Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3482 and GFF3483 are separated by 95 nucleotides GFF3483 and GFF3484 are separated by 173 nucleotides GFF3484 and GFF3485 are separated by 117 nucleotides
GFF3482 - L-asparaginase I, cytoplasmic (EC 3.5.1.1), at 233,851 to 234,867
GFF3482
GFF3483 - Signal peptide peptidase SppA (EC 3.4.21.-), at 234,963 to 236,819
GFF3483
GFF3484 - FIG002003: Protein YdjA, at 236,993 to 237,544
GFF3484
GFF3485 - Selenide,water dikinase (EC 2.7.9.3), at 237,662 to 238,705
GFF3485
Position (kb) 234
235
236
237 Strain fitness (log2 ratio) -1
0
1
2 at 234.143 kb on + strand, within GFF3482 at 234.190 kb on + strand, within GFF3482 at 234.250 kb on - strand, within GFF3482 at 234.263 kb on - strand, within GFF3482 at 234.412 kb on - strand, within GFF3482 at 234.414 kb on + strand, within GFF3482 at 234.427 kb on + strand, within GFF3482 at 234.477 kb on + strand, within GFF3482 at 234.623 kb on + strand, within GFF3482 at 234.658 kb on - strand, within GFF3482 at 234.681 kb on + strand, within GFF3482 at 234.682 kb on - strand, within GFF3482 at 234.795 kb on + strand at 234.805 kb on - strand at 234.826 kb on - strand at 234.849 kb on - strand at 234.870 kb on + strand at 234.898 kb on - strand at 234.898 kb on - strand at 234.901 kb on - strand at 235.031 kb on + strand at 235.145 kb on + strand at 235.146 kb on - strand at 235.146 kb on - strand at 235.174 kb on + strand, within GFF3483 at 235.561 kb on + strand, within GFF3483 at 235.561 kb on + strand, within GFF3483 at 235.561 kb on + strand, within GFF3483 at 235.561 kb on + strand, within GFF3483 at 235.730 kb on + strand, within GFF3483 at 235.766 kb on + strand, within GFF3483 at 235.883 kb on - strand, within GFF3483 at 235.996 kb on + strand, within GFF3483 at 236.040 kb on - strand, within GFF3483 at 236.104 kb on + strand, within GFF3483 at 236.258 kb on - strand, within GFF3483 at 236.341 kb on + strand, within GFF3483 at 236.421 kb on - strand, within GFF3483 at 236.422 kb on + strand, within GFF3483 at 236.423 kb on - strand, within GFF3483 at 236.484 kb on - strand, within GFF3483 at 236.487 kb on - strand, within GFF3483 at 236.634 kb on - strand at 236.821 kb on + strand at 236.821 kb on + strand at 236.822 kb on - strand at 236.851 kb on - strand at 236.873 kb on - strand at 236.967 kb on + strand at 237.070 kb on - strand, within GFF3484 at 237.262 kb on + strand, within GFF3484 at 237.602 kb on + strand at 237.603 kb on - strand at 237.603 kb on - strand at 237.634 kb on - strand at 237.746 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 234,143 + GFF3482 0.29 +1.5 234,190 + GFF3482 0.33 -0.3 234,250 - GFF3482 0.39 +0.7 234,263 - GFF3482 0.41 -1.7 234,412 - GFF3482 0.55 +0.7 234,414 + GFF3482 0.55 +0.2 234,427 + GFF3482 0.57 +0.1 234,477 + GFF3482 0.62 +0.7 234,623 + GFF3482 0.76 -0.7 234,658 - GFF3482 0.79 -1.0 234,681 + GFF3482 0.82 -0.5 234,682 - GFF3482 0.82 +0.1 234,795 + -0.7 234,805 - +0.6 234,826 - -0.4 234,849 - -0.2 234,870 + +0.5 234,898 - -1.4 234,898 - +0.7 234,901 - -1.3 235,031 + +0.1 235,145 + -0.3 235,146 - -0.1 235,146 - +0.5 235,174 + GFF3483 0.11 -0.2 235,561 + GFF3483 0.32 -0.5 235,561 + GFF3483 0.32 +0.3 235,561 + GFF3483 0.32 -0.3 235,561 + GFF3483 0.32 -1.1 235,730 + GFF3483 0.41 -0.2 235,766 + GFF3483 0.43 -0.9 235,883 - GFF3483 0.50 -0.2 235,996 + GFF3483 0.56 -1.0 236,040 - GFF3483 0.58 +1.0 236,104 + GFF3483 0.61 +1.0 236,258 - GFF3483 0.70 -0.0 236,341 + GFF3483 0.74 +1.1 236,421 - GFF3483 0.79 -1.3 236,422 + GFF3483 0.79 -0.9 236,423 - GFF3483 0.79 -0.3 236,484 - GFF3483 0.82 -1.1 236,487 - GFF3483 0.82 +0.3 236,634 - -0.2 236,821 + -0.2 236,821 + +0.2 236,822 - +1.0 236,851 - -0.2 236,873 - +1.1 236,967 + +0.7 237,070 - GFF3484 0.14 +0.3 237,262 + GFF3484 0.49 -1.3 237,602 + +2.0 237,603 - +1.3 237,603 - -0.7 237,634 - -0.2 237,746 - +1.0
Or see this region's nucleotide sequence