Experiment: Chloramphenicol 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_102 and Pf6N2E2_103 are separated by 355 nucleotides Pf6N2E2_103 and Pf6N2E2_104 overlap by 1 nucleotides
Pf6N2E2_102 - sigma-54 dependent transcriptional regulator, at 146,344 to 148,188
_102
Pf6N2E2_103 - Various polyols ABC transporter, ATP-binding component, at 148,544 to 149,638
_103
Pf6N2E2_104 - Glycerol-3-phosphate ABC transporter, ATP-binding protein UgpC (TC 3.A.1.1.3), at 149,638 to 150,747
_104
Position (kb) 148
149
150 Strain fitness (log2 ratio) -1
0
1 at 147.686 kb on + strand, within Pf6N2E2_102 at 147.686 kb on + strand, within Pf6N2E2_102 at 147.687 kb on - strand, within Pf6N2E2_102 at 147.687 kb on - strand, within Pf6N2E2_102 at 147.687 kb on - strand, within Pf6N2E2_102 at 148.214 kb on + strand at 148.214 kb on + strand at 148.265 kb on + strand at 148.275 kb on + strand at 148.275 kb on + strand at 148.275 kb on + strand at 148.275 kb on + strand at 148.529 kb on + strand at 148.530 kb on - strand at 148.530 kb on - strand at 148.652 kb on - strand at 149.037 kb on - strand, within Pf6N2E2_103 at 149.037 kb on - strand, within Pf6N2E2_103 at 149.204 kb on + strand, within Pf6N2E2_103 at 149.383 kb on + strand, within Pf6N2E2_103 at 149.383 kb on + strand, within Pf6N2E2_103 at 149.384 kb on - strand, within Pf6N2E2_103 at 149.384 kb on - strand, within Pf6N2E2_103 at 149.525 kb on + strand, within Pf6N2E2_103 at 149.525 kb on + strand, within Pf6N2E2_103 at 149.552 kb on + strand at 149.552 kb on + strand at 149.553 kb on - strand at 149.675 kb on - strand at 149.675 kb on - strand at 149.914 kb on + strand, within Pf6N2E2_104 at 149.914 kb on + strand, within Pf6N2E2_104 at 149.914 kb on + strand, within Pf6N2E2_104 at 149.915 kb on - strand, within Pf6N2E2_104 at 149.915 kb on - strand, within Pf6N2E2_104 at 149.915 kb on - strand, within Pf6N2E2_104 at 149.942 kb on + strand, within Pf6N2E2_104 at 149.943 kb on - strand, within Pf6N2E2_104 at 149.943 kb on - strand, within Pf6N2E2_104 at 150.223 kb on + strand, within Pf6N2E2_104 at 150.224 kb on - strand, within Pf6N2E2_104 at 150.280 kb on + strand, within Pf6N2E2_104 at 150.281 kb on - strand, within Pf6N2E2_104 at 150.281 kb on - strand, within Pf6N2E2_104 at 150.532 kb on + strand, within Pf6N2E2_104 at 150.533 kb on - strand, within Pf6N2E2_104 at 150.583 kb on + strand, within Pf6N2E2_104 at 150.583 kb on + strand, within Pf6N2E2_104 at 150.584 kb on - strand, within Pf6N2E2_104 at 150.584 kb on - strand, within Pf6N2E2_104 at 150.627 kb on - strand, within Pf6N2E2_104
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.004 mg/ml remove 147,686 + Pf6N2E2_102 0.73 -1.1 147,686 + Pf6N2E2_102 0.73 +0.5 147,687 - Pf6N2E2_102 0.73 +0.2 147,687 - Pf6N2E2_102 0.73 +0.9 147,687 - Pf6N2E2_102 0.73 +0.3 148,214 + +0.3 148,214 + +0.4 148,265 + -1.0 148,275 + +0.8 148,275 + +0.8 148,275 + -0.6 148,275 + +0.1 148,529 + +0.1 148,530 - -0.5 148,530 - -0.8 148,652 - -0.5 149,037 - Pf6N2E2_103 0.45 -0.0 149,037 - Pf6N2E2_103 0.45 +0.7 149,204 + Pf6N2E2_103 0.60 -1.0 149,383 + Pf6N2E2_103 0.77 +0.3 149,383 + Pf6N2E2_103 0.77 +0.6 149,384 - Pf6N2E2_103 0.77 -1.0 149,384 - Pf6N2E2_103 0.77 -0.6 149,525 + Pf6N2E2_103 0.90 +0.3 149,525 + Pf6N2E2_103 0.90 -0.4 149,552 + -0.3 149,552 + +0.1 149,553 - +0.5 149,675 - -0.2 149,675 - +0.4 149,914 + Pf6N2E2_104 0.25 -0.2 149,914 + Pf6N2E2_104 0.25 +0.3 149,914 + Pf6N2E2_104 0.25 +0.2 149,915 - Pf6N2E2_104 0.25 -0.7 149,915 - Pf6N2E2_104 0.25 +0.0 149,915 - Pf6N2E2_104 0.25 +0.7 149,942 + Pf6N2E2_104 0.27 +0.7 149,943 - Pf6N2E2_104 0.27 +0.3 149,943 - Pf6N2E2_104 0.27 +0.3 150,223 + Pf6N2E2_104 0.53 +0.3 150,224 - Pf6N2E2_104 0.53 -0.2 150,280 + Pf6N2E2_104 0.58 -0.4 150,281 - Pf6N2E2_104 0.58 +1.6 150,281 - Pf6N2E2_104 0.58 -0.4 150,532 + Pf6N2E2_104 0.81 -0.2 150,533 - Pf6N2E2_104 0.81 +0.3 150,583 + Pf6N2E2_104 0.85 -0.8 150,583 + Pf6N2E2_104 0.85 +0.3 150,584 - Pf6N2E2_104 0.85 -0.4 150,584 - Pf6N2E2_104 0.85 +0.5 150,627 - Pf6N2E2_104 0.89 -0.6
Or see this region's nucleotide sequence