Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3146 and GFF3147 are separated by 24 nucleotides GFF3147 and GFF3148 overlap by 4 nucleotides GFF3148 and GFF3149 are separated by 206 nucleotides
GFF3146 - '3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17)' transl_table=11, at 151,598 to 152,425
GFF3146
GFF3147 - FIG074102: hypothetical protein, at 152,450 to 152,872
GFF3147
GFF3148 - ADP-ribose pyrophosphatase (EC 3.6.1.13), at 152,869 to 153,501
GFF3148
GFF3149 - Type I secretion outer membrane protein, TolC precursor, at 153,708 to 155,177
GFF3149
Position (kb)
152
153
154 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 152.436 kb on + strand at 152.568 kb on + strand, within GFF3147 at 152.664 kb on + strand, within GFF3147 at 152.664 kb on + strand, within GFF3147 at 152.704 kb on + strand, within GFF3147 at 152.744 kb on + strand, within GFF3147 at 153.007 kb on - strand, within GFF3148 at 153.037 kb on - strand, within GFF3148 at 153.076 kb on - strand, within GFF3148 at 153.076 kb on - strand, within GFF3148 at 153.113 kb on + strand, within GFF3148 at 153.205 kb on + strand, within GFF3148 at 153.522 kb on - strand at 153.529 kb on - strand at 153.529 kb on - strand at 153.603 kb on - strand at 153.684 kb on - strand at 153.763 kb on + strand at 153.764 kb on - strand at 153.921 kb on + strand, within GFF3149 at 153.950 kb on + strand, within GFF3149 at 153.998 kb on - strand, within GFF3149 at 154.005 kb on - strand, within GFF3149 at 154.065 kb on + strand, within GFF3149 at 154.116 kb on - strand, within GFF3149 at 154.146 kb on + strand, within GFF3149 at 154.169 kb on + strand, within GFF3149 at 154.174 kb on - strand, within GFF3149 at 154.255 kb on - strand, within GFF3149 at 154.430 kb on + strand, within GFF3149
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 152,436 + -0.4 152,568 + GFF3147 0.28 +0.6 152,664 + GFF3147 0.51 -1.1 152,664 + GFF3147 0.51 -0.3 152,704 + GFF3147 0.60 -0.5 152,744 + GFF3147 0.70 -1.2 153,007 - GFF3148 0.22 -0.5 153,037 - GFF3148 0.27 -2.0 153,076 - GFF3148 0.33 -1.0 153,076 - GFF3148 0.33 +0.3 153,113 + GFF3148 0.39 -1.5 153,205 + GFF3148 0.53 -1.0 153,522 - +0.8 153,529 - +0.2 153,529 - -1.6 153,603 - -0.5 153,684 - +0.6 153,763 + -0.8 153,764 - +0.8 153,921 + GFF3149 0.14 -0.9 153,950 + GFF3149 0.16 -0.7 153,998 - GFF3149 0.20 -0.7 154,005 - GFF3149 0.20 -2.9 154,065 + GFF3149 0.24 -1.9 154,116 - GFF3149 0.28 +0.1 154,146 + GFF3149 0.30 +0.8 154,169 + GFF3149 0.31 -3.1 154,174 - GFF3149 0.32 -3.0 154,255 - GFF3149 0.37 -0.3 154,430 + GFF3149 0.49 -1.3
Or see this region's nucleotide sequence