Experiment: Tetracycline 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4894 and Pf6N2E2_4895 are separated by 81 nucleotides Pf6N2E2_4895 and Pf6N2E2_4896 are separated by 98 nucleotides
Pf6N2E2_4894 - large transcriptional regulator, at 5,645,441 to 5,648,740
_4894
Pf6N2E2_4895 - Isochorismatase (EC 3.3.2.1), at 5,648,822 to 5,649,376
_4895
Pf6N2E2_4896 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 5,649,475 to 5,650,470
_4896
Position (kb) 5648
5649
5650 Strain fitness (log2 ratio) -1
0
1 at 5647.907 kb on + strand, within Pf6N2E2_4894 at 5647.907 kb on + strand, within Pf6N2E2_4894 at 5647.907 kb on + strand, within Pf6N2E2_4894 at 5647.908 kb on - strand, within Pf6N2E2_4894 at 5647.911 kb on - strand, within Pf6N2E2_4894 at 5647.938 kb on - strand, within Pf6N2E2_4894 at 5647.938 kb on - strand, within Pf6N2E2_4894 at 5647.959 kb on + strand, within Pf6N2E2_4894 at 5647.959 kb on + strand, within Pf6N2E2_4894 at 5648.033 kb on + strand, within Pf6N2E2_4894 at 5648.033 kb on + strand, within Pf6N2E2_4894 at 5648.033 kb on + strand, within Pf6N2E2_4894 at 5648.033 kb on + strand, within Pf6N2E2_4894 at 5648.034 kb on - strand, within Pf6N2E2_4894 at 5648.351 kb on + strand, within Pf6N2E2_4894 at 5648.351 kb on + strand, within Pf6N2E2_4894 at 5648.351 kb on + strand, within Pf6N2E2_4894 at 5648.351 kb on + strand, within Pf6N2E2_4894 at 5648.352 kb on - strand, within Pf6N2E2_4894 at 5648.352 kb on - strand, within Pf6N2E2_4894 at 5648.352 kb on - strand, within Pf6N2E2_4894 at 5648.352 kb on - strand, within Pf6N2E2_4894 at 5648.519 kb on - strand at 5648.530 kb on + strand at 5648.530 kb on + strand at 5648.752 kb on + strand at 5648.753 kb on - strand at 5648.753 kb on - strand at 5648.823 kb on + strand at 5648.824 kb on - strand at 5648.983 kb on - strand, within Pf6N2E2_4895 at 5649.086 kb on + strand, within Pf6N2E2_4895 at 5649.087 kb on - strand, within Pf6N2E2_4895 at 5649.087 kb on - strand, within Pf6N2E2_4895 at 5649.087 kb on - strand, within Pf6N2E2_4895 at 5649.087 kb on - strand, within Pf6N2E2_4895 at 5649.087 kb on - strand, within Pf6N2E2_4895 at 5649.087 kb on - strand, within Pf6N2E2_4895 at 5649.158 kb on - strand, within Pf6N2E2_4895 at 5649.333 kb on + strand at 5649.333 kb on + strand at 5649.334 kb on - strand at 5649.334 kb on - strand at 5649.334 kb on - strand at 5649.334 kb on - strand at 5649.334 kb on - strand at 5649.354 kb on - strand at 5649.422 kb on + strand at 5649.455 kb on + strand at 5649.455 kb on + strand at 5649.455 kb on + strand at 5649.455 kb on + strand at 5649.456 kb on - strand at 5649.456 kb on - strand at 5649.456 kb on - strand at 5649.456 kb on - strand at 5649.517 kb on + strand at 5649.517 kb on + strand at 5649.517 kb on + strand at 5649.518 kb on - strand at 5650.001 kb on + strand, within Pf6N2E2_4896 at 5650.099 kb on - strand, within Pf6N2E2_4896 at 5650.224 kb on + strand, within Pf6N2E2_4896 at 5650.224 kb on + strand, within Pf6N2E2_4896 at 5650.225 kb on - strand, within Pf6N2E2_4896 at 5650.225 kb on - strand, within Pf6N2E2_4896 at 5650.225 kb on - strand, within Pf6N2E2_4896 at 5650.225 kb on - strand, within Pf6N2E2_4896 at 5650.273 kb on + strand, within Pf6N2E2_4896 at 5650.274 kb on - strand, within Pf6N2E2_4896 at 5650.274 kb on - strand, within Pf6N2E2_4896 at 5650.274 kb on - strand, within Pf6N2E2_4896
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0001 mg/ml remove 5,647,907 + Pf6N2E2_4894 0.75 +0.3 5,647,907 + Pf6N2E2_4894 0.75 -0.6 5,647,907 + Pf6N2E2_4894 0.75 -0.1 5,647,908 - Pf6N2E2_4894 0.75 +0.4 5,647,911 - Pf6N2E2_4894 0.75 -0.3 5,647,938 - Pf6N2E2_4894 0.76 +0.5 5,647,938 - Pf6N2E2_4894 0.76 -0.1 5,647,959 + Pf6N2E2_4894 0.76 +0.4 5,647,959 + Pf6N2E2_4894 0.76 +1.0 5,648,033 + Pf6N2E2_4894 0.79 +0.5 5,648,033 + Pf6N2E2_4894 0.79 -0.5 5,648,033 + Pf6N2E2_4894 0.79 +0.4 5,648,033 + Pf6N2E2_4894 0.79 +0.3 5,648,034 - Pf6N2E2_4894 0.79 +0.3 5,648,351 + Pf6N2E2_4894 0.88 -0.5 5,648,351 + Pf6N2E2_4894 0.88 -0.8 5,648,351 + Pf6N2E2_4894 0.88 -0.2 5,648,351 + Pf6N2E2_4894 0.88 +0.1 5,648,352 - Pf6N2E2_4894 0.88 +0.1 5,648,352 - Pf6N2E2_4894 0.88 -0.0 5,648,352 - Pf6N2E2_4894 0.88 -0.1 5,648,352 - Pf6N2E2_4894 0.88 +0.4 5,648,519 - +0.2 5,648,530 + +0.8 5,648,530 + +0.2 5,648,752 + -0.2 5,648,753 - +0.1 5,648,753 - +0.5 5,648,823 + +0.8 5,648,824 - +0.2 5,648,983 - Pf6N2E2_4895 0.29 +0.2 5,649,086 + Pf6N2E2_4895 0.48 +0.7 5,649,087 - Pf6N2E2_4895 0.48 +0.9 5,649,087 - Pf6N2E2_4895 0.48 +0.1 5,649,087 - Pf6N2E2_4895 0.48 +0.7 5,649,087 - Pf6N2E2_4895 0.48 -0.4 5,649,087 - Pf6N2E2_4895 0.48 +0.7 5,649,087 - Pf6N2E2_4895 0.48 +0.0 5,649,158 - Pf6N2E2_4895 0.61 +1.2 5,649,333 + -0.9 5,649,333 + -0.4 5,649,334 - +0.4 5,649,334 - +0.0 5,649,334 - +0.0 5,649,334 - +0.5 5,649,334 - -0.4 5,649,354 - +0.3 5,649,422 + -0.9 5,649,455 + -0.5 5,649,455 + +0.3 5,649,455 + +0.6 5,649,455 + -0.3 5,649,456 - +0.2 5,649,456 - +0.2 5,649,456 - +0.5 5,649,456 - +0.1 5,649,517 + +0.4 5,649,517 + +0.3 5,649,517 + -0.0 5,649,518 - +0.5 5,650,001 + Pf6N2E2_4896 0.53 -0.1 5,650,099 - Pf6N2E2_4896 0.63 +0.4 5,650,224 + Pf6N2E2_4896 0.75 -1.7 5,650,224 + Pf6N2E2_4896 0.75 +0.4 5,650,225 - Pf6N2E2_4896 0.75 +0.4 5,650,225 - Pf6N2E2_4896 0.75 +0.5 5,650,225 - Pf6N2E2_4896 0.75 -0.8 5,650,225 - Pf6N2E2_4896 0.75 +0.5 5,650,273 + Pf6N2E2_4896 0.80 -0.1 5,650,274 - Pf6N2E2_4896 0.80 +0.8 5,650,274 - Pf6N2E2_4896 0.80 +0.2 5,650,274 - Pf6N2E2_4896 0.80 -0.9
Or see this region's nucleotide sequence