Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF740 and GFF741 are separated by 264 nucleotides GFF741 and GFF742 are separated by 346 nucleotides
GFF740 - Putative LysR family transcriptional regulator, at 99,087 to 99,977
GFF740
GFF741 - D-beta-hydroxybutyrate permease, at 100,242 to 100,667
GFF741
GFF742 - Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2), at 101,014 to 101,694
GFF742
Position (kb) 100
101 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 99.407 kb on - strand, within GFF740 at 99.677 kb on + strand, within GFF740 at 99.779 kb on + strand, within GFF740 at 99.821 kb on + strand, within GFF740 at 99.965 kb on + strand at 100.177 kb on + strand at 100.177 kb on + strand at 100.178 kb on - strand at 100.178 kb on - strand at 100.178 kb on - strand at 100.295 kb on + strand, within GFF741 at 100.296 kb on - strand, within GFF741 at 100.377 kb on + strand, within GFF741 at 100.480 kb on - strand, within GFF741 at 100.538 kb on + strand, within GFF741 at 100.542 kb on + strand, within GFF741 at 100.678 kb on - strand at 100.801 kb on - strand at 101.010 kb on + strand at 101.140 kb on + strand, within GFF742 at 101.197 kb on + strand, within GFF742 at 101.197 kb on + strand, within GFF742 at 101.197 kb on + strand, within GFF742 at 101.198 kb on - strand, within GFF742 at 101.353 kb on + strand, within GFF742 at 101.354 kb on - strand, within GFF742 at 101.416 kb on + strand, within GFF742 at 101.419 kb on + strand, within GFF742 at 101.596 kb on - strand, within GFF742 at 101.596 kb on - strand, within GFF742 at 101.599 kb on + strand, within GFF742 at 101.599 kb on + strand, within GFF742 at 101.626 kb on + strand, within GFF742 at 101.630 kb on + strand at 101.630 kb on + strand at 101.630 kb on + strand at 101.630 kb on + strand at 101.631 kb on - strand at 101.631 kb on - strand at 101.631 kb on - strand at 101.631 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 99,407 - GFF740 0.36 -1.0 99,677 + GFF740 0.66 -0.9 99,779 + GFF740 0.78 -0.1 99,821 + GFF740 0.82 +1.5 99,965 + -0.4 100,177 + +3.2 100,177 + +0.1 100,178 - +0.4 100,178 - +0.3 100,178 - -2.3 100,295 + GFF741 0.12 -1.3 100,296 - GFF741 0.13 -0.7 100,377 + GFF741 0.32 -0.4 100,480 - GFF741 0.56 -1.8 100,538 + GFF741 0.69 -0.6 100,542 + GFF741 0.70 +0.1 100,678 - +0.7 100,801 - -0.1 101,010 + +0.1 101,140 + GFF742 0.19 +0.5 101,197 + GFF742 0.27 +0.1 101,197 + GFF742 0.27 +0.9 101,197 + GFF742 0.27 +1.9 101,198 - GFF742 0.27 -0.1 101,353 + GFF742 0.50 +0.1 101,354 - GFF742 0.50 -0.0 101,416 + GFF742 0.59 +0.9 101,419 + GFF742 0.59 -0.8 101,596 - GFF742 0.85 +0.8 101,596 - GFF742 0.85 -0.3 101,599 + GFF742 0.86 +0.0 101,599 + GFF742 0.86 +0.3 101,626 + GFF742 0.90 +0.3 101,630 + +0.4 101,630 + +0.5 101,630 + +0.1 101,630 + +0.5 101,631 - +0.3 101,631 - +0.1 101,631 - -0.8 101,631 - +0.7
Or see this region's nucleotide sequence