Strain Fitness in Pseudomonas fluorescens GW456-L13 around PfGW456L13_595

Experiment: Vancomycin 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPfGW456L13_594 and PfGW456L13_595 are separated by 74 nucleotidesPfGW456L13_595 and PfGW456L13_596 are separated by 60 nucleotides PfGW456L13_594 - DNA polymerase I (EC 2.7.7.7), at 687,197 to 690,001 _594 PfGW456L13_595 - FIG00953549: hypothetical protein, at 690,076 to 690,363 _595 PfGW456L13_596 - Homoserine kinase (EC 2.7.1.39), at 690,424 to 691,374 _596 Position (kb) 690 691Strain fitness (log2 ratio) -3 -2 -1 0 1at 690.072 kb on + strandat 690.073 kb on - strandat 690.073 kb on - strandat 690.086 kb on + strandat 690.086 kb on + strandat 690.087 kb on - strandat 690.087 kb on - strandat 690.087 kb on - strandat 690.195 kb on + strand, within PfGW456L13_595at 690.196 kb on - strand, within PfGW456L13_595at 690.232 kb on + strand, within PfGW456L13_595at 690.269 kb on - strand, within PfGW456L13_595at 690.327 kb on + strand, within PfGW456L13_595at 690.361 kb on + strandat 690.361 kb on + strandat 690.361 kb on + strandat 690.362 kb on - strandat 690.419 kb on + strandat 690.419 kb on + strandat 690.419 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Vancomycin 0.5 mg/ml
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690,072 + -0.4
690,073 - -0.6
690,073 - -1.4
690,086 + -0.0
690,086 + -1.8
690,087 - -3.0
690,087 - -0.9
690,087 - -1.5
690,195 + PfGW456L13_595 0.41 -1.4
690,196 - PfGW456L13_595 0.42 -2.7
690,232 + PfGW456L13_595 0.54 -1.2
690,269 - PfGW456L13_595 0.67 -1.2
690,327 + PfGW456L13_595 0.87 -0.3
690,361 + -0.0
690,361 + -1.8
690,361 + -0.5
690,362 - -0.6
690,419 + -3.0
690,419 + -2.2
690,419 + -1.9

Or see this region's nucleotide sequence