Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4427 and GFF4428 are separated by 78 nucleotides GFF4428 and GFF4429 are separated by 58 nucleotides
GFF4427 - Allantoin permease, at 212,639 to 214,093
GFF4427
GFF4428 - Allantoinase (EC 3.5.2.5), at 214,172 to 215,533
GFF4428
GFF4429 - Xanthine permease, at 215,592 to 216,890
GFF4429
Position (kb) 214
215
216 Strain fitness (log2 ratio) -2
-1
0
1 at 213.455 kb on - strand, within GFF4427 at 213.455 kb on - strand, within GFF4427 at 213.456 kb on + strand, within GFF4427 at 213.457 kb on - strand, within GFF4427 at 213.500 kb on - strand, within GFF4427 at 214.011 kb on + strand at 214.185 kb on + strand at 214.349 kb on - strand, within GFF4428 at 214.556 kb on + strand, within GFF4428 at 214.557 kb on - strand, within GFF4428 at 214.557 kb on - strand, within GFF4428 at 214.725 kb on + strand, within GFF4428 at 214.726 kb on - strand, within GFF4428 at 214.802 kb on + strand, within GFF4428 at 214.802 kb on + strand, within GFF4428 at 214.802 kb on + strand, within GFF4428 at 214.803 kb on - strand, within GFF4428 at 215.234 kb on - strand, within GFF4428 at 215.308 kb on + strand, within GFF4428 at 215.309 kb on - strand, within GFF4428 at 215.342 kb on - strand, within GFF4428 at 215.361 kb on - strand, within GFF4428 at 215.361 kb on - strand, within GFF4428 at 215.361 kb on - strand, within GFF4428 at 215.388 kb on - strand, within GFF4428 at 215.422 kb on + strand at 215.438 kb on - strand at 215.468 kb on + strand at 215.469 kb on - strand at 215.469 kb on - strand at 215.531 kb on + strand at 215.580 kb on + strand at 215.604 kb on - strand at 215.832 kb on - strand, within GFF4429 at 215.954 kb on - strand, within GFF4429 at 216.077 kb on + strand, within GFF4429 at 216.168 kb on - strand, within GFF4429 at 216.245 kb on - strand, within GFF4429 at 216.309 kb on - strand, within GFF4429 at 216.380 kb on + strand, within GFF4429 at 216.496 kb on + strand, within GFF4429 at 216.497 kb on - strand, within GFF4429
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 213,455 - GFF4427 0.56 -0.8 213,455 - GFF4427 0.56 -1.0 213,456 + GFF4427 0.56 +0.1 213,457 - GFF4427 0.56 +0.5 213,500 - GFF4427 0.59 -1.8 214,011 + -0.5 214,185 + -0.1 214,349 - GFF4428 0.13 -0.7 214,556 + GFF4428 0.28 -0.8 214,557 - GFF4428 0.28 -1.2 214,557 - GFF4428 0.28 +0.5 214,725 + GFF4428 0.41 -1.3 214,726 - GFF4428 0.41 -0.4 214,802 + GFF4428 0.46 -0.7 214,802 + GFF4428 0.46 -1.1 214,802 + GFF4428 0.46 -0.8 214,803 - GFF4428 0.46 -2.6 215,234 - GFF4428 0.78 -2.1 215,308 + GFF4428 0.83 -0.0 215,309 - GFF4428 0.83 -0.5 215,342 - GFF4428 0.86 +0.7 215,361 - GFF4428 0.87 -1.6 215,361 - GFF4428 0.87 -1.0 215,361 - GFF4428 0.87 -1.5 215,388 - GFF4428 0.89 +0.3 215,422 + +0.9 215,438 - +0.5 215,468 + +0.8 215,469 - +0.8 215,469 - -0.6 215,531 + +0.3 215,580 + +0.0 215,604 - +0.7 215,832 - GFF4429 0.18 -1.9 215,954 - GFF4429 0.28 +0.5 216,077 + GFF4429 0.37 +0.3 216,168 - GFF4429 0.44 +1.1 216,245 - GFF4429 0.50 -1.1 216,309 - GFF4429 0.55 +0.4 216,380 + GFF4429 0.61 -1.1 216,496 + GFF4429 0.70 +0.3 216,497 - GFF4429 0.70 -1.3
Or see this region's nucleotide sequence