Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2965 and GFF2966 are separated by 134 nucleotides GFF2966 and GFF2967 are separated by 117 nucleotides GFF2967 and GFF2968 are separated by 5 nucleotides
GFF2965 - N-acetylneuraminate lyase (EC 4.1.3.3), at 49,595 to 50,488
GFF2965
GFF2966 - Transcriptional regulator NanR, at 50,623 to 51,405
GFF2966
GFF2967 - ClpXP protease specificity-enhancing factor / Stringent starvation protein B, at 51,523 to 52,023
GFF2967
GFF2968 - Stringent starvation protein A, at 52,029 to 52,667
GFF2968
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 49.676 kb on - strand at 49.676 kb on - strand at 49.706 kb on - strand, within GFF2965 at 49.818 kb on - strand, within GFF2965 at 49.860 kb on + strand, within GFF2965 at 49.861 kb on - strand, within GFF2965 at 49.861 kb on - strand, within GFF2965 at 49.930 kb on - strand, within GFF2965 at 49.930 kb on - strand, within GFF2965 at 50.030 kb on - strand, within GFF2965 at 50.054 kb on + strand, within GFF2965 at 50.079 kb on + strand, within GFF2965 at 50.079 kb on + strand, within GFF2965 at 50.080 kb on - strand, within GFF2965 at 50.160 kb on + strand, within GFF2965 at 50.161 kb on - strand, within GFF2965 at 50.197 kb on - strand, within GFF2965 at 50.361 kb on + strand, within GFF2965 at 50.362 kb on - strand, within GFF2965 at 50.488 kb on + strand at 50.489 kb on - strand at 50.489 kb on - strand at 50.492 kb on + strand at 50.514 kb on - strand at 50.517 kb on - strand at 50.770 kb on - strand, within GFF2966 at 50.941 kb on - strand, within GFF2966 at 51.233 kb on - strand, within GFF2966 at 51.233 kb on - strand, within GFF2966 at 51.354 kb on + strand at 51.440 kb on - strand at 51.448 kb on + strand at 51.449 kb on - strand at 51.673 kb on + strand, within GFF2967 at 51.891 kb on - strand, within GFF2967 at 51.891 kb on - strand, within GFF2967 at 52.110 kb on - strand, within GFF2968 at 52.112 kb on - strand, within GFF2968 at 52.189 kb on + strand, within GFF2968 at 52.335 kb on - strand, within GFF2968
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 49,676 - -0.3 49,676 - -0.3 49,706 - GFF2965 0.12 -2.0 49,818 - GFF2965 0.25 +0.3 49,860 + GFF2965 0.30 +1.4 49,861 - GFF2965 0.30 -1.0 49,861 - GFF2965 0.30 -1.2 49,930 - GFF2965 0.37 -1.7 49,930 - GFF2965 0.37 -0.0 50,030 - GFF2965 0.49 -0.8 50,054 + GFF2965 0.51 +1.2 50,079 + GFF2965 0.54 -0.1 50,079 + GFF2965 0.54 +0.3 50,080 - GFF2965 0.54 -2.7 50,160 + GFF2965 0.63 -0.1 50,161 - GFF2965 0.63 -0.3 50,197 - GFF2965 0.67 +0.0 50,361 + GFF2965 0.86 -0.8 50,362 - GFF2965 0.86 +0.2 50,488 + -0.2 50,489 - -0.8 50,489 - -2.3 50,492 + +0.3 50,514 - +0.2 50,517 - +0.5 50,770 - GFF2966 0.19 -1.5 50,941 - GFF2966 0.41 -0.7 51,233 - GFF2966 0.78 -0.5 51,233 - GFF2966 0.78 -0.6 51,354 + +0.3 51,440 - +2.0 51,448 + -1.9 51,449 - +1.0 51,673 + GFF2967 0.30 +0.1 51,891 - GFF2967 0.73 -0.9 51,891 - GFF2967 0.73 +0.1 52,110 - GFF2968 0.13 +2.0 52,112 - GFF2968 0.13 +2.1 52,189 + GFF2968 0.25 +2.3 52,335 - GFF2968 0.48 +2.1
Or see this region's nucleotide sequence