 6,137,920 - Pf6N2E2_5344 0.60 -0.4 6,138,132 + +0.9 6,138,237 + -0.2 6,138,237 + -0.4 6,138,250 + Pf6N2E2_5345 0.10 +0.2 6,138,250 + Pf6N2E2_5345 0.10 -0.4 6,138,370 + Pf6N2E2_5345 0.17 -0.0 6,138,371 - Pf6N2E2_5345 0.17 -0.1 6,138,416 - Pf6N2E2_5345 0.19 -0.6 6,138,436 + Pf6N2E2_5345 0.21 -0.6 6,138,437 - Pf6N2E2_5345 0.21 -0.4 6,138,847 + Pf6N2E2_5345 0.43 -1.6 6,138,847 + Pf6N2E2_5345 0.43 -0.3 6,138,847 + Pf6N2E2_5345 0.43 -0.4 6,138,848 - Pf6N2E2_5345 0.43 -0.5 6,138,848 - Pf6N2E2_5345 0.43 -0.5 6,138,848 - Pf6N2E2_5345 0.43 -0.8 6,138,996 + Pf6N2E2_5345 0.51 -2.0 6,138,997 + Pf6N2E2_5345 0.52 -0.4 6,138,997 + Pf6N2E2_5345 0.52 -0.2 6,138,998 - Pf6N2E2_5345 0.52 -0.2 6,139,043 - Pf6N2E2_5345 0.54 -0.5 6,139,043 - Pf6N2E2_5345 0.54 -0.2 6,139,876 + -0.1 6,139,876 + -0.1 6,139,876 + -0.8 6,139,876 + -1.1 6,139,876 + +0.5 6,139,877 - -0.9 6,139,877 - -0.2 6,139,877 - -0.2 6,139,877 - -2.0 6,139,877 - -1.4 6,139,877 - -0.3 6,139,877 - -0.4 6,139,877 - -0.9 6,139,877 - -0.0 6,139,877 - -0.4 6,139,877 - -0.8 6,139,877 - -0.5 6,139,877 - -0.4 6,139,902 + -0.8 6,139,916 - +0.1 6,139,916 - +0.3 6,139,944 - +1.0 6,139,965 + -0.7 6,139,966 - +0.9 6,139,966 - -1.8 6,140,069 + -0.8 6,140,069 + +0.2 6,140,069 + -1.2 6,140,070 - -0.1 6,140,098 + -0.0 6,140,179 + -0.3 6,140,179 + +0.8 6,140,179 + -0.1 6,140,194 + -0.4 6,140,208 + +0.2 6,140,208 + +1.0 6,140,209 - +0.6 6,140,258 + +0.0 6,140,258 + +0.0 6,140,258 + -0.2 6,140,259 - -0.4 6,140,259 - +0.6 6,140,294 - +0.5 6,140,689 + Pf6N2E2_5346 0.54 +1.1 6,140,689 + Pf6N2E2_5346 0.54 +1.1 6,140,689 + Pf6N2E2_5346 0.54 -0.1 6,140,690 - Pf6N2E2_5346 0.54 +0.7 6,140,721 - Pf6N2E2_5346 0.58 -0.9
Or see this region's nucleotide sequence