Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3363 and GFF3364 are separated by 14 nucleotides GFF3364 and GFF3365 are separated by 256 nucleotides
GFF3363 - Flagellar hook-associated protein FlgK, at 127,520 to 129,181
GFF3363
GFF3364 - Flagellar hook-associated protein FlgL, at 129,196 to 130,149
GFF3364
GFF3365 - Ribonuclease E (EC 3.1.26.12), at 130,406 to 133,609
GFF3365
Position (kb) 129
130
131 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 128.218 kb on + strand, within GFF3363 at 128.218 kb on + strand, within GFF3363 at 128.298 kb on - strand, within GFF3363 at 128.298 kb on - strand, within GFF3363 at 128.404 kb on + strand, within GFF3363 at 128.405 kb on - strand, within GFF3363 at 128.408 kb on - strand, within GFF3363 at 128.527 kb on + strand, within GFF3363 at 128.527 kb on + strand, within GFF3363 at 128.527 kb on + strand, within GFF3363 at 128.528 kb on - strand, within GFF3363 at 128.536 kb on + strand, within GFF3363 at 128.539 kb on + strand, within GFF3363 at 128.539 kb on + strand, within GFF3363 at 128.606 kb on + strand, within GFF3363 at 128.608 kb on + strand, within GFF3363 at 128.608 kb on + strand, within GFF3363 at 128.919 kb on + strand, within GFF3363 at 128.919 kb on + strand, within GFF3363 at 128.920 kb on - strand, within GFF3363 at 129.028 kb on + strand at 129.028 kb on + strand at 129.040 kb on + strand at 129.113 kb on + strand at 129.133 kb on - strand at 129.220 kb on + strand at 129.347 kb on + strand, within GFF3364 at 129.379 kb on + strand, within GFF3364 at 129.595 kb on + strand, within GFF3364 at 129.676 kb on - strand, within GFF3364 at 130.063 kb on + strand at 130.064 kb on - strand at 130.085 kb on + strand at 130.243 kb on + strand at 130.253 kb on - strand at 130.281 kb on + strand at 130.402 kb on + strand at 130.402 kb on + strand at 130.540 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 128,218 + GFF3363 0.42 -0.5 128,218 + GFF3363 0.42 +0.9 128,298 - GFF3363 0.47 -0.6 128,298 - GFF3363 0.47 -0.3 128,404 + GFF3363 0.53 +1.2 128,405 - GFF3363 0.53 +0.7 128,408 - GFF3363 0.53 +0.0 128,527 + GFF3363 0.61 +0.2 128,527 + GFF3363 0.61 +0.1 128,527 + GFF3363 0.61 -0.5 128,528 - GFF3363 0.61 +1.4 128,536 + GFF3363 0.61 -0.1 128,539 + GFF3363 0.61 -0.5 128,539 + GFF3363 0.61 -2.5 128,606 + GFF3363 0.65 -0.6 128,608 + GFF3363 0.65 +0.6 128,608 + GFF3363 0.65 -0.7 128,919 + GFF3363 0.84 -1.5 128,919 + GFF3363 0.84 -0.0 128,920 - GFF3363 0.84 +4.2 129,028 + -0.0 129,028 + -1.3 129,040 + +0.2 129,113 + +0.0 129,133 - -1.6 129,220 + +0.2 129,347 + GFF3364 0.16 +0.3 129,379 + GFF3364 0.19 -2.1 129,595 + GFF3364 0.42 -1.1 129,676 - GFF3364 0.50 -1.3 130,063 + +0.0 130,064 - +1.6 130,085 + +0.2 130,243 + -1.9 130,253 - -0.0 130,281 + +0.1 130,402 + +0.4 130,402 + -1.6 130,540 - +0.5
Or see this region's nucleotide sequence