Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1237 and Pf6N2E2_1238 are separated by 283 nucleotides Pf6N2E2_1238 and Pf6N2E2_1239 are separated by 270 nucleotides Pf6N2E2_1239 and Pf6N2E2_6105 are separated by 124 nucleotides Pf6N2E2_6105 and Pf6N2E2_1240 are separated by 127 nucleotides
Pf6N2E2_1237 - Putative transport protein, at 1,450,034 to 1,451,497
_1237
Pf6N2E2_1238 - hypothetical protein, at 1,451,781 to 1,451,969
_1238
Pf6N2E2_1239 - Putative TEGT family carrier/transport protein, at 1,452,240 to 1,452,911
_1239
Pf6N2E2_6105 - tRNA-Ser-TGA, at 1,453,036 to 1,453,120
_6105
Pf6N2E2_1240 - Membrane protease family protein y2843, at 1,453,248 to 1,454,102
_1240
Position (kb)
1452
1453 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1451.601 kb on + strand at 1451.608 kb on - strand at 1451.620 kb on + strand at 1451.620 kb on + strand at 1451.717 kb on + strand at 1451.717 kb on + strand at 1451.717 kb on + strand at 1451.717 kb on + strand at 1451.717 kb on + strand at 1451.718 kb on - strand at 1451.718 kb on - strand at 1451.740 kb on + strand at 1451.899 kb on + strand, within Pf6N2E2_1238 at 1451.935 kb on + strand, within Pf6N2E2_1238 at 1451.935 kb on + strand, within Pf6N2E2_1238 at 1451.967 kb on + strand at 1451.967 kb on + strand at 1451.967 kb on + strand at 1451.967 kb on + strand at 1451.968 kb on - strand at 1451.968 kb on - strand at 1451.988 kb on + strand at 1451.989 kb on - strand at 1452.069 kb on - strand at 1452.084 kb on + strand at 1452.084 kb on + strand at 1452.084 kb on + strand at 1452.084 kb on + strand at 1452.084 kb on + strand at 1452.084 kb on + strand at 1452.084 kb on + strand at 1452.084 kb on + strand at 1452.084 kb on + strand at 1452.085 kb on - strand at 1452.085 kb on - strand at 1452.085 kb on - strand at 1452.085 kb on - strand at 1452.085 kb on - strand at 1452.085 kb on - strand at 1452.085 kb on - strand at 1452.085 kb on - strand at 1452.085 kb on - strand at 1452.085 kb on - strand at 1452.086 kb on + strand at 1452.130 kb on + strand at 1452.130 kb on + strand at 1452.130 kb on + strand at 1452.131 kb on - strand at 1452.189 kb on - strand at 1452.296 kb on - strand at 1452.296 kb on - strand at 1452.303 kb on + strand at 1452.303 kb on + strand at 1452.304 kb on - strand at 1452.304 kb on - strand at 1452.307 kb on + strand at 1452.308 kb on - strand, within Pf6N2E2_1239 at 1452.308 kb on - strand, within Pf6N2E2_1239 at 1452.308 kb on - strand, within Pf6N2E2_1239 at 1452.308 kb on - strand, within Pf6N2E2_1239 at 1452.308 kb on - strand, within Pf6N2E2_1239 at 1452.308 kb on - strand, within Pf6N2E2_1239 at 1452.308 kb on - strand, within Pf6N2E2_1239 at 1452.308 kb on - strand, within Pf6N2E2_1239 at 1452.308 kb on - strand, within Pf6N2E2_1239 at 1452.332 kb on - strand, within Pf6N2E2_1239 at 1452.332 kb on - strand, within Pf6N2E2_1239 at 1452.332 kb on - strand, within Pf6N2E2_1239 at 1452.332 kb on - strand, within Pf6N2E2_1239 at 1452.332 kb on - strand, within Pf6N2E2_1239 at 1452.332 kb on - strand, within Pf6N2E2_1239 at 1452.332 kb on - strand, within Pf6N2E2_1239 at 1452.332 kb on - strand, within Pf6N2E2_1239 at 1452.335 kb on + strand, within Pf6N2E2_1239 at 1452.556 kb on - strand, within Pf6N2E2_1239 at 1452.556 kb on - strand, within Pf6N2E2_1239 at 1452.556 kb on - strand, within Pf6N2E2_1239 at 1452.617 kb on - strand, within Pf6N2E2_1239 at 1452.787 kb on + strand, within Pf6N2E2_1239 at 1452.832 kb on + strand, within Pf6N2E2_1239 at 1452.832 kb on + strand, within Pf6N2E2_1239 at 1452.833 kb on - strand, within Pf6N2E2_1239 at 1452.955 kb on + strand at 1452.955 kb on + strand at 1452.955 kb on + strand at 1452.956 kb on - strand at 1452.956 kb on - strand at 1452.969 kb on + strand at 1452.977 kb on + strand at 1452.977 kb on + strand at 1452.977 kb on + strand at 1452.977 kb on + strand at 1452.978 kb on - strand at 1452.982 kb on - strand at 1452.982 kb on - strand at 1453.212 kb on - strand at 1453.256 kb on + strand at 1453.257 kb on - strand at 1453.326 kb on + strand at 1453.355 kb on - strand, within Pf6N2E2_1240 at 1453.419 kb on - strand, within Pf6N2E2_1240 at 1453.464 kb on + strand, within Pf6N2E2_1240 at 1453.465 kb on - strand, within Pf6N2E2_1240 at 1453.465 kb on - strand, within Pf6N2E2_1240 at 1453.482 kb on + strand, within Pf6N2E2_1240 at 1453.560 kb on + strand at 1453.560 kb on + strand, within Pf6N2E2_1240 at 1453.560 kb on + strand, within Pf6N2E2_1240 at 1453.561 kb on - strand, within Pf6N2E2_1240 at 1453.561 kb on - strand, within Pf6N2E2_1240 at 1453.563 kb on - strand, within Pf6N2E2_1240 at 1453.563 kb on - strand, within Pf6N2E2_1240 at 1453.636 kb on + strand, within Pf6N2E2_1240 at 1453.637 kb on - strand, within Pf6N2E2_1240 at 1453.637 kb on - strand, within Pf6N2E2_1240 at 1453.694 kb on + strand, within Pf6N2E2_1240 at 1453.694 kb on + strand, within Pf6N2E2_1240 at 1453.695 kb on - strand, within Pf6N2E2_1240
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 1,451,601 + -0.5 1,451,608 - +0.3 1,451,620 + -0.1 1,451,620 + +0.8 1,451,717 + -0.6 1,451,717 + -0.1 1,451,717 + +0.5 1,451,717 + +0.1 1,451,717 + +0.3 1,451,718 - +0.4 1,451,718 - +0.3 1,451,740 + +0.2 1,451,899 + Pf6N2E2_1238 0.62 +0.6 1,451,935 + Pf6N2E2_1238 0.81 -0.0 1,451,935 + Pf6N2E2_1238 0.81 -0.2 1,451,967 + -0.3 1,451,967 + -0.2 1,451,967 + -0.1 1,451,967 + +1.3 1,451,968 - -0.7 1,451,968 - +0.4 1,451,988 + -0.3 1,451,989 - -1.3 1,452,069 - -0.2 1,452,084 + -0.6 1,452,084 + -0.5 1,452,084 + +0.7 1,452,084 + +0.4 1,452,084 + -0.9 1,452,084 + +0.7 1,452,084 + -0.3 1,452,084 + +0.1 1,452,084 + -0.1 1,452,085 - -0.3 1,452,085 - -0.4 1,452,085 - +0.1 1,452,085 - +0.5 1,452,085 - -0.1 1,452,085 - +1.1 1,452,085 - +0.6 1,452,085 - -1.0 1,452,085 - +0.5 1,452,085 - -0.4 1,452,086 + +0.0 1,452,130 + +0.1 1,452,130 + -0.2 1,452,130 + -0.7 1,452,131 - -0.1 1,452,189 - -0.9 1,452,296 - -1.3 1,452,296 - -0.7 1,452,303 + -0.4 1,452,303 + -0.8 1,452,304 - -1.3 1,452,304 - -0.5 1,452,307 + -0.9 1,452,308 - Pf6N2E2_1239 0.10 -0.7 1,452,308 - Pf6N2E2_1239 0.10 -0.1 1,452,308 - Pf6N2E2_1239 0.10 -2.0 1,452,308 - Pf6N2E2_1239 0.10 -1.2 1,452,308 - Pf6N2E2_1239 0.10 -1.4 1,452,308 - Pf6N2E2_1239 0.10 -2.0 1,452,308 - Pf6N2E2_1239 0.10 +0.2 1,452,308 - Pf6N2E2_1239 0.10 -1.7 1,452,308 - Pf6N2E2_1239 0.10 -0.3 1,452,332 - Pf6N2E2_1239 0.14 -0.3 1,452,332 - Pf6N2E2_1239 0.14 -1.0 1,452,332 - Pf6N2E2_1239 0.14 -3.5 1,452,332 - Pf6N2E2_1239 0.14 -1.8 1,452,332 - Pf6N2E2_1239 0.14 -1.5 1,452,332 - Pf6N2E2_1239 0.14 -2.0 1,452,332 - Pf6N2E2_1239 0.14 -0.5 1,452,332 - Pf6N2E2_1239 0.14 -1.6 1,452,335 + Pf6N2E2_1239 0.14 -0.4 1,452,556 - Pf6N2E2_1239 0.47 -1.6 1,452,556 - Pf6N2E2_1239 0.47 -0.4 1,452,556 - Pf6N2E2_1239 0.47 -0.0 1,452,617 - Pf6N2E2_1239 0.56 -1.7 1,452,787 + Pf6N2E2_1239 0.81 -1.3 1,452,832 + Pf6N2E2_1239 0.88 -0.4 1,452,832 + Pf6N2E2_1239 0.88 -1.2 1,452,833 - Pf6N2E2_1239 0.88 -0.6 1,452,955 + -2.6 1,452,955 + +0.2 1,452,955 + -3.0 1,452,956 - -0.2 1,452,956 - -0.2 1,452,969 + -0.5 1,452,977 + -0.8 1,452,977 + +0.0 1,452,977 + -0.7 1,452,977 + -0.4 1,452,978 - -0.1 1,452,982 - +0.7 1,452,982 - -0.8 1,453,212 - -0.5 1,453,256 + -0.2 1,453,257 - +0.6 1,453,326 + -0.4 1,453,355 - Pf6N2E2_1240 0.13 -0.4 1,453,419 - Pf6N2E2_1240 0.20 -0.0 1,453,464 + Pf6N2E2_1240 0.25 -0.2 1,453,465 - Pf6N2E2_1240 0.25 +0.0 1,453,465 - Pf6N2E2_1240 0.25 +0.4 1,453,482 + Pf6N2E2_1240 0.27 +0.8 1,453,560 + +0.9 1,453,560 + Pf6N2E2_1240 0.36 +0.6 1,453,560 + Pf6N2E2_1240 0.36 -0.2 1,453,561 - Pf6N2E2_1240 0.37 -1.2 1,453,561 - Pf6N2E2_1240 0.37 -0.9 1,453,563 - Pf6N2E2_1240 0.37 +0.0 1,453,563 - Pf6N2E2_1240 0.37 +0.1 1,453,636 + Pf6N2E2_1240 0.45 +0.2 1,453,637 - Pf6N2E2_1240 0.45 +0.6 1,453,637 - Pf6N2E2_1240 0.45 +0.1 1,453,694 + Pf6N2E2_1240 0.52 -0.2 1,453,694 + Pf6N2E2_1240 0.52 -0.8 1,453,695 - Pf6N2E2_1240 0.52 +0.8
Or see this region's nucleotide sequence